Abstract. The current study is a review of characteristics, production, physiological properties and application of xylooligosaccharides (XOS). XOS are the carbohydrates, their molecules are built from xylose residues linked mainly by в-(1→ 4)-glycoside bonds. Xylan is important for plant cell walls and is widely spread component in agricultural by-products. XOS are products of xylan hydrolytic degradation, and exhibiting the high prebiotic potential. The XOS preparation of wheat and rye bran stimulated the cells accumulation -1,4•10 10 CFU/cm 3 of L. аcidophilus and 9,2•10 10 CFU/cm 3 of В. bifidum. A difference in XOS molecules branching causes a wide range of their physiological properties: antioxidant, immunomodulation, antimicrobial, anti-inflammatory, anticarcinogenic. XOS can reduce high cholesterol level and triglycerides in blood plasma. XOS application reviewed in this article opens new perspectives on its potential use for human consumption. The rich sources of xylan are wheat, rye and barley bran, rice husk, wheat straw, corncobs, cotton stalk. Industrial way of XOS production includes chemical or enzymatic hydrolysis with following purification. Chemical methods are based on hydrothermal pretreatment and acidic or alkali extraction. Obtained oligosaccharides have a wide range of polymerization degree (DP) from 2 to 20. Enzymatic methods include fermentation with xylanase that allow controlling the XOS accumulation with certain DP. The different chromatographic purification after hydrolysis is used for analytical purposes. There are anion-exchange, size-exclusion, affinity, size-exclusion high-performance liquid chromatography. In addition, biomethods are preferred for XOS used in food, because such preparations do not contain monosaccharides and furfural as contaminants. XOS are stable in a wide range of temperature and pH, justifying the development of new synbiotics generation. Most widely XOS are used in production of functional products and pharmaceutical preparations. But they are also applied in cosmetic, agricultural and mixed feed industries.Keywords Анотація. Ця стаття є критичним оглядом ксилоолігосахаридів (КОС), їхньої характеристики, виробництва, фізіологічних властивостей і застосування. Ксилан важливий для клітинних стінок рослин і поширений в сільськогос-подарській сировині. КОС -це продукти гідролітичної деградації ксилана, володіють високим пребіотичним потенціа-лом. Відмінності в розгалуженні молекул КОС пояснюють широкий спектр їхніх фізіологічних властивостей: антиок-сидантні, імуномодулювальні, протимікробні, протизапальні, антиканцерогені та інші. Застосування КОС, розглянуте в цій статті, відкриває нові перспективи їхнього потенційного використання. Багатими джерелами ксилана є пшенич-ні, житні і ячмінні висівки, рисове лушпиння, пшенична солома, кукурудзяні качани, стебла бавовни. Промисловий спосіб виробництва КОС включає хімічний або ферментативний гідроліз із подальшим очищенням. Хімічні методи, засновані на гідротермічній попередній обробці і кислотній або лужній екстрак...

. In the article the conditions of enzymatic hydrolysis of fat fraction of waste from production of hydrogenated fat by the lipase Rhizopus japonicus are considered, namely, the influence of pH of the medium (pH-optimum, pH-stability) and temperature (thermal optimum, thermal stability). The scope of applications of lipases in various branches of the national economy, including for utilization of numerous fatty waste and by-products of oil and fat industry, is disclosed. The main reasons of biotechnological potential of microbial lipases are considered. Objects of research were the lipase Rhizopus japonicus and waste from the demetallization stage of the hydrogenated fat production. Detected, that the optimum pH value for Rhizopus japonicus lipase is 7.0, reducing the pH of the medium from the optimum to pH 6.0 is accompanied by a decrease in activity by 30%, and an increase from 7.0 to 9.0 – decrease by 20%. The maximum activity of the enzyme is observed in the region of physiological values of the temperature. It has been established that the lipase optimal temperature is 40°C. The results of the Rhizopus japonicus lipase stability study showed that incubation of the enzyme at pH 2.5 resulted in a complete loss of lipolytic activity after 30 minutes, and at alkaline pH, the enzyme was more stable. Incubation of lipase Rhizopus japonicus for 30 min at pH 9.0 leads to loss of lipolytic activity by 25% of the maximum, and total loss of activity occurs after 2.5 h. The study of pH-stability of Rhizopus japonicus lipase at an optimal pH of 7 showed that after 60 min of incubation, the enzyme lost 15% of the lipolitical activity, and after 60 min – 50%. Complete loss of Rhizopus japonicus lipase activity at pH 7.0 takes place after 150 minutes of incubation. The results of the study of thermal stability of lipase showed that at a temperature of 40°C and 60°C, the lipase activity remained rather stable for 50 minutes and completely lost after 150 minutes of incubation. At 80°C and 100°C, lipase activity was lost after 40 minutes and 50 minutes of incubation, respectively. The results of the study indicate the prospect of enzymatic hydrolysis of fat fraction of waste by Rhizopus japonicus lipase. The results obtained should be used to improve the processing technology of waste oil and fat industry food and processing industries.

In order to increase the efficiency of composting the organic component of solid municipal waste as a highly effective biotechnological method, and to compare the characteristics of the processes, it is suggested to use soil as an inoculum, as a microbiological additive - an extract from the soil. The original compost mixture is a multicomponent system, the decomposition and transformation of which depends on the functioning of a microorganisms complex, in particular, fungal and bacterial microflora. Since the main component of the organic fraction is cellulose, it is expedient, along with the definition of the total number of the microflora bacterial and fungal components, to determine the number of cellulose-decomposing microorganisms. An estimation of the change in the compost mixtures’ microbial population has been made, which shows that bacteria dominate over fungi in compost mixtures. A similar microbial complex is observed in soils. The article presents the results of the study of the soil microbiocenosis qualitative and quantitative composition in order to use it as an inoculum in the process of composting the organic part of solid municipal waste. The influence of microbiological additive on the process of the organic waste composting for acceleration in mesophilic and thermophilic temperature regimes with controlled parameters was studied. The results of the conducted studies allow us to conclude that the organic waste composting with the microbiological additive is appropriate, both in the case of thermophilic and in the case of mesophilic regimes. The period of the compost maturation with the use of a microbiological additive is 6 weeks. It was shown that the microbiological complex accelerates the process of composting the organic component of solid municipal waste by 3.3 times for the thermophilic regime and by 2.1 times for the mesophilic conditions of composting process, which testifies to the efficiency of its use in the operation of the solid municipal waste processing in order to improve the general level of environmental safety.

Aerobic composting is one of the best available technologies for an integrated waste management system in terms of minimizing the anthropogenic impact on the environment, complying with the latest domestic and foreign developments, economic and practical acceptance of the technology. Organic production is rapidly spreading in the world as a holistic system of rational nature management that becomes the basis for the use of modern composting technologies for organic raw materials to obtain a quality product of processing. But composting is characterized by relatively low popularity in comparison with other methods of waste utilization due to a number of its disadvantages, such as a long production cycle and sometimes the unstable quality of a product obtained. Because of this, many studies in the field of solid waste recycling are devoted to methods of accelerating the composting process. This can be achieved in various ways, such as the development of high–performance composting machines and biotic changes (vermiculture, use of specialized crops and biocenoses of microorganisms), or abiotic (temperature, pH, etc.) parameters of the process.The article presents the results of studying the effect of mineral additives on organic waste composting processes, with the aim of its accelerating in mesophilic and thermophilic temperature regimes with controlled parameters. To improve the efficiency of the composting process and compare the features of the processes, the soil was used as a microbiological inoculum, and mineral salts solutions as a mineral additive. The results of the studies allow us to conclude that it is advisable to compost plant waste with a mineral additive, both in the thermophilic and the mesophilic mode. The compost ripening period, with a mineral additive used, is 6 weeks. It is shown that the mineral complex accelerates the composting of the organic constituent of municipal solid waste by 2.2 times in the thermophilic mode, and by 1.4 times under the mesophilic conditions of the composting process, which proves the effectiveness of its use in recycling municipal solid waste to increase the general level of environmental safety.

В пищевой промышленности лимонная кислота играет важную роль, оставляя вопросы утилизации отходов производства открытыми. При приготовлении кислоты используется больше сырья, чем получают готовой продукции на выходе, при этом образуется большое количество отходов, негативно влияющих на окружающую среду, среди них: фильтрат цитрата кальция, гипсовый шлам и мицелий гриба Aspergillus niger. Отходы мицелия повышает уровни опасности, загрязняя почвы и подземные воды в местах хранения. Целью данного исследования является поиск новых возможностей для вторичного использования отходов в промышленных и сельскохозяйственных сферах. Особое внимание уделено выделению хитина и хитозана из состава гриба Aspergillus niger и формирования хитиновых пленок с целью получения продукта со стабильным химическим составом независимо от качества исходной биомассы гриба, для эффективного управления процессом утилизации.