A Decade of Advances in Iridovirus Research

Williams, Trevor; Barbosa-Solomieu, Valérie; Chinchar, V. Gregory

doi:10.1016/s0065-3527(05)65006-3

Cited by 311 publications

(295 citation statements)

References 308 publications

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“…L genes are expressed after the onset of viral DNA replication, and can be blocked by DNA replication and protein synthesis inhibitor [7,8]. In this study, RGV 88R transcripts and expression were blocked by both DNA replication inhibitor and protein synthesis inhibitor (Fig.…”

Section: Discussionmentioning

confidence: 51%

“…As described above, iridoviruses displays a complex gene regulation strategy in which genes are expressed in three main temporal kinetics stages: IE genes, E genes and L genes [5][6][7][8]. L genes are expressed after the onset of viral DNA replication, and can be blocked by DNA replication and protein synthesis inhibitor [7,8].…”

Section: Discussionmentioning

confidence: 99%

“…RGV is a large DNA virus and is closely related to frog virus 3 (FV3), the type species of genus Ranavirus (family Iridoviridae) [2][3][4]. Iridoviruses display a complex gene regulation strategy in which genes are expressed in three main temporal stages: immediate early (IE) genes, early (E) genes and late (L) genes, which can be defined experimentally by using DNA replication and protein synthesis inhibitors [5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

“…To date, the genomes of twelve iridoviruses, including FV3, have been completely sequenced [8,9], and 26 core genes have been predicted to exist in all iridoviruses [10]. One of these is a gene belonging to ERV1 (essential for respiration and viability) family.…”

Section: Introductionmentioning

confidence: 99%

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Cloning, expression and subcellular distribution of a Rana grylio virus late gene encoding ERV1 homologue

2008

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An essential for respiration and viability (ERV1) homologue, 88R, was cloned and characterized from Rana grylio virus (RGV). Database searches found its homologues in all sequenced iridoviruses, and sequence alignment revealed a highly conserved motif shared by all ERV1 family proteins: Cys-X-X-Cys. RT-PCR and western blot analysis revealed that 88R begins to transcribe and translate at 6 h postinfection (p.i.) and remains detectable at 48 h p.i. during RGV infection course. Furthermore, using drug inhibition analysis by a de novo protein synthesis inhibitor and a viral DNA replication inhibitor, RGV 88R was classified as a late (L) viral gene during the in vitro infection. 88R-EGFP fusion protein was observed in both the cytoplasm and nucleus of pEGFP-N3-88R transfected EPC cells. Although result of immunofluorescence is similar, 88R protein was not detected in viromatrix. Moreover, function of RGV 88R on virus replication were evaluated by RNAi assay. Nevertheless, effect of knockdown of RGV 88R expression on virus replication was not detected in cultured fish cell lines. Collectively, current data indicate that RGV 88R was a late gene of iridovirus encoding protein that distributed both the cytoplasm and nucleus.

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Section: Discussionmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cloning, expression and subcellular distribution of a Rana grylio virus late gene encoding ERV1 homologue

2008

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“…Iridoviruses are large DNA viruses that replicate in the cytoplasm of infected cells [1]. The viral genome is both circularly permutated and terminally redundant and is a double-stranded DNA genome ranging from 103 to 212 kbp in length [2].…”

Section: Introductionmentioning

confidence: 99%

Rana grylio virus thymidine kinase gene: an early gene of iridovirus encoding for a cytoplasmic protein

et al. 2009

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The presence of thymidine kinase (TK) is a feature of many large DNA viruses. Here, a TK gene homologue was cloned and characterized from Rana grylio virus (RGV), a member of family Iridoviridae. RGV TK encodes a protein of 195 aa with a predicted molecular mass of 22.1 kDa. Homologues of the protein were present in all the currently sequenced iridoviruses, and phylogenetic analysis showed that it was much close to cellular TK type 2 (TK2), deoxycytidine kinase (dCK) and deoxyguanosine kinase (dGK). Subsequently, Western blotting revealed TK expression increased with time from 6 h postinfection in RGV-infected cells. Using drug inhibition analysis by protein synthesis inhibitor (cycloheximide) and DNA replication inhibitor (cytosine arabinofuranoside), RGV TK was classified as the early expression gene during in vitro infection. Subcellular localization by TK-GFP fusion protein expression and immunofluorescence staining showed RGV TK was an exclusively cytoplasmic protein in fish cells. Collectively, current data indicate that RGV TK was an early gene of iridovirus which encoded a cytoplasmic protein in fish cells.

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Structural Biology and Functional Genomics of the Shrimp White Spot Syndrome Virus and Singapore Grouper Iridovirus

Hew

2011

Aquaculture Biotechnology

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A Decade of Advances in Iridovirus Research

Cited by 311 publications

References 308 publications

Cloning, expression and subcellular distribution of a Rana grylio virus late gene encoding ERV1 homologue

Cloning, expression and subcellular distribution of a Rana grylio virus late gene encoding ERV1 homologue

Rana grylio virus thymidine kinase gene: an early gene of iridovirus encoding for a cytoplasmic protein

Structural Biology and Functional Genomics of the Shrimp White Spot Syndrome Virus and Singapore Grouper Iridovirus

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