A functional centromere lacking CentO sequences in a newly formed ring chromosome in rice

Yang, Rui; Li, Yafei; Su, Yan; Shen, Yi; Tang, Ding; Luo, Qiong; Cheng, Zhihui

doi:10.1016/j.jgg.2016.09.006

Cited by 11 publications

(6 citation statements)

References 48 publications

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“…CenH3 is essential for chromosome segregation in all organisms tested, and the removal of CenH3 is accompanied by the inability to assemble other kinetochore components (Choo, 2001). In rice, CenH3 was colocalized with the CRR and CentO repeats, which were enriched in the CenH3-bound fraction in a chromatin immunoprecipitation assay (Yang et al, 2016). Our data showing the large increase of 24-nucleotide siRNAs and CHH hypermethylation of CRR1 elements in the osddm1a/1b mutant confirm that RdDM of centromere repeats is negatively regulated by OsDDM1.…”

Section: Osddm1 Function In Rddmsupporting

confidence: 68%

DDM1 Represses Noncoding RNA Expression and RNA-Directed DNA Methylation in Heterochromatin

Tan

Jiang

et al. 2018

Plant Physiol.

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C methylation of DNA, which occurs at CG, CHG, and CHH (H = A, C, or T) sequences in plants, is a hallmark for the epigenetic repression of repetitive sequences. The chromatin-remodeling factor DECREASE IN DNA METHYLATION1 (DDM1) is essential for DNA methylation, especially at CG and CHG sequences. However, its potential roles in RNA-directed DNA methylation (RdDM) and in chromatin function are not completely understood in rice (). In this work, we used high-throughput approaches to study the function of rice DDM1 (OsDDM1) in RdDM and the expression of noncoding RNA. We show that loss of function of OsDDM1 results in ectopic CHH methylation of transposable elements and repeats. The ectopic CHH methylation was dependent on rice DOMAINS REARRANGED METHYLTRANSFERASE2, a DNA methyltransferase involved in RdDM. Mutations in lead to decreases of histone H3K9me2 and increases in the levels of heterochromatic small RNA and long noncoding RNA. In particular, OsDDM1 was found to be essential to repress the transcription of the two repetitive sequences, Centromeric Retrotransposons of Rice1 and the dominant centromeric CentO repeats. These results suggest that OsDDM1 antagonizes RdDM at heterochromatin and represses the tissue-specific expression of noncoding RNA from repetitive sequences in the rice genome.

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Section: Osddm1 Function In Rddmsupporting

confidence: 68%

DDM1 Represses Noncoding RNA Expression and RNA-Directed DNA Methylation in Heterochromatin

Tan

Jiang

et al. 2018

Plant Physiol.

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“…The primary antibodies used in immunofluorescence were anti-OsREC8, anti-ZEP1, anti-OsCOM1, anti-OsDMC1 and anti-γH2AX (Wang et al, 2010, 2016; Shao et al, 2011; Ji et al, 2012). FISH analysis was conducted according to Yang et al (2016). Original images were captured under Zeiss A2 fluorescence microscope with a micro CCD camera.…”

Section: Methodsmentioning

confidence: 99%

OsRAD17 Is Required for Meiotic Double-Strand Break Repair and Plays a Redundant Role With OsZIP4 in Synaptonemal Complex Assembly

Zhang

Xue

et al. 2018

Front. Plant Sci.

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The repair of SPO11-dependent double-strand breaks (DSBs) by homologous recombination (HR) ensures the correct segregation of homologous chromosomes. In yeast and human, RAD17 is involved in DNA damage checkpoint control and DSB repair. However, little is known about its function in plants. In this study, we characterized the RAD17 homolog in rice. In Osrad17 pollen mother cells (PMCs), associations between non-homologous chromosomes and chromosome fragmentation were constantly observed. These aberrant chromosome associations were dependent on the formation of programmed DSBs. OsRAD17 interacts with OsRAD1 and the meiotic phenotype of Osrad1 Osrad17 is indistinguishable from the two single mutants which have similar phenotypes, manifesting they could act in the same pathway. OsZIP4, OsMSH5 and OsMER3 are members of ZMM proteins in rice that are required for crossover formation. We found that homologous pairing and synapsis, which was roughly unaffected in Oszip4 and Osrad17 single mutant, was severely disturbed in the Oszip4 Osrad17 double mutant. Similar phenotypes were observed in the Osmsh5 Osrad17 and Osmer3 Osrad1 double mutants, suggesting the cooperation between the checkpoint proteins and ZMM proteins in assuring accurate HR in rice.

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“…For DAPI staining and FISH assays, young spikelets at male meiotic stages were collected and fixed with Carnoy's solution (ethanol:glacial acetic 3:1 [v/v]), and then prepared as described previously (Cheng, 2013). The sequences of the telomere and centromere FISH probes were determined according to previous studies (Mizuno et al, 2006;Yang et al, 2016). The sequence of the 5S rDNA FISH probe labeled with fluorescein isothiocyanate is listed in Supplemental Table S1.…”

Section: Microscopy Analysismentioning

confidence: 99%

A Multiprotein Complex Regulates Interference-Sensitive Crossover Formation in Rice

et al. 2019

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A functional centromere lacking CentO sequences in a newly formed ring chromosome in rice

Cited by 11 publications

References 48 publications

DDM1 Represses Noncoding RNA Expression and RNA-Directed DNA Methylation in Heterochromatin

DDM1 Represses Noncoding RNA Expression and RNA-Directed DNA Methylation in Heterochromatin

OsRAD17 Is Required for Meiotic Double-Strand Break Repair and Plays a Redundant Role With OsZIP4 in Synaptonemal Complex Assembly

A Multiprotein Complex Regulates Interference-Sensitive Crossover Formation in Rice

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