A direct RP-HPLC method for the determination of furanic aldehydes and acids in honey

Spano, Nadia; Ciulu, Marco; Floris, Ignazio; Panzanelli, Angelo; Pilo, Maria Itria; Piu, P.; Salis, Severyn; Sanna, Gavino

doi:10.1016/j.talanta.2008.11.015

Cited by 55 publications

(45 citation statements)

References 25 publications

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“…Following these introductory considerations and pursuing our interest in the qualitative and quantitative determination of minority compounds in beehive products (Ciulu et al, 2011(Ciulu et al, , 2013Sanna et al, 2000;Spano et al, 2006Spano et al, , 2008Spano et al, , 2009aSpano et al, , 2009b, we assessed and validated a potentiometric FISE method to directly measure the free fluoride concentration in honey. The procedure was also successfully applied to a number of selected unifloral honeys produced in different parts of the island of Sardinia, Italy.…”

Section: Introductionmentioning

confidence: 99%

Free fluoride determination in honey by ion-specific electrode potentiometry: Method assessment, validation and application to real unifloral samples

Spano

Guccini

Ciulu

et al. 2018

Arabian Journal of Chemistry

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Surprisingly, a reliable method for measuring the concentration of free fluoride ions in honey is still missing from the literature, notwithstanding the generally recognized importance of the analyte and the matrix. To fill this gap, this study proposes and validates a straightforward ion-specific electrode potentiometric method for this task. The method offers very low detection and quantification limits (6.7 lg kg À1 and 25 lg kg À1 , respectively), good linearity (R 2 > 0.994), good sensitivity (typically 55 ± 3 mV for an order of magnitude of concentration) in an unusually low concentration interval (between 0.020 and 1 mg L À1 ), and acceptable precision and bias. The method was applied to 30 unifloral (thistle, eucalyptus and strawberry tree) honey samples from Sardinia, Italy. The amount of free fluoride ions found in these honeys appears to be lower than Abbreviations: FISE, fluoride ion-selective electrode; TISAB, total ionic strength adjusting buffer; EDTA, ethylenediaminetetraacetic acid disodium salt; CDTA, 2-[2-[bis(2-hydroxy-2-oxoethyl)amino]cyclohexyl]-(2-hydroxy-2-oxoethyl)aminoethanoic acid; EC, external calibration method; MSA, multiple standard additions method; CRM, certified reference material; LOD, limit of detection; LOQ, limit of quantification * Corresponding author.

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Section: Introductionmentioning

confidence: 99%

Free fluoride determination in honey by ion-specific electrode potentiometry: Method assessment, validation and application to real unifloral samples

Spano

Guccini

Ciulu

et al. 2018

Arabian Journal of Chemistry

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“…For example, furanic aldehydes were reported to be present in honey 6 and popped popcorn. 3-Furaldehyde (3FA) is among the furan pollutants found in atmosphere, and the investigation of its structure and chemical reactivity may contribute to assess the atmospheric reactivity of such compounds and to elucidate their final fates in the atmosphere.…”

Section: Introductionmentioning

confidence: 99%

Photoisomerization and Photochemistry of Matrix-Isolated 3-Furaldehyde

2010

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3-Furaldehyde (3FA) was isolated in an argon matrix at 12 K and studied using FTIR spectroscopy and quantum chemistry. The molecule has two conformers, with trans and cis orientation of the OdCsCdC dihedral angle. At the B3LYP/6-311++G(d,p) level of theory, the trans form was computed to be ca. 4 kJ mol -1 more stable than the cis form. The relative stability of the two conformers was explained using the natural bond orbital (NBO) method. In fair agreement with their calculated relative energies and the high barrier of rotamerization (ca. 34 kJ mol -1 from trans to cis), the trans and cis conformers were trapped in an argon matrix from the compound room temperature gas phase in proportion ∼7:1. The experimentally observed vibrational signatures of the two forms are in a good agreement with the theoretically calculated spectra. Broad-band UV-irradiation (λ > 234 nm) of the matrix-isolated compound resulted in partial trans f cis isomerization, which ended at a photostationary state with the trans/cis ratio being ca. 1.85:1. This result was interpreted based on results of time-dependent DFT calculations. Irradiation at higher energies (λ > 200 nm) led to decarbonylation of the compound, yielding furan, cyclopropene-3-carbaldehyde, and two C 3 H 4 isomers: cyclopropene and propadiene.

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“…Em particular, alguns estudos mostram que o HMF está entre as substâncias de risco de citotoxicidade, genotoxicidade e atividade mutagênica. 9 Existem vários métodos propostos para determinar o HMF em mel, espectrofotometricamente pelo método de Winkler ou modificações desse método e o método de White que envolvem medidas de absorvância na região ultravioleta (UV) e por cromatografia líquida de alta eficiência (CLAE). Este último parece ser o mais apropriado devido a interferências provocadas por substâncias derivadas, provavelmente, do aquecimento ou estocagem inadequada quando utiliza os métodos UV.…”

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Validação de método para a determinação de 5-hidroximetilfurfural em mel por cromatografia líquida e sua influência na qualidade do produto

Lemos

Santos

2010

Quím. Nova

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Recebido em 16/11/09; aceito em 9/4/10; publicado na web em 24/8/10 METHOD VALIDATION TO HMF DETERMINATION IN HONEY BY HPLC-UV AND ITS INFLUENCE ON THE PRODUCT QUALITY. In honey 5-hydroxymethyl-2-furaldehyde (HMF) is one of the most typical products of degradation: it is usually absent in fresh honey, but its concentration tends to rise as a result of heating processes or long-term storage. The validation protocol was performed in terms of detection and quantification limits, precision (by repeatability and intermediate precision), linearity and accuracy (by recovery tests). The method has been tested on 15 honey samples of different ages and geographical origin. HMF correlated highly with the age of the samples has been considered a very important parameter to put these honeys on the market or not and/or to estimate their shelf life.Keywords: HMF; honey; HPLC-UV. INTRODUÇÃOO mel é um alimento natural produzido por abelhas melíferas, a partir do néctar ou secreções de plantas, modificado e armazenado em colmeias.1 É um produto semilíquido que contém uma mistura complexa de carboidratos (principalmente glicose e frutose), ácidos orgânicos, lactonas, aminoácidos, minerais, vitaminas, enzimas, pólen, cera e pigmentos. 2O crescente consumo do mel nas últimas décadas, estimulado pelas propriedades reconhecidamente benéficas à saúde humana, vem exigindo um controle de qualidade cada vez mais eficiente deste produto. Os mais importantes parâmetros para avaliar a qualidade do mel são teores de contaminantes (antibióticos, pesticidas, metais pesados), origem floral, área de produção e tempo de prateleira. O parâmetro mais utilizado para conferir se ocorreu um aquecimento indesejável, ou avaliar o tempo de estocagem do mel, são os teores de 5-hidroximetilfurfural (HMF) e o índice de diastase. 3O HMF geralmente não está presente em méis frescos e seu conteúdo tende a aumentar durante o processo de aquecimento e o longo tempo de estocagem, 4-6 pela reação de Maillard em carboidratos ou desidratação catalítica ácida das hexoses. O HMF é um dos principais produtos de degradação no mel sendo o aumento de sua concentração influenciada pelo baixo pH, acidez total, minerais, origem botânica, umidade, temperatura e stress fotoquímico. , com exceção para os méis oriundos de países de temperaturas tropicais e para os méis com baixos níveis enzimáticos, cujos níveis máximos de HMF estabelecidos são de 80 e 15 mg kg -1 , respectivamente. 7,8 A flutuação de HMF em méis uniflorais armazenados à temperatura ambiente tem sido considerado como um parâmetro muito importante para certificar a qualidade dos méis disponíveis no mercado ou estimar sua estabilidade de prateleira. 3A presença de componentes potencialmente tóxicos (tais como aldeídos reativos) em alimentos vem aumentando a atenção de agências protetoras do consumidor e de controle de qualidade. Em particular, alguns estudos mostram que o HMF está entre as substâncias de risco de citotoxicidade, genotoxicidade e atividade mutagênica.9 Existem vários métodos propostos para determina...

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A direct RP-HPLC method for the determination of furanic aldehydes and acids in honey

Cited by 55 publications

References 25 publications

Free fluoride determination in honey by ion-specific electrode potentiometry: Method assessment, validation and application to real unifloral samples

Free fluoride determination in honey by ion-specific electrode potentiometry: Method assessment, validation and application to real unifloral samples

Photoisomerization and Photochemistry of Matrix-Isolated 3-Furaldehyde

Validação de método para a determinação de 5-hidroximetilfurfural em mel por cromatografia líquida e sua influência na qualidade do produto

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