Yeast production is one of the cost-effective ways to solve the problem of protein deficiency in food and feed. They are grown on nutrient media of various origins and compositions. Perennial non-traditional plants are promising in this regard. One of the industrial crops widely used in biotechnology, agriculture, medicine, food industry, etc., is Jerusalem artichoke (Helianthus tuberosus), including the variety Interest. The authors determined the chemical composition of Jerusalem artichoke tubers in the air-dry state. Data analysis showed that Jerusalem artichoke tubers have a rich composition and are a suitable raw material for nutrient media preparation. The nutrient medium was prepared by heat treatment. Yeast cultivation was carried out at a temperature of 37°C, pH 4.5. The yeast strain Saccharomyces cerevisiae was used as a producer of unicellular protein. The main component of the nutrient medium is Jerusalem artichoke. During cultivation, the dynamics of changes in the number of cells and accumulation of their biomass were studied. The increase in yeast biomass was 21 g/l, and the number of CFU was 450 million/ml. Active assimilation of sugars by yeast occurs from the first hour after the start of cultivation and stops by the ninth hour. Incomplete assimilation of sugars is associated with the accumulation of metabolic products in the environment, which significantly affects their activity. The obtained research results indicate the balance of the substrate in terms of nutritional components, as well as the expediency of its use for the cultivation of different types of yeast.
It has been found that the cup plant green mass is a promising substrate to culture yeasts, including Saccharomyces cerevisiae. A comparative study of efficiency to use the dried and intact green mass of this plant as a component of the medium for yeast cultivation was carried out. In the Republic of Ossetia-Alania, S. perfoliatum is a perennial, high-yield introduced species, yielding up to 1500 cwt of green mass per hectare. Using the cup plant green mass in to grow yeasts is economically beneficial, due to its high nutrient content. Key words: yeasts, culture media, cup plant, hydrolysis, biomass Funding - The work was financed by the extra-budgetary fund of the Gorsky State Agrarian University.
Разработана технология производства овощного пюре из корнеклубней якона, успешно интродуцированного в Республику Северная Осетия - Алания. Изучая химические показатели корнеклубней якона, установлено, что они отличаются богатым химическим составом и содержат сухих веществ - 11,9 %, протеина - 1,88 %, жира - 0,21 %, клетчатки - 1,06 %, золы - 1,48 %, безазотистых экстрактивных веществ - 9,0 %, инулина - 9,0 %, кислотность - 0,22 %. Якон имеет богатый минеральный состав. В 1 кг корнеклубней якона содержится кальция - 0,4 г, фосфора - 0,3 г, железа - 56,1 мг, меди - 0,57 мг, цинка - 3,0 мг, марганца - 12,6 мг, кобальта - 0,03 мг, йода - 0,01 мг, селена - 0,041 мг. Установлено, что в пюре из якона содержание сухих веществ составляет 15,0 %, что соответствует стандартам, содержание жира достигает 4,5 %, а минеральных примесей - 0,003 %. Растительные и посторонние примеси не обнаружены. По внешнему виду пюре из корнеклубней якона представляет собой однородную пюреобразную массу. Запах пюре фруктовый, без постороннего запаха. Вкус сладковатый, полный, насыщенный с приятным послевкусием, без постороннего вкуса. Цвет пюре однородный по всей массе: от желтого до светло-коричневого. Потемнение поверхностного слоя отсутствует. Особую ценность якону придает наличие в его составе инулина - природного полисахарида, на 95% состоящего из фруктозы, что позволяет корневые клубни якона и полученный продукт из него - пюре - рекомендовать как диетический продукт, в том числе людям, страдающим сахарным диабетом и ожирением. Установлено, что целесообразно использовать корневые клубни якона для производства овощного пюре. Пюре из корнеклубней якона по физико-химическим и органолептическим показателям соответствует требованиям стандартов. A technology to produce yacon pips-besed mashed vegetables successfully introduced to the Republic of North Ossetia - Alania is developed. Studying the chemical parameters of yacon pips, it was found that they differ in rich chemical composition and contain dry matter - 11.9 %, protein - 1.88 %, fat - 0.21 %, fiber - 1.06 %, ash - 1.48 %, nitrogen-free extractives - 9.0 %, inulin - 9.0 %, acidity - 0.22 %. Yacon has the rich mineral composition. 1 kg of yacon pips contains calcium - 0.4 g, phosphorus - 0.3 g, iron - 56.1 mg, copper - 0.57 mg, zinc - 3.0 mg, manganese - 12.6 mg, cobalt - 0.03 mg, iodine - 0.01 mg, selenium - 0.041 mg. It was found that mashed yacon contains 15.0 % of dry matter, which meets standards, the fat content reaches 4.5 %, and mineral admixtures - 0.003 %. No plant or extraneous matter was detected. In appearance, the yacon pips-based mash is a homogeneous, mash-like mass. The mash smell is fruity, free of any foreign smell. The taste is sweet, full, rich, with a pleasant aftertaste, free of foreign taste. The color of the mash is homogeneous throughout the whole mass from yellow to light brown. There is no darkening of the surface layer. The special value of yacon is due to inulin in its composition - natural polysaccharide, consists of 95 % fructose, which allows the yacon pips and the resulting product - mash, to be recommended as a dietary product, including people suffering from diabetes and obesity. It was found that it is advisable to use yacon pips for the production of mashed vegetables. Yacon pips-based mash meets standards in physico-chemical and organoleptic parameters.
Авторами установлено, что корнеклубни якона могут использоваться эффективно в качестве компонента питательной среды для культивирования дрожжей. Выявлено, что добавление мальтозы в питательную среду с яконом не оказывает существенного влияния на накопление биомассы дрожжей Rhodotorula glutinis ВКПМ Y-3469 и Pichia kluyveri ВКПМ Y-4343 селекции НИИ биотехнологии Горского ГАУ. The authors found that yacon pips can be effectively used as a culture medium constituent to cultivate yeasts. It was found that the maltose supplements to the culture medium with yacon do not significantly affect the accumulation of yeast biomass Rhodotorula glutinis VKPM Y-3469, Pichia kluyveri VKPM Y-4343 selected by the Research Institute of Biotechnology, Gorsky State Agrarian University.
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