We present a method to make an accurate and dense reconstruction from the input of video captured by a free moving handheld camera in real time. By the method firstly, the positions of the camera and sparse 3D points are estimated by simultaneous localization mapping. Then the depth maps of selected reference frames are computed from corresponding camera bundles. Lastly a novel linear algorithm is also proposed to integrate all the depth maps into dense meshes partially. The main contributions of this paper are in the following points: the reference frames and corresponding camera bundles are able to be selected automatically, then accurate and smooth depth maps are generated in real time, and the depth maps are merged into a dense mesh by using a linear algorithm based on the error clouds optimization. Our algorithm is implemented on dual CPU and graphics processing unit in a parallel framework for improving the performance. Copyright © 2013 John Wiley & Sons, Ltd.

Our team's pharmacological and clinical trials proved that ligustrazine/borneol spray had a definite effect on ischemic stroke (IS). To solve the shortcomings of ligustrazine/ borneol spray, such as low bioavailability, short half-life, and poor compatibility between borneol and ligustrazine, ligustrazine-loaded borneol liposomes (LIP@TMP) were successfully prepared by a thin-film ultrasonication method. The average particle size of LIP@ TMP was 282.4 ± 3.6 nm, the drug loading rate was 14.5 ± 0.6%, and the entrapment efficiency was 42.7 ± 1.0%, which had excellent stability and sustained release ability. In addition, live/dead fluorescent staining and the CCK-8 test confirmed that LIP@ TMP had good biocompatibility. Moreover, middle cerebral artery occlusion (MCAO) rat model experiments further demonstrated that LIP@TMP could significantly alleviate cerebral ischemia and reperfusion injury by improving neurological scores, reducing cerebral infarct volume, promoting neurogenesis, inhibiting inflammation, and reducing tissue damage. In addition, LIP@TMP enhanced neuronal marker doublecortin (DCX) and neuronal nuclei (NEUN), inhibited inflammatory factors (TNF-α and IL-1β), and reduced apoptosis signal molecules (TUNEL and caspase-3). The findings of this study suggested that the prepared LIP@TMP had tremendous potential for the treatment of cerebral ischemia.

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