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Aderkas, Patrick von; Rohr, René; Sundberg, Björn; Gutmann, M.; Dumont-BéBoux, Nicole; Lelu, Marie-Anne

doi:10.1023/a:1015245627220

Cited by 24 publications

(3 citation statements)

References 24 publications

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“…Tissue culture provides an alternate method for large-scale propagation of threatened and endangered plants, including orchid micropropagation using various explants. Somatic embryogenesis is one of the most promising approaches for plant propagation due to the production of large numbers of plantlets [10], the possibility of producing synthetic seeds [11], [12], the ability to store and rapidly mobilize germplasm for cryopreservation [13], the opportunity for genetic manipulation [14] and production of bioactive compounds within a short period of time [15], [16]. It is necessary to develop a method for mass clonal propagation and conservation to satisfy the pharmaceutical demand of this high value medicinal plant.…”

Section: Introductionmentioning

confidence: 99%

Direct somatic embryogenesis of Malaxis densiflora (A. Rich.) Kuntze

Mahendran

Bai

2016

Journal of Genetic Engineering and Biotechnology

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A protocol for induction of direct somatic embryogenesis and subsequent plant regeneration for the medicinally important and endangered plant of Malaxis densiflora has been developed for the first time. In the present study, in vitro seed derived protocorm explants were cultured on half strength Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), Picloram and Dicamba individually and in combination with cytokinins BAP, TDZ and Kn for its effectiveness to induce the differentiation of somatic embryos. The best response was observed in protocorms cultured half strength MS medium supplemented with 2,4-D at 3.39 μM and TDZ at 6.80 μM. Both epidermal and sub epidermal cells were involved in the formation of embryos. The proembryos developed into globular stage and subsequently developed into protocoms. Complete plantlets were formed after 60 days of culture. The plantlets were acclimatized in plastic pots containing sterilized vermiculite. The survival rate was 76%.

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Section: Introductionmentioning

confidence: 99%

Direct somatic embryogenesis of Malaxis densiflora (A. Rich.) Kuntze

Mahendran

Bai

2016

Journal of Genetic Engineering and Biotechnology

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“…Somatic embryogenesis by cell suspension culture offers an in vitro method for mass propagation of important medicinal plant species. In addition, somatic embryogenesis enables to produce large numbers of plantlets (Martin 2004) and bioactive compounds within a short span of time (Aderkas et al 2002;Jeong et al 2005). The technique is also suitable for germplasm conservation, the selection of genetic variants for desirable characters, the generation of somaclonal variants and for performing cellular genetic manipulations (Vasil 1988).…”

Section: Introductionmentioning

confidence: 99%

In vitro propagation of Psoralea corylifolia L. by somatic embryogenesis in cell suspension culture

Baskaran

Jayabalan

2009

Acta Physiol Plant

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An effective protocol was developed for in vitro propagation of Psoralea corylifolia via somatic embryogenesis in cell suspension culture. Embryogenic callus was obtained on Murashige and Skoog (MS) medium supplemented with 6 lM naphthaleneacetic acid (NAA) and 30 lM glutamine from transverse TCLs from 10-day-old hypocotyl explants with a 96.4% frequency. Embryogenic callus produced a higher number of somatic embryos (123.7 ± 1.24 per gram fresh weight callus) on MS medium containing 30 g l -1 sucrose, 1 lM NAA, 4 lM benzyladenine (BA), 15 lM glutamine and 2 lM abscisic acid (ABA) after 4 weeks of culture. Somatic embryos successfully germinated (97.6%) on MS medium containing 20 g l -1 sucrose, 8 g l -1 agar and supplemented with 2 lM BA, 1 lM ABA and 2 lM gibberellic acid (GA 3 ) within 2 weeks of culture. Somatic embryos developed into normal plants, which hardened with 100% efficiency in soil in a growth chamber. Plants were successfully transferred to greenhouse and subsequently established in the field. Plant survival percentage in the field differed with seasonal variations. Average psoralen content of 12.9 lg g -1 DW was measured in different stages of somatic embryo development by high-performance liquid chromatography (HPLC). This protocol will be helpful for efficient propagation of elite clones on a mass scale, conservation efforts of this species and for secondary metabolites production studies.

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“…Considering the timing from the start of P. radiata maturation to the end of ex vitro acclimatization (at least 25 weeks), it is very important to improve the last phases of the process from a technical and an economical point of view. In this sense, many years ago, different concentrations of abscisic acid (Von Aderkas et al 2002), activated charcoal (AC) addition (Yao and Wang 2020), modi cation of carbohydrates and PEG to modify the gel strength (Salaj et al 2019), the effect of ethylene (Neves et al 2021), the application of auxin transport inhibitors (Verma et al 2018) and redox compounds (Kudelko and Gaj 2019) and inhibitors of SE (Nic-Can et al 2015) were assayed to improve the yield of maturation process. However, the most in uential traits on embryo quality identi ed were the genotype (Kong and Von Aderkas, 2007), and the developmental stage of the tissue used as initial explant (Montalbán et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Multi-strategy approach towards optimization of maturation and germination in radiata pine somatic embryogenesis

Castander-Olarieta

Montalbán

Moncalean

2023

Plant Cell Tiss Organ Cult

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Somatic embryogenesis is a powerful technique for forestry improvement programs when implementing multi-varietal forestry. However, it still faces some bottlenecks to be e cient in many forestry species. In this work we have studied the effect of some physico-chemical modi cations at proliferation, maturation and germination stages of Pinus radiata D. Don somatic embryogenesis, as well as the impact of this modi cations on plant conversion, survival, and on the morphology and biochemical pro le (carbohydrate and amino acid analysis) of the resulting plantlets. Given the long culture period needed for maturation and germination in radiata pine, an increase of the plant yield could be very bene cial for its commercial application.A preculture of 14 days without plant growth regulators before maturation seems to be bene cial for the development and germination of somatic embryos. Before germination, a cold storage at 4ºC had no detrimental effect and even increased plant conversion in some embryogenic cell lines. During germination, supplementation of the culture medium with glutamine and a reduction of the sucrose content can improve the greenhouse acclimatization of the plants. Similarly, germinants were demonstrated to be in uenced by the light source: uorescent light enhanced root formation, whereas blue LED light increased the shoot height of somatic plants. Moreover, the highest plants showed lower contents of arginine, asparagine and total carbohydrates. Key MessageModi cation of the physico-chemical environment before or during somatic embryo maturation and germination improves the success of the process.

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Cited by 24 publications

References 24 publications

Direct somatic embryogenesis of Malaxis densiflora (A. Rich.) Kuntze

Direct somatic embryogenesis of Malaxis densiflora (A. Rich.) Kuntze

In vitro propagation of Psoralea corylifolia L. by somatic embryogenesis in cell suspension culture

Multi-strategy approach towards optimization of maturation and germination in radiata pine somatic embryogenesis

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