A comparison of monoclonal antibody productivity in different hollow fiber bioreactors

Lowrey, David M.; Murphy, Susan; Goffe, Randal A.

doi:10.1016/0168-1656(94)90020-5

Cited by 29 publications

(8 citation statements)

References 4 publications

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“…The interaction of cells with polymeric surfaces of biomaterials is an important subject of investigation for reasons such as optimal production of natural and recombinant bioproducts of cells [1], development of ideal nonthrombogenic bloodcontacting surfaces [2,3], and development of arti cial organs with hepatocyte [4], ¤ To whom correspondence should be addressed.…”

Section: Introductionmentioning

confidence: 99%

Growth of L929 cells on polymeric films prepared by Langmuir–Blodgett and casting methods

Higuchi

Tamiya

Tsubomura

et al. 2000

Journal of Biomaterials Science, Polymer Edition

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The growth and spreading of fibroblast, L929 cells, on various polymeric films prepared by the Langmuir-Blodgett (LB) and casting methods were investigated. L929 cells, which were cultivated on collagen and synthetic polymeric films prepared by the LB method, adhered and spread much more than those on synthetic films prepared by the casting method. This is explained by the fact that cell growth and cell spreading are suitable for L929 cells on the films having serum proteins that contain a high alpha-helix content, because LB films adsorbed those serum proteins estimated from the circular dichroism measurements of the films immersed in cell culture medium. An exponential relationship was observed from the plot of the cell density vs root mean square of roughness of the films, which is estimated by atomic force microscopy, whereas a linear relationship was observed from the plot of the spreading ratio vs the root mean square of roughness. It is suggested that the correlation between the cell growth or spreading ratio and surface roughness of the films where L929 cells were cultivated is considered to be more important than the correlation between the cell growth or spreading ratio and the contact angle of the films.

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Section: Introductionmentioning

confidence: 99%

Growth of L929 cells on polymeric films prepared by Langmuir–Blodgett and casting methods

Higuchi

Tamiya

Tsubomura

et al. 2000

Journal of Biomaterials Science, Polymer Edition

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“…Correlations between hybridoma cell productivity and culture material composition or properties have still not been established. The most frequently used membranes in hollow fiber systems are hydrophilic ones, such as cellulose–acetate or polymethylmethacrylate 10. Cell adhesion to these materials is minimal 11.…”

Section: Introductionmentioning

confidence: 99%

Supermacroporous polymer‐based cryogel bioreactor for monoclonal antibody production in continuous culture using hybridoma cells

Jain

Karande

Kumar

2010

Biotechnology Progress

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Cryogel matrices composed of different polymeric blends were synthesized, yielding a unique combination of hydrophilicity and hydrophobicity with the presence or absence of charged surface. Four such cryogel matrices composed of polyacrylamide-chitosan (PAAC), poly(N-isopropylacrylamide)-chitosan, polyacrylonitrile (PAN), and poly(N-isopropylacrylamide) were tested for growth of different hybridoma cell lines and production of antibody in static culture. All the matrices were capable for the adherence of hybridoma cell lines 6A4D7, B7B10, and H9E10 to the polymeric surfaces as well as for the efficient monoclonal antibody (mAb) production. PAAC proved to be relatively better in terms of both mAb production and cell growth. Further, PAAC cryogel was designed into three different formats, monolith, disks, and beads, and used as packing material for packed-bed bioreactor. Long-term cultivation of 6A4D7 cell line on PAAC cryogel scaffold in all the three formats could be successfully done for a period of 6 weeks under static conditions. Continuous packed-bed bioreactor was setup using 6A4D7 hybridoma cell line in the three reactor formats. The reactors ran continuously for a period of 60 days during which mAb production and metabolism of cells in the bioreactors were monitored periodically. The monolith bioreactor performed most efficiently over a period of 60 days and produced a total of 57.5 mg of antibody in the first 30 days (in 500 mL) with a highest concentration of 115 μg mL(-1) , which is fourfold higher than t-flask culture. The results demonstrate that appropriate chemistry and geometry of the bioreactor matrix for cell growth and immobilization can enhance the reactor productivity.

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“…When concerns about cruelty to mice for ascites production arose, the HFB proved to be a viable alternative [22,24,25]. Different types of HFBs have various effectivenesses for protein production [26,27]. Since the advent of recombinant‐protein production, the HFB has been used to grow a variety of mammalian cell lines for protein production, e.g.…”

Section: Introductionmentioning

confidence: 99%

Recombinant‐protein production in insect cells utilizing a hollow‐fibre bioreactor

Baxter

Panarello

Ajith

et al. 2006

Biotech and App Biochem

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Here, we demonstrate for the first time that the hollow-fibre bioreactor is an excellent tool for the production of Drosophila-expressed recombinant proteins. Using the example of the soluble extracellular portion of the human IL-5 (interleukin 5) receptor alpha expression in S2 (Schneider's Drosophila melanogaster cell line 2) cells, we found that it is possible to produce multi-milligram amounts of functional recombinant protein continuously for several months on a laboratory scale with minimal maintenance requirements. The insect cells grow to high density and express concentrated functional recombinant protein in a small volume, simplifying and economizing downstream purification.

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A comparison of monoclonal antibody productivity in different hollow fiber bioreactors

Cited by 29 publications

References 4 publications

Growth of L929 cells on polymeric films prepared by Langmuir–Blodgett and casting methods

Growth of L929 cells on polymeric films prepared by Langmuir–Blodgett and casting methods

Supermacroporous polymer‐based cryogel bioreactor for monoclonal antibody production in continuous culture using hybridoma cells

Recombinant‐protein production in insect cells utilizing a hollow‐fibre bioreactor

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