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Al-Safadi, B.; Ayyoubi, Z.; Jawdat, Dana

doi:10.1023/a:1006477224536

Cited by 47 publications

(9 citation statements)

References 20 publications

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“…It can create some novel and unique variations when the natural variability does not provide the genes for the desired trait (Velmurugan et al 2010). Related researches have been successfully carried out in potato (Al-Safadi et al 2000;Das et al 2000), banana (Bhagwat and Duncan 1998), cassava (Joseph et al 2004), sugar beet (Alikamanoglu 2002), lotus (Arunyanart and Soontronyatara 2002), wasabi (Hung and Johnson 2008), anthurium (Puchooa 2005), chrysanthemum (Barakat et al 2010), rose (Yamaguchi et al 2003), and narcissus (Lu et al 2007). …”

Section: Introductionmentioning

confidence: 99%

In vitro mutagenesis and identification of mutants via ISSR in lily (Lilium longiflorum)

et al. 2012

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An efficient in vitro mutagenesis protocol for Lilium longiflorum Thunb. cv. White fox has been established. The effect of 6-BA and NAA on adventitious bud formation from the bulblet-scale thin cell layers was tested. Results showed that the optimal medium for adventitious bud induction is MS basal medium supplemented with 2.0 mg/l 6-BA and 0.1 mg/l NAA. The differentiation frequency and the average number of adventitious buds reached 95.55% and 3.00, respectively. Various doses (0.0, 0.5, 1.0, 1.5, 2.0, and 2.5 Gy) of gamma rays were applied to investigate the effect of radiation on adventitious bud formation from bulblet-scale thin cell layers. The forming capacity of the adventitious buds significantly decreased with the increase of radiation dose. The results suggested that the optimal irradiation dose is 1.0 Gy. Dose of 1.0 Gy treatment resulted in 55.33% survival of irradiated bulblet-scale thin cell layers and 39.27% mutagenesis rate. The genetic variations among the morphological mutants were evaluated by DNA fingerprinting using ISSR molecular marker. The genetic variation frequency reached 36.06% using seven ISSR primers. Out of the 50 mutant lines transferred to the greenhouse, 9 were observed to have significantly different morphological characters than those of the controls.

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Section: Introductionmentioning

confidence: 99%

In vitro mutagenesis and identification of mutants via ISSR in lily (Lilium longiflorum)

et al. 2012

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“…In breeding by mutation, generally the frequency of mutations increases with dose and the rate at which the dose is applied, but the rate of regeneration in vitro is reduced [35]. In other crops, the use of low doses of gamma radiation has been reported to stimulate growth in vivo and in vitro [35] [40]. In P. vulgaris, low doses (0.5 Krad) stimulated growth (dry weight and fresh weight) and growth of in vitro calli [37].…”

Section: Discussionmentioning

confidence: 99%

“…This coincides with reports by Lu et al [35] that the critical level of irradiation with which mutations can be induced may be within the range of tolerance for regeneration in vitro. Breeding with ionizing radiation and/or chemical mutation in plant tissue culture has been reported in several crop plants, including the common bean [36] [37] [39], potato [40], pear [41] and peanut [42].…”

Section: Discussionmentioning

confidence: 99%

Effect of Gamma Irradiation and Selection with Fungus Filtrate (Rhizoctonia solani Kuhn) on the in Vitro Culture of Common Bean (Phaseolus vulgaris)

Solís-Ramos¹,

Valdez-Melara²,

Alvarado-Barrantes³

et al. 2015

AJPS

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The present investigation was undertaken to study the effect of gamma irradiation (dose from 10 to 100 Gy) and in vitro selection with fungus filtrate as selecting agent (concentration from 20% to 100%) on the susceptibility of the common bean to Rhizoctonia solani. The best results were found with a dose of 20 Gy or a concentration of 20% of fungus filtrate applied separately. These conditions were used to evaluate the combined effect of both approaches in a second experiment. The combined effect of irradiation and then selection adversely affected growth (height and roots) and survival of the in vitro plants. It may not be necessary to combine the variation generated by irradiation with the selection technique. For future assays we propose the application of: 1) gamma radiation, thereby inducing not only mutants with pathogen resistance, but also with other agronomic traits of interest. Later in the subculture MV4 potential fungus-resistant mutants will be evaluated in the field; or 2) selection pressure using fungus filtrate during three subcultures, which may be sufficient to induce the variation necessary to obtain in vitro plants resistant to fungus.

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“…Their propagation takes place primarily asexually, through tubers and microtubers (Al-safadi, Ayyoubi, & Jawdat, 2000;Li et al, 2005;Zhang, Li, Zhou, Takeuchi, & Yoneyama, 2006). However, there are two principal problems associated with the production of potato seed in a conventional manner: (1) the low rate of multiplication in the field and (2) the high susceptibility of potatoes to viral, bacterial and fungal diseases (Dobránszki, Magyar-Tábori, & Hudák, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Palavras-chaves: microtuberização; batata; Solanum tuberosum L.; reguladores de crescimento. 4 this process to the use of cytokinins such as benzylaminopurine (BAP) (Coleman & Coleman, 2000;Donnelly, Coleman, & Coleman, 2003;Lê, 1999;Sarkar, Pandey, & Sharma, 2006;Zhang et al, 2005b), and kinetin (K) (Aksenova et al, 2005;Aksenova, Konstantinova, Lozhnikova, Golyanovskaya, & Sergeeva, 2009;Coleman, Donnelly, & Coleman, 2001;Kefi, Pavlista, Meagher, & Read, 2000;Romanov et al, 2000), as well as the synergistic effect between them (Al-safadi et al, 2000). Although many plant hormones are assumed to be involved in the regulation of the tuberization, such as those mentioned previously, their effects finally depend on the gibberellin content of tissues (Xu, van Lammeren, Vermeer, & Vreugdenhil, 1998).…”

Section: Introductionmentioning

confidence: 99%

Effect of two cytokinins and a growth inhibitor on the in vitro tuberization of two genotypes of Solanum tuberosum L. cvs. Atlantic and Alpha

García-García

Azofeifa-Bolaños

Solano-Campos

et al. 2019

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This investigation was carried out to evaluate the effect of two cytokinins: 6-benzilaminopurine (BAP) (6.5 mg l-1) and kinetin (K) (2.5 mg l-1), as well as the growth inhibitor abscisic acid (ABA) (1.0 mg l-1) on the in vitro tuberization capacity of two potato varieties: Atlantic and Alpha. The basal culture medium MS (1962) was used as a control. The responses were different between varieties. In cv. Atlantic, the analysis of the number (NM), weight (WM) and diameter (DM) of microtubers indicated that the addition of growth regulators did not affect induction and development of microtubers. However, when BAP was used, a non-significant increment of 41 % was observed in the number of the microtubers compared to the control treatment, from 2.6 to 4.4. The addition of cytokinins and ABA to the medium did not have a significant impact on the development of microtubers. In cv. Alpha the cytokinins used without ABA increased the number of microtubers, which were larger and heavier than those of the control treatment. In this variety, ABA significantly reduced the values of the NM, WM and DM variables. The exogenous action of cytokinins in the culture medium is likely to have caused an endogenous hormonal imbalance in the Atlantic and Alpha genotypes which interfered with their innate microtuberization ability, a result that was even more evident for cv. Alpha, which showed the need to continue optimizing protocols of genotype-specific systems in potato tissue culture to increase yield and seed quality.

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Cited by 47 publications

References 20 publications

In vitro mutagenesis and identification of mutants via ISSR in lily (Lilium longiflorum)

In vitro mutagenesis and identification of mutants via ISSR in lily (Lilium longiflorum)

Effect of Gamma Irradiation and Selection with Fungus Filtrate (Rhizoctonia solani Kuhn) on the in Vitro Culture of Common Bean (Phaseolus vulgaris)

Effect of two cytokinins and a growth inhibitor on the in vitro tuberization of two genotypes of Solanum tuberosum L. cvs. Atlantic and Alpha

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