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Abstract. The study is aimed at analyzing the oxidoreductase activity of seeds and seedlings of soybean infected with a fungal infection of Septoria glycines Hemmi. Research Methods. The object of the study was seeds collected from soybean plants (Glycine max (L.) Merr) of the Lydia cultivar grown on meadow chernozem-like soil of the experimental field of the All-Russian Research Institute of Soybean in s. Garden of the Amur Region in 2019. The content of malondialdehyde was determined using thiobarbituric acid. The activity of superoxide dismutase and catalase was determined spectrophotometrically, and the activity of peroxidase and polyphenol oxidase was determined by the colorimetric method. Electrophoretic spectra of the studied enzymes were detected by electrophoresis on columns of a 7.5% polyacrylamide gel. Detection on a gel of zones with enzymatic activity was carried out by appropriate histochemical methods. Results. As a result of studies, it was found that on the 10th day the infection of the seedlings was 8.75 %. In this case, under the influence of Septoria glycines Hemmi, a slowdown in growth processes was observed, the level of malondialdehyde increased, which indicates an increase in oxidative processes. The length of seedlings infected with septoria was 2.7 % less compared to uninfected ones; the mass of infected seedlings decreased by 0.15 g. When soybean infection with Septoria was detected, soybean seeds and seedlings responded, expressed in a change in the specific activity of the studied enzymes and their rearrangement multiple forms. Reduction of oxidative stress in the seeds occurred due to an increase in the activity of superoxide dismutase, peroxidase and polyphenol oxidase, for seedlings – due to an increase in the activity of peroxidase only. Scientific novelty. It has been established that superoxide dismutase in soybean seeds has a high responsiveness to the pathogen. This is expressed in increased specific activity and significant polymorphism of the enzyme, which allows it to be used as a molecular marker for increasing soy resistance to the pathogen.

The results of the analysis of changes in biochemical parameters of soybeans depending on growing conditions are presented. The object of the study was samples of soybean (Glycine max (L.) Merr) of the Lydia variety and wild soybean (Glycine soja Sieb. & Zucc.) forms of KA-1344 grown on natural soils (control and with a high content of heavy metals). The analysis was carried out in leaves, stems, roots, flowers and seeds of cultivated and wild soybeans in the phase of the first triple leaf, flowering and fruiting. The content of malonic dialdehyde and the specific activity of acid phosphatase were determined by spectrophotometric method, the activity of peroxidase – by colorimetric method, the content of zinc, copper and lead – by atomic absorption. Electrophoretic spectra of acid phosphatase were detected by electrophoresis on columns of 7.5% polyacrylamide gel. Identification of zones with enzymatic activity on the gel was carried out by appropriate histochemical methods. It has been established that the cultivation of soybeans on soil with a high content of zinc, copper and lead leads to their accumulation in the organs of soybeans. The highest content of the studied metals is found in the roots. Growing soybeans on soil with a high content of zinc, copper and lead led to an increase in the specific activity of peroxidase in its organs. At the same time, the content of malonic dialdehyde in cultivated soybeans significantly increased only in stems during the phase of the first triple leaf and in roots during the flowering phase, and in wild soybeans in leaves, stems and roots during the phase of the first triple leaf and in stems during the flowering phase. It has been revealed that the flowers have the maximum specific activity of acid phosphatase. Cultivated soybeans, under conditions of increased zinc, copper and lead content in the soil, were characterized by an increase in the specific activity of acid phosphatase and the appearance of new multiple forms. For wild soybeans, in general, there was a decrease in the specific activity of acid phosphatase and an increase in the number of multiple forms of the enzyme.