н а у ч н о п р а к т и ч е с к и й ж у р н а л ов о щ и р о с с и и № 1 (3 9) 2 0 1 8 I S S N 2 0 7 2-9 1 4 6 (P r i n t) [ 12 ] СЕЛЕКЦИЯ И СЕМЕНОВОДСТВО СЕЛЬСКОХОЗЯЙСТВЕННЫХ РАСТЕНИЙ Фотев Ю.В. 1-кандидат с.-х. наук, с.н.с. Артемьева А.М. 2-кандидат с.-х. наук, зав. отделом генетических ресурсов овощных и бахчевых культур Фатеев Д.А. 2-аспирант, м.н.с. Наумова Н.Б. 3-с.н.с, кандидат биол. наук Бугровская Г.А. 3-ведущий инженер Белоусова В.П. 1-н.с. Кукушкина Т.А. 1-с.н.с.

The article presents the results of studying the biochemical composition of the large worldwide vegetable crops collection of the Brassicaceae family, stored at the All-Russian N.I. Vavilov Institute of Plant Genetic Resources (VIR), St. Petersburg, Russia, consisting of 7303 accessions of different status (approximately 50% unique accessions) from 98 countries. Studied vegetable brassicas collection includes 1 representatives of all botanical varieties, agro-ecological groups and cultivar types of the species Brassica oleracea L. and related wild Mediterranean species, leafy and root vegetable crops B. rapa L., leafy and root vegetable accessions of Indian mustard B. juncea Czern. subsp. integrifolia (H. West) Tell. and subsp. foliosa L. H. Bailey, radish and small radish Raphanus sativus L., garden cress Lepidium sativum L., salad rocket Eruca sativa L.The application of complex analysis for the study of nutrient and biologically active compounds of economically important crops from six species of Brassicaceae family, which determine the quality of vegetables and feeds, also the search within each crop for sources of valuable biochemical traits for breeding use has been presented. The broadest variability of all studied traits between and within crops was revealed, the limits of natural variability were determined.The average values of the studied traits in six species within the family varied to varying degrees: they were similar in the studied species in terms of dry matter content %, protein, total acidity; differed between species to a moderate degree in terms of the content of ascorbic acid, carotene, and the amount of volatile phenolic compounds. The species differed to a very high degree from each other in terms of the average content of sugars (total, monosaccharides, etc.), carotenoids, β-carotene, chlorophylls a and b, anthocyanins, free amino acids, and free fatty acids. Studied crops, belongs toBrassica oleracea, has the highest total sugar content among the all studied crops. B. rapa leafy crops has the highest content of phenolic compounds; B. juncea -β-carotene and chlorophylls; Raphanus sativus -carotenes and anthocyanins; Lepidium sativum -the highest content of protein, total acidity, free amino acids; Eruca sativa -carotenoids and free fatty acids. 2 The maximum dry matter content was detected in the forage turnip B. rapa and Brussels sprout (B. oleracea), protein in leafy B. rapa crops and cauliflower (B. oleracea), total sugars and ascorbic acid in head cabbage (B. oleracea), carotenoids in L. sativum and E. sativa, carotenes, including β-carotene, chlorophylls, phenolic compounds, free fatty acids in B. rapa, anthocyanins in R. sativus, free amino acids in B. rapa and R. sativus.Among the studied variety types of each crop, sources of nutrients and biologically active substances were identified, including types with an optimal biochemical compounds composition for a balanced the human nutrition, which are proposed to be useful in breeding of the new cultivars for healthy diet and medical a...

Background. Broccoli is an early-ripening vegetable crop that contains many biologically active compounds and mineral elements. According to the Genesys database, the global genebank collections contain no more than 465 different broccoli accessions. Fourteen cultivars and hybrids developed in Russia are registered in the State Register of the Russian Federation. The need to improve the assortment in a number of important breeding target areas (small habitus, non-spawning, bud size, disease resistance, etc.) requires the use of new effective techniques, including marker-assisted selection methods and association mapping. In this regard, it seems relevant to evaluate the VIR collection of broccoli using molecular genetic markers, which will provide new source material for breeding.Materials and methods. A molecular genetic study involved 39 broccoli cultivars and hybrid populations of different geographical origin, with various biological characteristics, and for various uses. For the analysis, 35 markers of microsatellite sequences specific to the Brassica L. genome were selected. PCR products were separated by electrophoresis on a 3% agarose gel.Results and conclusions. As a result, 110 polymorphic fragments were identified. In the studied loci, 3 to 7 alleles were pinpointed. The discriminating power of markers ranged from 0.75 to 0.96, and averaged 0.91; the average number of fragments per marker was 4.4. Ten unique alleles and 12 rare alleles (found in less than 8% of the samples) were observed in the studied accessions. On the other hand, the 201 bp allele of the locus BC65 was found in 95% of accessions, that is, it was almost common. All used markers have a sufficiently high diagnostic value and can be recommended for DNA identification in broccoli cultivars. An analysis of the genetic similarity of the collection accessions, carried out in the DarWin program using the Unweighted Neighbor-Joining method, made it possible to establish four closely related clusters.

Based on comparison of R2R3 Myb genes in Solanaceae (S. lycopersicum: Ant1, Ant2, S. melongena: Myb1, C. annuum: Myb113-like1 and Myb113-like2) and Brassicaceae crops, a search for orthologous sequences was The sequences encoding Myb114 TF in Brassica oleracea and Brassica rapa was found to be the closest in a nucleotide structure to the previously investigated genes in Solanaceae. Тhе polymorphism in the promoter region of the Capsicum annuum Myb113-like1 gene that regulates anthocyanin biosynthesis: an additional 148 bp repeat and 2(1) bp insertion in the forms with impaired anthocyanin synthesis in fruits was studied. A relationship between the presence of an insert in the promoter (Myb113-like1pr+148) and polymorphisms in the exon regions of Myb113-like1delTand Myb113-like2C/Аgenes associated with impaired anthocyanin synthesis was established. A number of polymorphisms of the Myb114 gene in vegetable crops of the cabbage family (Brassica oleracea, Brassica rapa), which closely correlate with high/low accumulation of anthocyanins in leaves, were identified. In B. oleracea, SNPs that lead to the replacement of two amino acids located in the region of DNAbinding domains were found, which leads to a change in the efficiency of binding of this transcription factor and the promoters of structural biosynthesis genes. A protein sequence encoded by the Myb114 gene in accessions of the leafy turnip (B. rapa) with a high accumulation of anthocyanins in the leaves differed from the accessions without anthocyanin accumulation in the leaves by five amino acids, while the regions of DNA-binding domains were the same in the forms with various anthocyanin accumulation.

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