A generic microfluidic biosensor of G protein-coupled receptor activation – impedance measurements of reversible morphological changes of reverse transfected HEK293 cells on microelectrodes

Srivastava, Saurabh; Ramaneti, R.; Roelse, Margriet; Tong, Hien D.; Vrouwe, E.X.; Brinkman, Aldo G. M.; Smet, Louis C. P. M. de; Rijn, C.J.M. van; Jongsma, M.A.

doi:10.1039/c5ra04976h

Cited by 6 publications

(5 citation statements)

References 45 publications

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“…These sensors are now widely applied in detections of various scales, e.g. DNA [21][22][23] , proteins 24,25 , cells 26,27 and bacteria 28 . For impedance-based measurements, an electrode-electrolyte double layer will be formed at the electrode surface, and this double layer capacitance behaves as a parasitic component to reduce the sensitivity 29 .…”

Section: Introductionmentioning

confidence: 99%

Inkjet-printed Ag electrodes on paper for high sensitivity impedance measurements

Jiang

et al. 2016

RSC Adv.

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Section: Introductionmentioning

confidence: 99%

Inkjet-printed Ag electrodes on paper for high sensitivity impedance measurements

Jiang

et al. 2016

RSC Adv.

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“…In this study, electrochemical sensors were used because of high precision and integration ability [2,[9][10][11][12]. The sensor consists of working, counter, and reference electrodes.…”

Section: Introductionmentioning

confidence: 99%

“…Given that these electrodes are integrated in the microchamber, the replacement of the conventional reference electrode by a pseudo-reference electrode (PRE) facilitates the miniaturization of the analytical system. The microfluidic biosensor integrated PRE has been used in many studies for the determination of medicament [13], cell morphological changes [12], heavy metals [14], and glucose [2] in aqueous solution. However, little research has been dedicated to the synthesis of nanostructures inside the integrated three-electrode chip.…”

Section: Introductionmentioning

confidence: 99%

In‐Channel‐Grown Polypyrrole Nanowire for the Detection of DNA Hybridization in an Electrochemical Microfluidic Biosensor

Tran

Chu

Quan

et al. 2015

Journal of Nanomaterials

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A triple electrode setup with a Pt pseudo-reference electrode integrated in a polydimethylsiloxane- (PDMS-) based microchamber was designed and fabricated. The integrated electrodes were deposited onto SiO2/Si substrate by sputtering. The PDMS microchamber was patterned using an SU-8 mold and sealed with electrodes in oxygen plasma. Polypyrrole nanowires (PPy NWs) were electrochemically grown in situ at an accurate position of the working electrode in the sealed microchamber instead of in an open system. The DNA probe sequences were simply introduced into the channel to form bonds with the nanowires. A detection limit of 20 pM was achieved using a lock-in amplifier. The electrochemical characteristics produced by the hybridization of DNA strands in the microchamber showed a good signal/noise ratio and high sensitivity. Measurement of the DNA sensor in narrow space also required much less volume of the analytical sample compared with that in an open measuring cell. Results showed that this simple system can potentially fabricate nanostructures and detect bio/chemical molecules in a sealed system.

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“…gold, 6,20,71-73 silver [74][75][76] and platinum, [77][78] or other inert metals, such as stainless steel [79][80] and chromium, 81 are normally used. On the other hand, parallel impedances are dominated by the smallest magnitudes, requiring highly insulating substrates, for example glass, [71][72]82 silicon, 73,[83][84][85] , quartz 86 and alumina 82,87 . The recent attempts to reduce costs for disposable sensing units escalated research into flexible materials, including plastics 88 and bio-based materials such as cellulose-related substrates.…”

Section: Substrates and Electrodesmentioning

confidence: 99%

“…This represents a problem since some biosensing applications require lower amplitudes (down to 10 mV). 16,85,152,188 Another main limitation is the low maximum current limit, which constrains the impedance range to values over 1 kΩ. 33 We included an external OP-AMP AD8606 in "follower" mode shown in Figure 16 which increased the current limit allowing for impedances down to 100 Ω.…”

Section: Output Amplification (B)mentioning

confidence: 99%

Development of a Portable Impedance Spectrometer

Buscaglia¹

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 Mamá, for being unconditionally present, for helping me with the software development for so many days, and most important, for being my mother. Sisters, grandparents (Sílvia and Vivaldo, you too!), uncles and aunts, cousins, Mariana and Marcelo, for accompanying me during these years. Friends from São Carlos, Bariloche and abroad, all essential for me to work every day. Bianca, for the deep friendship, for the teachings, and especially for the months of quarantine together, which were so important for this project to succeed. Larissa, for being my partner and having participated with much love in my daily life during this adventure. Professor João P. Carmo, for your guidance with the electronics and for your collaboration during the writing of the review article and patent application.  Tía Mariana, for your thoughtful collaboration on designing the logo and the casing of Simple-Z.  Gounella, Poste, Tiago and Melk, for all your time helping me in the fabrication of Simple-Z.  Paulo, for your time and dedication on helping me with the sodium sulfate, among others, experiments.  Tío Car, for your very careful mentoring during the experiments with cyanobacteria. Fellipe, for the many hours spent in the laboratory helping me on the experiments with cyanobacteria and for helping me write the correspondent part in this dissertation. Andrey and Juliana, for working with me over this years and especially on the article on SARS-CoV-2. Professor Margaret, even though the pandemic brought down our plans, for your willingness and efforts on receiving me in Ireland for working together. Willyan, Priscila and all Biosens, for supporting my personal development. All the co-authors of the works published throughout my Masters, it was a pleasure working with you.

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A generic microfluidic biosensor of G protein-coupled receptor activation – impedance measurements of reversible morphological changes of reverse transfected HEK293 cells on microelectrodes

Abstract: Flowcell with micro-IDEs (250–500 μm) covered with both stable and reverse transfected cells overexpressing membrane receptors to demonstrate impedance responses to serial injections of analyte.

Cited by 6 publications

References 45 publications

Inkjet-printed Ag electrodes on paper for high sensitivity impedance measurements

Inkjet-printed Ag electrodes on paper for high sensitivity impedance measurements

In‐Channel‐Grown Polypyrrole Nanowire for the Detection of DNA Hybridization in an Electrochemical Microfluidic Biosensor

Development of a Portable Impedance Spectrometer

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