Anti-Wrinkle Effect of Schizandra chinensis Baillon Fermented with Lactobacillus plantarum

Abstract

To identify new active anti-wrinkle ingredients, this study investigated the anti-wrinkle effects of Schizandra chinensis Baillon fermented with Lactobacillus plantarum (SCF) by assessment of cytotoxicity of human dermal fibroblast, collagen biosynthesis, matrix metalloproteinase-I (MMP-1) inhibition and elastase inhibition. S. chinensis was fermented with L. rhamnosus for 1 day at 37 ℃. The cytotoxicity of SCF was evaluated by a cytopathic effect reduction method. Effects on collagen biosynthesis and matrix metalloproteinase-I (MMP-1) of SCF were evaluated by previous reported method using procollagen type-IC peptide EIA kit and Matrix Metalloproteinase-1 Biotrack activity Assay Kit, respectively. Elastase inhibition assay was conducted by reaction of enzyme and substrate using N-Suc-(Ala) 3 -nitroanilide as the substrate. As the results, SCF didn't show cytotoxicity against human dermal fibroblast at concentration of 100 µg/mL. Also, SCF was increased collagen synthesis and showed inhibitory effect of MMP-1 (p < 0.05). In the elastase inhibition assay, the IC 50 of SCF was 36.4 µg/mL. Therefore, our results indicated that SCF possesses anti-wrinkle effects and can be used practically for anti-wrinkle care of skin.

Study on Efficacy Evaluation of Deramal Bioactive Properties of the Prunus tomentosa Fruits Fermented with Lactobacillus acidphilus

“…한편, 유산균은 프로바이오틱스로 세계적으로 그 유효성이 평가되고 있다 (Kang, 2003) (Fijan, 2014 (Park & Kim., 2020;Lee et al, 2014;Yoo et al, 2019;Jeon et al, 2011;Jeong et al, 2019;Choi & Jung, 2017 (Yuan et al, 2015).…”