Pinus armandi franch is a unique specialty plant in China and its seed oil contains high levels of essential fatty acids (EFA), particularly linoleic acid (LA), which has several pharmaceutical properties. Pinus armandi franch seed oil is a nice resource of linoleic acid with a content of 63% of the total fatty acids. Linoleic acid is an essential fatty acid, whose absence in a normal diet is responsible for the development of various abnormal disorders. This work reported purified LA from Pinus armandi franch seed oil could lower MDA content of lipid peroxidation on rats with hyperlipidemia significantly. TAC activity of liver, heart and serum was enhanced significantly, as well as SOD activity was increased. It demonstrated purified LA from Pinus armandi franch seed oil could improve antioxidant levels of hyperlipidemia rats effectively, enhance the activity of antioxidant enzyme and reduce the content of lipid peroxide, thereby improving lipid metabolism.

Safflower seed oil is known to be one of the richest natural sources of linoleic acid (LA), with values of up to 69.5% of the total fatty acids. Linoleic acid is known to has important biological activity and its absence in a normal diet has been described as responsible for the development of a wide variety of diseases. This work reported purified LA from safflower seed oil could lower blood liquids level of rats with hyperlipidemia. It showed that purified LA from safflower seed oil could decrease the serum total cholesterol and triglyceride concentration significantly, as well as increase the serum high density lipoprotein cholesterol. The serum atherosclerosis index (AI) was decreased remarkably. It demonstrated purified LA from safflower seed oil could improve metabolism of serum lipids and it could play active role of preventing atherosclerosis.

Flaxseed oil contains significant amounts of essential fatty acid, α-linolenic acid (ALA) with a content of 61% of the total fatty acid. ALA is attracting increasing attention because of their importance to human health. ALA has various physiological activities whose absence in the diet is responsible for the development of a wide variety of abnormalities. Highly purified ALA is required on pharmaceutical applications. This work reports as recovery of highly purified ALA from flaxseed oil by means of a process, which involves simultaneous oil saponification–extraction, followed by the ethyl esterification of fatty acids. Thereafter, the polyunsaturated fatty acids (PUFA) were concentrated by molecular distillation method, and ethyl esters below ALA were fractionated from ethyl–PUFA concentrate by mean of open column chromatography with silver–silica gel as stationary phase. The recovery in the combined process was 79%, and the final purity was 94.7%. Therefore, highly pure PUFA ALA could be procured by argentated silica gel chromatography column.