Taxonomic relationships among strains of the anaerobic bacterium Bacteroides ruminicola determined by DNA and extracellular polysaccharide analysis

Mannarelli, Bruno M.; Ericsson, Linda D.; Lee, D; Stack, Robert J.

doi:10.1128/aem.57.10.2975-2980.1991

Cited by 43 publications

(17 citation statements)

References 39 publications

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“…P. ruminicola 20-78, 20-63, 23, and 118B produced less than onethird the CMCase activity of B 1 4, and Western blots indicated that the other strains that produced CMCases did not react with B 1 4 CMCase antiserum. These results support the idea that P. ruminicola is a highly divergent species that needs further reclassification (2,11). Strains B 1 4, TC1-1, TF1-3, and TS1-5 could be a separate species.…”

Section: Discussionsupporting

confidence: 83%

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The cellular location of Prevotella ruminicola beta-1,4-D-endoglucanase and its occurrence in other strains of ruminal bacteria

Gardner

Wells

Russell

et al. 1995

Appl Environ Microbiol

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Prevotella ruminicola B 1 4, TC1-1, TF1-3, and TS1-5 all produced immunologically cross-reacting 88-and 82-kDa carboxymethyl cellulases (CMCases). P. ruminicola 23, 118B, 20-63, and 20-78 had much lower CMCase activities, and Western blots (immunoblots) showed no cross-reaction with the B 1 4 CMCase antiserum. Fibrobacter succinogenes S85 and Selenomonas ruminantium HD4 and D produced CMCases, but these enzymes were smaller and did not cross-react with the B 1 4 CMCase antiserum. The B 1 4 CMCase antiserum inhibited the B 1 4, TC1-1, TF1-3, and TS1-5 CMCase activities and agglutinated these cells, but it had no effect on the other strains or species. On the basis of these results, the B 1 4 CMCase is a strain-specific enzyme that is located on the outside surface of the cells. P. ruminicola B 1 4 cultures, grown on sucrose, did not have significant CMCase activity, but these cells could bind purified 88-and 82-kDa CMCase but not 40.5-kDa CMCase. Because the 40.5-kDa CMCase is a fully active, truncated form of the CMCase, it appears that the N-terminal domain of the 88-kDa B 1 4 CMCase anchors the CMCase to the cells. Cells grown on cellobiose produced at least 10-fold more CMCase than the sucrose-grown cells, and the cellobiose-grown cells could only bind 15% as much CMCase as sucrose-grown cells. Virtually all of the CMCase activity of exponentially growing cultures was cell associated, but CMCase activity was eventually detected in the culture supernatant. On the basis of the observation that the 88-kDa CMCase was gradually converted to the 82-kDa CMCase when cultures reached the stationary phase without a change in specific activity, it appears that the 82-kDa protein is probably a proteolytic degradation product of the 88-kDa CMCase.

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Section: Discussionsupporting

confidence: 83%

“…P. ruminicola strains have as little as 20% DNA homology, and the B 1 4 strain is the most genetically distinct strain (11). The B 1 4 strain produces 88-and 82-kDa CMCases (13), while most other P. ruminicola strains produce smaller CMCases (1,16).…”

mentioning

confidence: 99%

The cellular location of Prevotella ruminicola beta-1,4-D-endoglucanase and its occurrence in other strains of ruminal bacteria

Gardner

Wells

Russell

et al. 1995

Appl Environ Microbiol

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“…A similarly high proportion of cultured isolates from the rumen have been reported to be Prevotella strains in some studies [14–16]. The genotypic and phenotypic variability within cultured strains of rumen Prevotella has been demonstrated [17,18] and recently the redefinition of the species P. ruminicola and the elevation of three different groups of strains to the species level was proposed [19]. Several major branches of the phylogenetic trees constructed from randomly cloned Prevotella/Bacteroides sequences lack any sequences derived from cultured strains [7,8].…”

Section: Introductionmentioning

confidence: 97%

Unravelling the genetic diversity of ruminal bacteria belonging to the CFB phylum

Ramšak¹,

Peterka²,

Tajima³

et al. 2000

FEMS Microbiology Ecology

106

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Molecular biology approaches were employed to examine the genetic diversity of bacteria from the Cytophaga/Flexibacter/Bacteroides (CFB) phylum in the rumen of cattle. By this means we were able to identify cultured strains that represent some of the larger CFB clusters previously identified only by PCR amplification and sequencing. Complete 16S rDNA sequences were obtained for 16 previously isolated rumen strains, including the type strains of Prevotella ruminicola, P. bryantii, P. brevis and P. albensis to represent a wide range of diversity. Phylogenetic analysis of cultured strains revealed the existence of three clusters of ruminal CFB: (i) a cluster of Prevotella strains, which have been found only in the rumen, including the two type strains, P. brevis GA33(T) and P. ruminicola 23(T); (ii) Prevotella spp. that cluster with prevotellas from other ecological niches such as the oral cavity and which include the type strains, P. bryantii B(1)4(T) and P. albensis M384(T); (iii) two Bacteroides spp. strains clustering with B. forsythus of oral origin. In order to establish whether the cultivated isolates cover the whole range of ruminal CFB genetic diversity, 16S rRNA gene sequences were amplified and cloned from DNA extracted from the same rumen samples (one cow in Slovenia, one in Scotland and three in Japan). Sequencing and phylogenetic analysis of 16S rRNA genes confirmed the existence of two superclusters of ruminal Prevotella, one exclusively ruminal and the other including non-ruminal species. In the case of ruminal Bacteroides spp., however, phylogenetic analysis revealed the existence of three new superclusters, one of which has as yet no cultivable counterpart. Interestingly, these Bacteroides clusters were represented almost exclusively by clone libraries from the Japanese cattle and only three sequences were from the European cattle. This study agrees with previous analyses in showing that rumen Prevotella/Bacteroides strains exhibit a remarkable degree of genetic diversity and suggests that different strain groupings may differ greatly in their recovery by cultural methods. The most important conclusion, however, is that cultured strains can be identified that represent some of the larger clusters previously identified only by PCR amplification and sequencing.

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“…bovis population, then it needs to be determined whether the populations that are likely to be encountered in the field are genetically homogenous and whether it is possible that a small number of phages can be found that will infect across the range likely to be encountered. Other common rumen bacteria, such as Prevotella ruminicola (Hudman and Gregg 1989;Mannarelli et al 1991) and Butyrivibrio fibrisolvens (Mannarelli 1988;Hudman and Gregg 1989;Forster et al 1996) have been found to be genetically heterogenous, comprising a variety of genetically distinct species and even genera. Streptococcus bovis strains appear much more closely related to each other, with ruminal strains likely to be representing at most two species (Nelms et al 1995).…”

Section: Introductionmentioning

confidence: 99%

Genetic homogeneity and phage susceptibility of ruminal strains of Streptococcus bovis isolated in Australia

Klieve

Heck

Prance

et al. 1999

Lett Appl Microbiol

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A .V . K L IE VE , G. L. H EC K, M .A . P R AN CE A ND Q. S HU . 1999. The genetic homogeneity of 37 strains of ruminal streptococci was investigated by comparing DNA fragment profiles on agarose gels following restriction endonuclease digestion with Hae III, Cfo I and Msp I. Thirty strains were indistinguishable from Streptococcus bovis strains, 2B, H24 and AR3. The remaining three strains were similar but not identical to a ruminal strain of Strep. intermedius (AR36). In addition, the susceptibility of these strains to infection by five bacteriophages was examined. Three of the phages (fSb02, fSb03 and fSb04) were specific to the strain of Strep. bovis from which they were isolated, while phages 2BV and fSb01 infected one and two strains, respectively, in addition to their primary host. It was concluded that although Strep. bovis is relatively homogeneous genetically, broad host range phages appear to be uncommon with this bacterial species.

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Taxonomic relationships among strains of the anaerobic bacterium Bacteroides ruminicola determined by DNA and extracellular polysaccharide analysis

Cited by 43 publications

References 39 publications

The cellular location of Prevotella ruminicola beta-1,4-D-endoglucanase and its occurrence in other strains of ruminal bacteria

The cellular location of Prevotella ruminicola beta-1,4-D-endoglucanase and its occurrence in other strains of ruminal bacteria

Unravelling the genetic diversity of ruminal bacteria belonging to the CFB phylum

Genetic homogeneity and phage susceptibility of ruminal strains of Streptococcus bovis isolated in Australia

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