4-<i>O</i>-(1-carboxyethyl)-<scp>l</scp>-rhamnose, a second unique acidic sugar found in an extracellular polysaccharide from <i>Butyrivibrio fibrisolvens</i> strain 49

Stack, Robert J.; Weisleder, David

doi:10.1042/bj2680281

Cited by 10 publications

(3 citation statements)

References 15 publications

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“…Presumably, the EP aids in this associ-ation and possibly hinders diffusion of the diverse extracellular enzymes such as xylanases (9), proteases (2), or esterases (10) made by B. fibrisolvens. The EP may also serve in a protective role since the presence of unusual sugars might make the EP more resistant to the action of glycanases (19,24). Regardless of the natural roles, the EPs of some B. fibrisolvens strains may have potential commercial applications on the basis of their rheological properties, as shown in this study.…”

Section: Discussionmentioning

confidence: 76%

“…EPs from several strains have been characterized on the basis of sugar composition (20). The compositional analysis of EPs produced by 37 strains of B. fibrisolvens showed that unusual sugars such as L-altrose (19,21), 4-O-(1-carboxyethyl)-D-galactose (23), and 4-O-(1-carboxyethyl)-L-rhamnose (24) are common constituents of the EPs. However, the rheological and functional properties of these EPs have not been studied.…”

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confidence: 99%

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Some chemical and physical properties of extracellular polysaccharides produced by Butyrivibrio fibrisolvens strains

Stack

Hespell

et al. 1991

Appl Environ Microbiol

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Most strains of Butyrivibriofibrisolvens are known to produce extracellular polysaccharides (EPs). However, the rheological and functional properties of these EPs have not been determined. Initially, 26 strains of Butyrivibrio were screened for EP yield and apparent viscosities of cell-free supernatants. Yields ranged from <1.0 to 16.3 mg per 100 mg of glucose added to the culture. Viscosities ranged from 0.71 to 5.44 mPa s. Five strains (CF2d, CF3, CF3a, CE51, and H1Ob) were chosen for further screening. The apparent viscosity of the EP from each of these strains decreased by only 50 to 60% when the shear rate was increased from 20 to 1,000 S-. Strain CE51 produced the EP having the highest solution viscosity. A detailed comparison of shear dependency of the EP from strain CF3 with xanthan gum showed that this EP was less shear sensitive than xanthan gum and, at a shear rate of 1,000 s-, more viscous. EPs from strains CF3 and HlOb were soluble over a wide range of pH (1 to 13) in 80% (vol/vol) ethanol-water or in 1% (wt/vol) salt solutions. The pH of 1% EP solutions was between 4.5 and 5.5. Addition of acid increased solution viscosities, whereas addition of base decreased viscosity. EPs from strains CF3, CE51, and HlOb displayed qualitatively similar infrared spectra. Calcium and sodium were the most abundant minerals in the three EPs. The amounts of magnesium, calcium, and iron varied considerably among the EPs, but the potassium contents remained relatively constant.

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Section: Discussionmentioning

confidence: 76%

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confidence: 99%

Some chemical and physical properties of extracellular polysaccharides produced by Butyrivibrio fibrisolvens strains

Stack

Hespell

et al. 1991

Appl Environ Microbiol

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“…An interesting observation was the presence of 4-O-(1-carboxyethyl)-rhamnose in the EPS of strain 20-63. This unusual acidic sugar was previously found only in the EPS of specific strains of another anaerobic gastrointestinal bacterium, Butyrivibriofibrisolvens (20,33). We have speculated that unusual sugars in the EPS of gastrointestinal bacteria may serve to protect the bacteria from glycanases and/or glycosidases found in the digestive tracts of animals (31).…”

Section: Discussionmentioning

confidence: 80%

Taxonomic relationships among strains of the anaerobic bacterium Bacteroides ruminicola determined by DNA and extracellular polysaccharide analysis

Mannarelli

Ericsson

Lee

et al. 1991

Appl Environ Microbiol

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DNA and extracellular polysaccharide (EPS) analyses were performed on 14 strains of Bacteroides ruminicola. The guanine-plus-cytosine (G+C) base contents, determined from the buoyant densities of chromosomal DNAs, showed a broad range of values, from 37.6 to 50.9 mol%. DNA hybridization showed generally low DNA relatedness among the strains. Seven strains formed two groups of closely related bacteria consisting of five (group 1) and two (group 2) strains, and another strain, E42g, showed moderate relatedness to group 1 strains. However, the remaining six strains were not related to any of the other strains. DNA reassociation indicates that the strains constitute a genetically diverse group representing as many as nine separate species. EPS analysis showed that the strains produced EPS with rather uniform sugar compositions, which did not correlate with strain relationships determined by DNA analysis. Four strains had EPS with acidic sugars or unknown compounds. The EPS of strain 20-63 contained the unusual acidic sugar 4-O-(1carboxyethyl)-rhamnose. This monosaccharide has been shown to occur in nature in only one other bacterial species.

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Butyrivibrio

Willems¹,

Collins²

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Bu.ty.ri.vib'ri.o. N.L. adj. butyricus butyric; L. v. vibro to vibrate; N.L. n. Vibrio that which vibrates, a bacterial genus name; N.L. masc. n. Butyrivibrio a butyric vibrio. Firmicutes / “Clostridia” / Clostridiales / “Lachnospiraceae” / Butyrivibrio Gram‐stain‐negative , but structurally Gram‐positive , non‐sporeforming , strictly anaerobic straight to curved rods , 0.3–0.8 × 1.0–5.0 µm, occurring singly or in chains or filaments that may be helical. Motile by monotrichous or lophotrichous , polar or subpolar flagella or nonmotile . Chemo‐organotrophic , fermentative metabolism with carbohydrates as the main substrates. Glucose or maltose are fermented with butyrate as a major end product . Under some conditions some strains may produce large amounts of lactate and little butyrate. On the basis of 16S rRNA gene sequences, Butyrivibrio belongs to cluster XIVa of the Clostridium subphylum of the Gram‐positive bacteria. It is not monophyletic: Butyrivibrio fibrisolvens strains form two distinct subgroups which correspond to different genera and Butyrivibrio crossotus occupies a separate position (Willems et al., 1996). Butyrivibrio hungatei belongs to the Butyrivibrio fibrisolvens subgroup that also comprises the type strain of Butyrivibrio fibrisolvens (Kopečný et al., 2003). According to the 16S rRNA phylogenetic analysis presented in the roadmap to this volume (Figure 1), the genus Butyrivibrio is a member of the family Lachnospiraceae , order Clostridiales , class Clostridia in the phylum Firmicutes . Isolated from the rumen of ruminants and in human, rabbit, and horse feces. DNA G + C content ( mol %): 36–45 mol% ( T m and HPLC). Type species : Butyrivibrio fibrisolvens Bryant and Small 1956b, 19 AL .

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4-O-(1-carboxyethyl)-l-rhamnose, a second unique acidic sugar found in an extracellular polysaccharide from Butyrivibrio fibrisolvens strain 49

Cited by 10 publications

References 15 publications

Some chemical and physical properties of extracellular polysaccharides produced by Butyrivibrio fibrisolvens strains

Some chemical and physical properties of extracellular polysaccharides produced by Butyrivibrio fibrisolvens strains

Taxonomic relationships among strains of the anaerobic bacterium Bacteroides ruminicola determined by DNA and extracellular polysaccharide analysis

Butyrivibrio

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