The purpose of the work was to determine the concentrations of mineral elements in subchondral bone and synovial fluid of patients with degenerative dystrophic knee joint diseases accompanied by articular surface defects. Subchondral bone and synovial fluid were used as a study material. Analysis of mineral content indices included the determination of calcium, phosphate, magnesium and chlorides concentrations by spectrophotometry. It was shown that the indices of basic electrolytes, i.e. of calcium and phosphate ions, in bone and synovial fluid did not correlate with each other and changed in different directions. Calcium content decreased in the bone while phosphate ions - in synovial fluid.

Федеральное государственное бюджетное учреждение «Российский научный центр "Восстановительная травматология и ортопедия" им. акад. Г.А. Илизарова» Министерства здравоохранения Российской Федерации, г. Курган, Россия Comparative biochemical analysis of synovial fluid constituents in infected cases following total knee replacement

Исследованы показатели электролитов и активность ферментов костного ремоделирования, концентрации продуктов перекисного окисления липидов и активность каталазы в синовиальной жидкости человека в норме. Оценены значения данных тестов в суставной среде коленного и локтевого сустава, достоверность различий по полу и возрасту человека.Ключевые слова: электролиты, кислая фосфатаза, щелочная фосфатаза, перекисное окисление липидов, окислительная модификация белков, синовиальная жидкость, сустав.

Chronic osteomyelitis in long tubular bones is generally aggravated by metabolic imbalance in patients’ organisms affecting mineral metabolism. It is critically important that the extent of this imbalance should be determined prior to surgical intervention to choose optimal methodology, proper monitoring of recovery and adequate prognostication of final results. With this in mind a comparative assessment has been carried out to shed light on the intensity of mineral metabolism in the blood serum of patients suffering from osteomyelitis in their shoulders and crura. Dynamics of how indices of electrolytes and activity of acidic and alkaline phosphatases vary have been studied in blood serum samples in 24 patients, 18 patients suffering from osteomyelitis in crus bones and 6 - in shoulders. In addition, correlations between total calcium, inorganic phosphate and phosphatase index have been computed. The mineral metabolism indices which were exhibited by the patients having shoulder osteomyelitis and by the patients with crus osteomyelitis are statistically meaningful differences prior to surgery and 2-3 days after surgery. 21 days after surgical intervention the activity of osseous remodeling enzymes, content of total calcium and phosphates as well as the correlations with the phosphatase index show no significant differences. These specific features of mineral metabolism turn out to level out in the process of treating chronic osteomyelitis in upper and lower limbs with transosseous compression-distraction osteosynthes. It has been the first study aimed at discovering comparative characteristics of mineral metabolism in patients with shoulder osteomyelitis and the ones with crus osteomyelitis at different times following surgical intervention. The study looks into correlations between the electrolytes and the mineralization index and shows specific traits exhibited by patients who suffering from osteomyelitis in shoulder and crus went through reparative regeneration at different periods of treatment.