A glycoconjugate-based gold nanoparticle approach for the targeted treatment of <i>Pseudomonas aeruginosa</i> biofilms

Zhang, Chao; Shi, De Tai; Yan, Kai-Cheng; Sedgwick, Adam; Chen, Guo Rong; He, Xiao; James, Tony D.; Yan, Bing; Hu, Xi; Chen, Daijie

doi:10.1039/d0nr05365a

Cited by 26 publications

(20 citation statements)

References 55 publications

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“…This research offers insights into the construction of imaging probes that can target pathogen-conserved metabolic pathways, making possible the development of simple and clinically transformable fluorescent agents and materials for the timely monitoring of bacterial infections in situ . The development of fluorescent probes that can be activated by species-specific biomarkers in order to further enhance imaging precision is underway in our group. − Moreover, owing to the inherent antimicrobial properties of GO, we are also exploring the use of PABA-DCM@GO hydrogel dressing for image-guided photodynamic and/or photothermal therapy of bacterial infections. − …”

Section: Discussionmentioning

confidence: 99%

Metabolically Specific In Situ Fluorescent Visualization of Bacterial Infection on Wound Tissues

Zhong

Yang

et al. 2022

ACS Appl. Mater. Interfaces

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The ability to effectively detect bacterial infection in human tissues is important for the timely treatment of the infection. However, traditional techniques fail to visualize bacterial species adhered to host cells in situ in a target-specific manner. Dihydropteroate synthase (DHPS) exclusively exists in bacterial species and metabolically converts p-aminobenzoic acid (PABA) to folic acid (FA). By targeting this bacterium-specific metabolism, we have developed a fluorescent imaging probe, PABA-DCM, based on the conjugation of PABA with a long-wavelength fluorophore, dicyanomethylene 4H-pyran (DCM). We confirmed that the probe can be used in the synthetic pathway of a broad spectrum of Gram-positive and negative bacteria, resulting in a significantly extended retention time in bacterial over mammalian cells. We validated that DHPS catalytically introduces a dihydropteridine group to the amino end of the PABA motif of PABA-DCM, and the resulting adduct leads to an increase in the FA levels of bacteria. We also constructed a hydrogel dressing containing PABA-DCM and graphene oxide (GO), termed PABA-DCM@GO, that achieves target-specific fluorescence visualization of bacterial infection on the wounded tissues of mice. Our research paves the way for the development of fluorescent imaging agents that target species-conserved metabolic pathways of microorganisms for the in situ monitoring of infections in human tissues.

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Section: Discussionmentioning

confidence: 99%

Metabolically Specific In Situ Fluorescent Visualization of Bacterial Infection on Wound Tissues

Zhong

Yang

et al. 2022

ACS Appl. Mater. Interfaces

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“…Given the associated cell surface lectins (LecA, B), galactose- or fucose-functionalised AuNPs have been used to target, detect and kill Pseudomonas aeruginosa ( Zhang et al, 2020 ). The functionalisation was performed via copper-free click chemistry between azidobutyl glycosides and a cyclooctyne-based thioctic acid linker ( Karamanska et al, 2005 ).…”

Section: Bacteriamentioning

confidence: 99%

Recent Developments in the Use of Glyconanoparticles and Related Quantum Dots for the Detection of Lectins, Viruses, Bacteria and Cancer Cells

et al. 2021

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Carbohydrate-coated nanoparticles—glyconanoparticles—are finding increased interest as tools in biomedicine. This compilation, mainly covering the past five years, comprises the use of gold, silver and ferrite (magnetic) nanoparticles, silicon-based and cadmium-based quantum dots. Applications in the detection of lectins/protein toxins, viruses and bacteria are covered, as well as advances in detection of cancer cells. The role of the carbohydrate moieties in stabilising nanoparticles and providing selectivity in bioassays is discussed, the issue of cytotoxicity encountered in some systems, especially semiconductor quantum dots, is also considered. Efforts to overcome the latter problem by using other types of nanoparticles, based on gold or silicon, are also presented.

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“…Human ASGPR is mainly expressed in hepatocytes, and there are approximately 500,000–1,000,000 receptors per person. The reported ASGPR-specific ligands include galactose [ 31 ], lactose [ 32 ], acetylgalactosamine [ 33 ], and asialofetuin [ 34 ]. Ligand modification could promote the delivery of active targeted nanocarriers to the hepatocytes.…”

Section: Characteristics Of Nanoparticle Drug Delivery Systems For Anti-hbv Therapymentioning

confidence: 99%

Advances in Nanoparticle Drug Delivery Systems for Anti-Hepatitis B Virus Therapy: A Narrative Review

Miao

Gao

et al. 2021

IJMS

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Chronic hepatitis B (CHB) is an infectious viral disease that is prevalent worldwide. Traditional nucleoside analogues, as well as the novel drug targets against hepatitis B virus (HBV), are associated with certain critical factors that influence the curative effect, such as biological stability and safety, effective drug delivery, and controlled release. Nanoparticle drug delivery systems have significant advantages and have provided a basis for the development of anti-HBV strategies. In this review, we aim to review the advances in nanoparticle drug delivery systems for anti-hepatitis B virus therapy by summarizing the relevant literature. First, we focus on the characteristics of nanoparticle drug delivery systems for anti-HBV therapy. Second, we discuss the nanoparticle delivery systems for anti-HBV nucleoside drugs, gene-based drugs, and vaccines. Lastly, we provide an overview of the prospects for nanoparticle-based anti-HBV agents.

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A glycoconjugate-based gold nanoparticle approach for the targeted treatment of Pseudomonas aeruginosa biofilms

Abstract: Glycoconjugate-based AuNPs were developed for the targeted eradication of P. aeruginosa. This was achieved through carbohydrate-based recognition of key virulence factors followed by combined therapeutic delivery and light-based treatment (PDT/PTT).

Cited by 26 publications

References 55 publications

Metabolically Specific In Situ Fluorescent Visualization of Bacterial Infection on Wound Tissues

Metabolically Specific In Situ Fluorescent Visualization of Bacterial Infection on Wound Tissues

Recent Developments in the Use of Glyconanoparticles and Related Quantum Dots for the Detection of Lectins, Viruses, Bacteria and Cancer Cells

Advances in Nanoparticle Drug Delivery Systems for Anti-Hepatitis B Virus Therapy: A Narrative Review

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