We report on the lactate biosensor with linear calibration range from 0.5 to 100 mM, which encircles possible levels of this metabolite concentration in both human sweat and blood. The linear calibration range at high analyte concentrations, which exceeds the Michaelis constant of lactate oxidase by several orders of magnitude, is provided by an additional perfluorosulfonated ionomer diffusion membrane. In contrast to the known lactate biosensors, which retain their response within less than a couple of hours, the reported system displays 100% response for dozens of hours even upon high analyte concentrations. The biosensors with an additional diffusion-limiting membrane have been validated for lactate detection both in human blood serum and in undiluted human sweat shortly after its secretion. Both linear response in the entire range of blood and sweat lactate concentrations and ultrahigh operational stability would provide the use of the elaborated biosensor in wearable devices for the monitoring of hypoxia.

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Abstract. The article suggests a new look at trading platforms, which is called "metrics." Metrics are a way to look at the point of sale in a large part from the buyer's side. The buyer enters the store and make buying decision based on those factors that the seller often does not consider, or considers in part, because "does not see" them, since he is not a buyer. The article proposes the classification of retailers, metrics and a methodology for their determination, presents the results of an audit of retailers in St. Petersburg on the proposed methodology.

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