O wing to high (46 %) dependency of the agroindustrial complex of the Russian Federation on livestock production, increasing the livestock preservation and productivity rates is a priority task of modern livestock breeding (Baranikov et al., 2017;Donnik et al., 2015). The chemotherapeutic medicinal products used at livestock breeding farms do not always have the desired effects, as they have many side effects. The use of most chemotherapeutic products results in decreasing the quality of the livestock product. Therefore, an alternative way of resolving these problems, according to most domestic (Nozdrin et al., 2017;Teplyakova, 2018) and foreign researchers (Morgan et al.
The current literature is not comprehensive concerning the influence of Bacillus subtilis on lipid metabolism, egg chemical characteristics, and intestinal microbiota of Japanese quail. The aim of this study was to evaluate the effects of a Bacillus subtilis strain on yolk quality, gut bacterial populations, and total cholesterol and triglyceride concentrations in the plasma of organically farmed Pharaon quail layers. Forty-five-day-old female quails were randomly distributed into four groups of 10 birds each: a no-treatment control group and three test groups receiving powder of Bacillus subtilis DSM 32424 at a minimum rate of 1 × 106 colony forming units per gram, dissolved in drinking water at doses of 50, 75 and 100 mg per kg body weight, daily during 30 days. The use of the probiotic at doses of 50 and 100 mg resulted in significantly reduced faecal staphylococci count on day 30 (P<0.05), while no significant changes were detected in lactobacilli or coliform bacteria. This study gives the first evidence of the influence of Bacillus subtilis on acid value and carotenoid levels in egg yolks laid by quails. Carotenoids were significantly elevated in the 75 mg group on day 30 (P<0.05), but significantly decreased in the 50 and 100 mg groups (P<0.05). Acidity, triglyceride and total cholesterol concentrations in quail serum were not affected significantly by the treatment throughout the study, though their values were reduced. The inclusion of Bacillus subtilis DSM 32424 in drinking water for laying Pharaon quails inhibited faecal staphylococci proliferation and enhanced yolk carotenoid content. Therefore, it can be proposed that including this probiotic in laying quail diets may have beneficial outcomes for both layers and the second generation.
The authors investigated the effect of probiotic Vetom 1 based on Bacillus subtilis strain DSM 32424 with concentration of active component 106 CFU/g on cumulative indices of Japanese quail egg-laying. The specimen was applied in doses of 50, 75 and 100 mg/kg of the live weight of quails. The probiotic Vetom 1 increases the number of ovulating birds. The maximum increase in this indicator was observed when using Vetom 1 dosed as 50 mg/kg of the body weight. The egg mass variability depends completely on the dose. Being used in a dose of 50 mg/kg weight, a higher variability of the egg mass was observed. This pattern has not been recorded in the maximum studied dose. The cumulative function in terms of eggs in unit and mass equivalents is slightly expressed in the initial period when applying Vetom 1. In the final period of specimen applying, the highest accumulation is observed when using Vetom 1 in a dose of 50 mg/kg of mass. When applying Vetom 1 in doses of 75 and 100 mg/kg, a direct correlation between ovulation frequency and egg weight was observed only in the initial period of the specimen applying, and the dose of 50 mg/kg - only for the eighth day of it.
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