Somatic embryogenesis (SE) is the development of embryo-like structures from somatic plant tissues. This process rarely can be observed in nature, but for many plant species, in vitro protocols are developed, which allow to obtain somatic embryos formation directly from tissues of plant explant or from the embryogenic callus. SE is widely used for plant propagation and transformation; therefore, the search for SE stimulators and revealing of the mechanisms of their functioning are very important for biotechnology. Among the SE regulators, proteins of the WOX family play significant roles. WOX (WUSCHEL-RELATED HOMEOBOX) is a homeodomain-containing transcription factor family. Different WOX genes function in different plant organs and tissues, maintaining meristem activity and regulating cell proliferation and differentiation. Recently, we have shown that transcription factor MtWOX9-1, belonging to the WOX family, can stimulate SE in the Medicago truncatula callus culture. In this research, transcriptomic analysis of highly embryogenic calli with MtWOX9-1 overexpression was performed in comparison to wildtype calli. It was shown that MtWOX9-1 overexpression led to the activation of several groups of genes, including genes related to cell division, tissue diff erentiation, and seed development. Enriched GO pathways included several groups related to histone methyltransferase activity as well as DNA methylation and chromatin binding, suggesting major epigenetic changes that occur in call overexpressing MtWOX9-1. Using Medicago Truncatula Gene Expression Atlas, we also iden tified a group of genes coding for transcription factors that were both coexpressed with MtWOX9-1 in different plant organs and differentially expressed in our samples. These genes are putative targets of MtWOX9-1, and they may act in the same pathway with this regulator during SE.
Two widespread weedy species of Urtica L. (Urticaceaea Juss.), common nettle (Urtica dioica L.) and annual nettle (U. urens L.), possess many useful properties, so any information on their distribution is of practical interest. Numerous scientific publications with open access were used to map the areas of distribution for these species in the Russian Federation. While mapping the spatial arrangement of these taxa, the data on the frequency of their occurrence in separate administrative subdivisions of the country were employed. Areas where occurrences of an individual nettle species are scored as "very often", "often", "usually" or "often" were blended into one territory characterized with the "often" occurrence: this is the optimum zone for the species in question. Similarly, the pessimum zone for the species was identified as a combined territory of the areas where the occurrence was marked as "very rarely", "rarely", "infrequently" or "sporadically". The pessimum zone included the areas located in the north of the range of species distribution: they are characterized by cooler and more humid environmental conditions than the optimum zone. The pessimum also incorporated the lands stretching in the southern part of the range: their environmental conditions are drier and warmer than those in both the optimum zone and the northern part of the pessimum zone. Therefore, plants of the same species, occurring in different parts of their area of distribution, are adapted to different hydrothermal conditions, which should be taken into account when selecting forms for further possible cultivation and use.
On the territory of Kazakhstan there are uranium deposits, many ofwhich are in mothballed since times of perestroika. Often, the mines are flooded and represent a “time-delay bomb”. Inside of mines various there are accumulated gases of both organic and inorganic nature, periodically thrown out and adversely affecting on the health of local populations. The aim of the study was the investigation of the state of the environment of Esilsky district of the Akmola region by common pollutants and chemicals. As the basic variable for the investigation of ambient air there was accepted the maximum one-time concentration of suspended substances, phenol, nitrogen dioxide, sulfur dioxide. The results were evaluated in relation to the MPC for the analyzed substance in the air according to maximal single MPC (MPCms) and daily average MPC (MPCda). The content of metals in the water was determined with the use of spectrophotometer PD-303S. Evaluation of the results was executed in relation of the MPC of substances in water, by means of the comparison with the requirements of Federal standards for drinking water, samples from drinking water sources. There were executed calculations of the overall index of water pollution (IWVgen), the index of water pollution by heavy metals (IWVhm). Chemical analysis of soil was carried out with the use of spectrophotometer PD- 303S (Japan), the photometer expert-003 “Ekoniks”. Evaluation of the results was carried out with the respect to the MPC in the soil, the toxicity of all components. Summarizing soil pollution index was evaluated for metals contained in the soil at the level of more than or equal to 1 MAC. The settlement Krasnogorskiy and the village of Kalachi were found to be characterized by a low level of air pollution, increased rigidity of drinking water exceeded the maximum permissible concentrations of copper by 3.45 times and chloride by 1.17 times in the soil cover.
The article reviews the concept and technology of pest risk assessment for the spread of quarantine species based on ecological and geographical analysis and ecological niche modeling. Using a quarantine species Ipomoea hederacea (L.) Jacq. as an example, we calculated the ecological requirements of the species. The main ecological factors limiting the spread of I. hederacea were determined and the ecological amplitudes of the species in relation to each factor limiting its distribution were quantitatively estimated. We identified ecologically suitable habitats and compiled a map of the ecological-geographical niche of the species using specially created environmental maps based on the obtained data of the ecological limits of the species. Using the map of the ecological-geographical niche, the I. hederacea distribution risk in the regions of the Russian Federation was estimated. Similar maps can be used for basis of quantitative as well as targeted risk assessment of penetration and establishment of harmful organisms. Obtained information will be useful to compile lists of quarantine species, estimate the potential areas of geographic distribution of pests into Russian Federation, and make decisions for introduction of effective phytosanitary measures to prevent the penetration of these invasive organisms. It can also be used as the basis for organizing and conducting phytosanitary monitoring in the Russian Federation.
The ecological and hygienic assessment of environmental areas adjacent to the zones of anthropogenic exposure in the cold season of the year. As the object of research there was chosen ecologically unfavorable Aral Sea region -settlement Aiteke bi in the - Kyzylorda region of the Republic of Kazakhstan. The aim of research was to identify chemical pollutants in the environment of the settlement of Aiteke Bi. During the execution of the study there was used a complex of modern certified ecological-hygienic, chemical-analytical and static methods of the analysis (photoionizing, electrochemical, optical, photometric, complexonometric, weight, titrimetric). Results of the study of ambient air showed the excess of the concentration of suspended particulate matter 1.78 MPC daily averages, the value of Air Pollution Index (API ) was low (API4= 1.3 c.u.). The quantitative chemical analysis of drinking water showed the presence of low concentrations of heavy metals: cadmium - 0,3MPC, nickel - 0.5MPC, iron - 0.4MPC, zinc - 0.6MPC, cobalt - 0.4MPC (water pollution index (WPI)= 0.2 cu), clean water is of quality class 2. There was revealed a low level of soil contamination (Zc =0.1 c.u.), however, observed in all samples there were noted excesses of sulfate MPC (by to 193.8 times) and chlorides (3.9 times). Concentrations of polluting chemicals discovered in the total territory of the settlement of Aiteke bi are related to the activity of more than twenty-one companies producing oxygen, building materials, metal plastic products, executing the exploration and production of minerals. Each out of the identified chemical impurities is widely used in manufacturing, thereby is accumulated in the environment and easily gets into the body with both food and water, and via air inhalation. There is observed a chronic intake of chemical contaminants.
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