Étude de l'activité antimicrobienne des matériaux de matrice imprégnés d'antibiotiques pour la reconstruction des défauts du tissu osseuxIntroduction. À ce jour, une nouvelle tendance se poursuit en médecine, qui implique la combinaison de matériaux fibreux avec des agents thérapeutiques tels que les systèmes d'administration de médicaments et les cellules vivantes. À cet égard, la chirurgie reconstructive a formé une nouvelle direction -l'ingénierie tissulaire, qui vise à restaurer les fonctions biologiques, c'est-à-dire à régénérer les tissus, pas seulement à les remplacer par du matériel synthétique.Objectif. Étudier la capacité d'absorption d'antibiotiques des matrices non tissées tridimensionnelles pour la reconstruction des défauts osseux, créées en

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Background:The major challenge in tissue engineering is to optimize cell isolation, multiplication and differentiation, as well as to construct the matrices or delivery systems thereby promoting the maintenance and coordination of three-dimensional tissue regeneration. One of the important criteria that should be considered when constructing the matrix is its ability to form an optimal scaffold for the transplantation of the cell substrates. Aim of the Study:To experimentally assess the nature of the development of the collagen scaffold during all the periods of subcutaneous implantation of the biopolymer fibrous matrix. Materials and Methods:The study included 20 laboratory animals (rabbits) divided into 2 groups: 10 animals of Group I underwent surgery involving the creation of a subcutaneous pocket and suturing; 10 animals of Group II underwent subcutaneous implantation of the biopolymer matrix into the back area between the shoulder blades. Nine segments were used for the study, namely 1 centrally located segment, 4 segments of the paracentral zone, 4 segments of the peripheral zone. Statistical analysis of the results was conducted using software programs Microsoft Excel and Statistica 5.5 (Multiple Regression) by means of the methods of variation statistics, correlation. Results:The results obtained indicated the absence of both acute and chronic reactive inflammatory infiltration, as well as both acute and chronic foreign-body response to the implant at the implantation site. Conclusions:The fibrous matrix constructed by us creates a kind of the bridge for tissue ingrowth and the formation of a three-dimensional collagen matrix. BackgroundBiopolymers are currently attracting increased attention in medicine and bioengineering. In tissue engineering, the materials for creating bioimplants must meet the following requirements: they must provide tissue engineered and microengineered constructs with the characteristics of living tissues, namely the ability to self-repair, as well as to change the structure and properties in response to the environmental factors [1]. The major challenge in tissue engineering is to optimize cell isolation, multiplication

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Introduction. Pain is a signal to any aggression that leads to cellular damage and requires a defensive response. Uncontrolled acute perioperative visceral pain can lead to the development of pain chronicity. By studying the characteristics of chronic pain, some scientists have identified relationships with single-nucleotide polymorphisms of the beta2-adrenergic receptor (ADRB2) gene. Purpose - to study the dependence of pain expression in the postoperative period in children on the polymorphism of the molecular structure of the ADRB2 receptor. Materials and methods. The study involved 42 children (20 boys and 22 girls) aged 7 to 18 years who were treated in the surgical department in 2020-2022 for acute appendicitis and peritonitis. Results. Based on the results of examining all the subjects under study, 13 children had the Arg16Gly polymorphism, 15 children had the Arg16Gly polymorphic variant, and 14 children were diagnosed with the homomorphic Gly16Gly polymorphism in the ADRB2. The data obtained confirmed the trend of the preliminary analysis and proved better body response to pain relief and reduced pain intensity in individuals with the Arg16Arg variant of the ADRB2. Analysis of the dependence between the polymorphism of the ADRB2 molecular structure and Visual Analogue Scale (VAS) scores in children in the postoperative period proved that the presence of Arg in the receptor phenotype had a strong negative correlation with the VAS score on discharge day (r=-0.822, p<0.001), while the presence of Gly in the receptor phenotype had a strong positive correlation with the Visual Analogue Scale score on discharge day (r=0.814, p<0.001). In regression analysis, the presence of Gly in the receptor phenotype was associated with a 1.917-fold increase in the VAS score at hospital discharge (OR: 1.917; 95% CI: 1.448-2.385; р<0.001). Conclusions. The presence of the homomorphic Arg16Arg variant of the ADRB2 in children who underwent anterior abdominal wall surgery was accompanied by rapid response to analgesics. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.