[36] Magnesium transport in eukaryotic and prokaryotic cells using magnesium-28 ion

Hypoxia Induces an Increase in Intracellular Magnesium via Transient Receptor Potential Melastatin 7 (TRPM7) Channels in Rat Hippocampal Neurons in Vitro

“…Magnesium, the second most common intracellular cation and the most abundant intracellular divalent cation (32)(33)(34), has been shown to be neuroprotective in several experimental models of ischemic and excitotoxic brain injury (20,21). However, other studies have failed to detect a neuroprotective effect of magnesium (22,23).…”

“…It had been reported that an excess amount of intracellular Mg 2ϩ induced by hyperactivity of N-methyl-D-aspartate channels occasionally causes neuronal cell death as a result of inhibitory effects of Mg 2ϩ to mitochondrial activities (24). The change of [Mg 2ϩ ] i in OGD/CI may also affect the cellular function in which magnesium is required as follows: maintenance of the active conformation of macromolecules, regulation of lipid-and phosphoinositide-derived second messengers, regulation of transporters and ion channels (33,54), etc.…”

SitABCD Is the Alkaline Mn2+Transporter ofSalmonella entericaSerovar Typhimurium

“…Manganese(II) uptake and iron(II) uptake were determined by incubating cells in DT medium (0.4% tryptone peptone, 0.25% NaCl) with 54 Mn 2ϩ (Amersham) as used by Silver and colleagues (29), with duplicate or triplicate samples at each time point or concentration. Cells were then processed essentially as for Mg 2ϩ transport assays from this laboratory (13). Cells were filtered with 0.45-m-poresize filters (BA85; Schleicher and Schuell), rinsed twice with 5 ml of ice-cold DT medium, and counted in BioSafe II scintillation cocktail (Research Products International, Inc.) in a Beckman LS6500 liquid scintillation counter.…”

Cation Hexaammines Are Selective and Potent Inhibitors of the CorA Magnesium Transport System

“…Transport-63 Ni 2ϩ uptake was measured as described previously (9,21). MM1111 was grown overnight in LB broth containing 100 mM Mg 2ϩ .…”