This study was performed to investigate the effects of Cheonggukjang added with Job's tears (CAJT) on antiobesity and lipid profiles on diet-induced obese rats. Male Sprague-Dawley rats were fed high-fat diet only (A, control), and high-fat diet with Cheonggukjang powder 10% (B), CAJT powder 10% (C), Cheonggukjang powder 20% (D), CAJT powder 20% (E) for 5 weeks. Body weight gains were similar in the experimental groups, but FER was significantly lower in the group E than group A (p<0.05), and adipose tissue weights in groups with Cheonggukjang and CAJT powder were similar to those in the group A. The lengths of small intestine were significantly longer in the groups B, C, D, and E than the group A (p<0.05). Hepatic concentrations of total cholesterol (TC) and triglyceride (TG) were similar to each other. Fecal weights were not significantly different among the groups. Fecal TC concentrations were similar in the experimental groups, but fecal TG concentrations were greatly increased in the groups B, D, and E (p<0.05). The serum TC, HDL-C and LDL-C levels varied slightly among the groups, but serum TG levels were decreased in the group D and E than in the group A (p<0.05). The AI and CRF were marginally decreased in the groups B, D, and E than in the group A (p<0.05). However, blood glucose levels were not significantly different among the groups. From the findings, it had shown that Job's tears Cheonggukjang could improve lipid profiles.

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This study was performed to develop an odor-reduced Korean traditional Cheonggukjang (fermented soybean paste) by adding Job's tears to Cheonggukjang made with soybean (control) only. The study included the determination of best addition percentage of Job's tears versus soybean, sensory evaluation, and aroma patterns and its components as well as proximate composition analyses of Cheonggukjang. Job's tears Cheonggukjang (CAJT) was prepared by inoculating Bacillus subtilis and fermented at 40 o C for 48 hours. Ratios of soybean : Job's tears for Job's tears Cheonggukjang were 1:1, 2:1, 3:1, and 4:1, respectively. In comparison of proximal composition, Job's tears Cheonggukjang was high in moisture and carbohydrates, but low in calorie, fat, protein, ash and amino nitrogen. The pH of CAJT was lower than those of control and conventional Cheonggukjang (CC) sold in the market, and the acidity was the lowest in CC. The color of Job's tears Cheonggukjang appeared the whiter and more yellowish, but less reddish in proportion to contents of Job's tears. The contents of viscous substances were higher in CAJT compared with those of control and CC. The contents of glutamic acid, the major components of viscous substances were lower in CAJT than in control and CC, and the contents of fructose higher in CAJT. The scores of sensory evaluation were the highest in Job's tears Cheonggukjang mixed with soybean : Job's tears＝4:1. The aroma pattern of CC analyzed was conspicuously dissimilar to the control and CAJT and also discriminated by electronic nose examination. The pyrazines, volatile compounds peculiar to Cheonggukjang, were found to be lower in CAJT by SPME-GC/MS assay. Additionally, the acetic acid, butanoic acid, and naphthalene causing off-flavor were identified in CC, but not in Job's tears Cheonggukjang. The strength of odor through sensory evaluation was by far the lowest in CAJT among the groups. From the findings, it had shown that Job's tears Cheonggukjang would be produced successfully when the ratio of 4:1 (soybean : Job's tears) is employed. Also, it was proved that appropriate ratio of Job's tears addition makes Cheonggukjang odor-reduced and well accepted by people.