A competitive immunochromatographic strip assay for 17-α-hydroxy progesterone using colloidal gold nanoparticles

Tripathi, Vineeta; Nara, Seema; Singh, Kamya; Singh, Harpal; Shrivastav, Tulsidas G.

doi:10.1016/j.cca.2011.10.016

Cited by 20 publications

(11 citation statements)

References 13 publications

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“…This might occur as the Ab coating on GNPs may be varied because of the structural properties of Abs. The minimum Ab for coating those sizes of GNPs was generally reported as high concentrations, such as 40 or 150 µg/mL, in the literature (Shi et al, 2008;Mikawa et al, 2009;Tripathi et al, 2012;. Comparatively, this study made use of lower Ab concentrations for 36-nm GNPs.…”

Section: Synthesis Of Gold Nanoparticles and Conjugation With Antibodiesmentioning

confidence: 99%

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Optimizations needed for lateral flow assay for rapid detection of pathogenic E. coli

Çam

Öktem

2017

Turk J Biol

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IntroductionMany strains of Escherichia coli bacteria live in the gastrointestinal tracts of humans and animals. E. coli O157 was reported as a food pathogen after the hemorrhagic colitis outbreak of 1982 (Riley et al., 1983). O157:H7 is one of the most important E. coli strains transmitted from cattle/ animals to humans (Dorn and Angrick, 1991;Altekruse et al., 1997;Slutsker et al., 1998), either by contact, eating contaminated foods, drinking contaminated water, or passing from one person to another directly (Heiman et al., 2015). Infections of humans with E. coli O157:H7 can result in clinical issues like hemolytic uremic syndrome, acute nonbloody diarrhea, and thrombocytopenic thrombotic purpura. A comparison of the outbreaks of E. coli O157:H7 during 2003 and 2012 showed their abundance recently in the United States (Heiman et al., 2015). Hospitalizations and infections caused by E. coli O157 are still being seen, according to food safety news.Since pathogenic E. coli contaminates food and water easily (Liu and Li, 2002) and causes severe defects in humans and animals, its early detection is crucial for public health. Therefore, many kinds of detection platforms, like real-time PCR (

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Section: Synthesis Of Gold Nanoparticles and Conjugation With Antibodiesmentioning

confidence: 99%

“…Providing that the test procedure is correct, the control line is always visible. If no colored capture line or only a red color on the test line appears, the strip is invalid, and the test should be repeated using a new strip (Song et al, 2011;Tripathi et al, 2012). The test result is positive when both the test and control lines attain a red color.…”

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confidence: 99%

Optimizations needed for lateral flow assay for rapid detection of pathogenic E. coli

Çam

Öktem

2017

Turk J Biol

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“…A rapid lateral flow device for 17-OHP has been developed which could be used by patients to monitor their status. 136 Oral steroid treatment does not follow the natural pulsatile pattern of cortisol release. A modified release preparation of hydrocortisone, 137 and hydrocortisone delivered subcutaneously with an insulin pump 138 is under development to improve treatment of CAH.…”

Section: Monitoring Treatmentmentioning

confidence: 99%

17-Hydroxyprogesterone in children, adolescents and adults

Honour

2014

Ann Clin Biochem

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17-Hydroxyprogesterone (17-OHP) is an intermediate steroid in the adrenal biosynthetic pathway from cholesterol to cortisol and is the substrate for steroid 21-hydroxylase. An inherited deficiency of 21-hydroxylase leads to greatly increased serum concentrations of 17-OHP, while the absence of cortisol synthesis causes an increase in adrenocorticotrophic hormone. The classical congenital adrenal hyperplasia (CAH) presents usually with virilisation of a girl at birth. Affected boys and girls can have renal salt loss within a few days if aldosterone production is also compromised. Diagnosis can be delayed in boys. A non-classical form of congenital adrenal hyperplasia (NC-CAH) presents later in life usually with androgen excess. Moderately raised or normal 17-OHP concentrations can be seen basally but, if normal and clinical suspicion is high, an ACTH stimulation test will show 17-OHP concentrations (typically >30 nmol/L) above the normal response. NC-CAH is more likely to be detected clinically in females and may be asymptomatic particularly in males until families are investigated. The prevalence of NC-CAH in women with androgen excess can be up to 9% according to ethnic background and genotype. Mutations in the 21-hydroxylase genes in NC-CAH can be found that have less deleterious effects on enzyme activity. Other less-common defects in enzymes of cortisol synthesis can be associated with moderately elevated 17-OHP. Precocious puberty, acne, hirsutism and subfertility are the commonest features of hyperandrogenism. 17-OHP is a diagnostic marker for CAH but opinions differ on the role of 17OHP or androstenedione in monitoring treatment with renin in the salt losing form. This review considers the utility of 17-OHP measurements in children, adolescents and adults.

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“…After the addition of NaCl, we measured the optical density (OD) at 520 nm, 580 nm, and 600 nm. We used the ratio of the OD at 520 nm to that at 580 nm to assess stability and the ratio of the OD at 600 nm to that at 520 nm to assess polydispersity (20)(21)(22). We found the highest stability and lowest polydispersity when colloidal gold was conjugated to both anti-human IgG and IgA at pH 8.0 (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…To assess stability of the conjugate and to define the optimum pH and minimum concentration of antibody required for conjugating the colloidal gold, we used an aggregation assay (20)(21)(22). Specifically, we added 10% NaCl to the gold-protein suspension, incubated it for 10 min, and then assessed stability and polydispersity by measuring the absorbance at 520 nm, 580 nm, and 600 nm (20)(21)(22).…”

Section: Ethics Statementmentioning

confidence: 99%

Development of a Simple, Peripheral-Blood-Based Lateral-Flow Dipstick Assay for Accurate Detection of Patients with Enteric Fever

Khan¹,

Sayeed

Sultana³

et al. 2016

Clin Vaccine Immunol

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e Enteric fever is a systemic infection caused by typhoidal strains of Salmonella enterica and is a significant cause of mortality and morbidity in many parts of the world, especially in resource-limited areas. Unfortunately, currently available diagnostic tests for enteric fever lack sensitivity and/or specificity. No true clinically practical gold standard for diagnosing patients with enteric fever exists. Unfortunately, microbiologic culturing of blood is only 30 to 70% sensitive although 100% specific. Here, we report the development of a lateral-flow immunochromatographic dipstick assay based on the detection of Salmonella enterica serovar Typhi (S. Typhi) lipopolysaccharide (LPS)-specific IgG in lymphocyte culture secretion. We tested the assay using samples from 142 clinically suspected enteric fever patients, 28 healthy individuals residing in a zone where enteric fever is endemic, and 35 patients with other febrile illnesses. In our analysis, the dipstick detected all blood culture-confirmed S. Typhi cases (48/48) and 5 of 6 Salmonella enterica serovar Paratyphi A blood cultured-confirmed cases. The test was negative in all 35 individuals febrile with other illnesses and all 28 healthy controls from the zone of endemicity. The test was positive in 19 of 88 individuals with suspected enteric fever but with negative blood cultures. Thus, the dipstick had a sensitivity of 98% compared to blood culture results and a specificity that ranged from 78 to 100% (95% confidence interval [CI], 70 to 100%), depending on the definition of a true negative. These results suggest that this dipstick assay can be very useful for the detection of enteric fever patients especially in regions of endemicity.

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A competitive immunochromatographic strip assay for 17-α-hydroxy progesterone using colloidal gold nanoparticles

Cited by 20 publications

References 13 publications

Optimizations needed for lateral flow assay for rapid detection of pathogenic E. coli

Optimizations needed for lateral flow assay for rapid detection of pathogenic E. coli

17-Hydroxyprogesterone in children, adolescents and adults

Development of a Simple, Peripheral-Blood-Based Lateral-Flow Dipstick Assay for Accurate Detection of Patients with Enteric Fever

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