No abstract

Aim. To study the activity of myeloperoxidase in the peripheral blood of pregnant women in normal and thyroid diseases. Materials and methods. Cytochemical analysis was used to study the activity of myeloperoxidase in peripheral blood neutrophils of pregnant women with diffuse toxic goiter, as well as in women with the physiological course of pregnancy in a comparative aspect. Results. It was found that in women with a physiological course of pregnancy, the cytochemical parameters of myeloperoxidase in neutrophils were reduced only in the 1st trimester of pregnancy. In patients with diffuse toxic goiter, with severe thyrotoxicosis syndrome, the activity of the enzyme was significantly reduced during all periods of pregnancy. Apparently, these shifts of the enzyme, which is the main component of the protective system of blood phagocytes, are associated with their functional stress. Conclusion. Based on this opinion, it seems that blood neutrophils enter the system of thyroid hormone metabolism by their degradation. Apparently, this mechanism is activated with an increased content of hormones in the circulation, which occurs during pregnancy, and especially with concomitant diffuse toxic goiter with thyrotoxicosis syndrome and is aimed at preserving homeostasis necessary for normal fetal development.

16.12.2015) проведено исследование по определению его возможностей при установлении функциональ-ного состояния щитовидной железы (ЩЖ). Уста-новлено что активность фермента определятся в фолликулярных тиреоцитах и существенно меняется при различных патологических состо-яниях органа. В частности, в гистохимических препаратах ЩЖ больных диффузным токсическим зобом активность ТПО в стенке фолликулов и ме-жфолликулярных аденоматозных очагах высокая. Характерно наличие эпителиальных выпячиваний с выраженной активностью в периферических тиреоцитах. При заболеваниях ЩЖ в стадии эу-тиреоза активность фермента в фолликулярных тироцитах умеренная. Содержание фермента существенно снижено при макро-и микрофолли-кулярном коллоидном зобе и аутоиммунном ти-реоидите в стадии гипотиреоза. Делается вывод, что результаты предлагаемого гистохимического метода определения активности ТПО позволяют дополнить морфологическое заключение патоло-гоанатомов функциональным. Ключевые слова: щитовидная железа, ги-стохимия, тиреопероксидазаDeveloped at the Department of Histology DSMU histochemical method of detecting activity thyroperoxidase (TPO) (invention, foreground number 2015054203 from 16.12.2015) conducted a study to determine its capabilities when establishing a functional state of the thyroid gland (TG). It was found that the enzyme activity was determined in the follicular thyrocytes and varies considerably in various pathological conditions the organ. In particular, in histochemical preparations thyroid patients with diffuse toxic goiter TPO activity in the wall of the follicles and foci of adenomatous interfollicular high. It is characterized by epithelialprotrusion with marked activity in peripheral thyrocytes. In diseases of the thyroid gland in the stage euthyrosis enzyme activity in follicular thyrocytes moderate. The content of the enzyme significantly reduced by the macro and micro follicular colloid goiter and autoimmune thyroiditis in the stage of hypothyroidism was determined. It is concluded that the results of the proposed histochemical method for determining the activity of TPO allow pathologists to complement morphological conclusion functional.