임난영 1) ․이인옥 2) ․이은남 3) ․이경숙 4) ․조경숙 5) ․이선자 6) 강현숙 7) ․김금순 8) ․김종임 9) ․박원숙 10) ․이윤경 11) ․전미영 12) 1) 한양대학교 간호학과 교수, 2) 대한근관절건강학회 교육센터장, 3) 동아대학교 간호학과 교수 4) 강릉원주대학교 원주캠퍼스 간호학과 교수, 5) 경원대학교 간호학과 교수, 6) 서울대학교 보건대학원 명예교수 7) 경희대학교 간호과학대학 교수, 8) 서울대학교 간호대학 교수, 9) 충남대학교 간호대학 교수 10) 경희대학교 동서신의학병원 관절.류마티스센타 교육실장, 11) 적십자간호대학 조교수, 12) 건국대학교 간호학과 교수

To identify new active anti-wrinkle ingredients, this study investigated the anti-wrinkle effects of Schizandra chinensis Baillon fermented with Lactobacillus plantarum (SCF) by assessment of cytotoxicity of human dermal fibroblast, collagen biosynthesis, matrix metalloproteinase-I (MMP-1) inhibition and elastase inhibition. S. chinensis was fermented with L. rhamnosus for 1 day at 37 ℃. The cytotoxicity of SCF was evaluated by a cytopathic effect reduction method. Effects on collagen biosynthesis and matrix metalloproteinase-I (MMP-1) of SCF were evaluated by previous reported method using procollagen type-IC peptide EIA kit and Matrix Metalloproteinase-1 Biotrack activity Assay Kit, respectively. Elastase inhibition assay was conducted by reaction of enzyme and substrate using N-Suc-(Ala) 3 -nitroanilide as the substrate. As the results, SCF didn't show cytotoxicity against human dermal fibroblast at concentration of 100 µg/mL. Also, SCF was increased collagen synthesis and showed inhibitory effect of MMP-1 (p < 0.05). In the elastase inhibition assay, the IC 50 of SCF was 36.4 µg/mL. Therefore, our results indicated that SCF possesses anti-wrinkle effects and can be used practically for anti-wrinkle care of skin.

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