A growth factor from neuronal cell lines stimulates myelin protein synthesis in mammalian brain

Abstract

Oligodendroglia growth factor (OGF) is a 16-kDa soluble protein produced by neuronal cell lines. This factor, when incubated with brain glia in culture, selectively stimulates growth of oligodendroglia, the myelin-producing cells of the CNS. OGF infused into the cerebral cortex of the adult rat accelerates the production of myelin proteins as shown by increased specific activity of the myelin enzyme 2′,3′-cyclic nucleotide 3′-phosphohydrolase (2′,3′-CNPase), by stimulated synthesis of myelin basic protein, and by elevations in levels of myelin proteolipid protein RNA. The ability of OGF to induce myelin protein production in vivo suggests that neuron-secreted growth factors help to regulate myelin formation within the CNS.

GM1-gangliosidosis in Alaskan Huskies: Clinical and Pathologic Findings

“…Corresponding age-, size-, and sex-matched dogs (Nos. [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] were used as controls. Samples of various tissues, including brain, spinal cord, eye, liver, kidney, urinary bladder, spleen, lymph nodes, lung, heart, intestine, pancreas, salivary gland, adrenal gland, thyroid gland, bones, bone marrow, and skin, were collected during necropsy and fixed in 10% formalin.…”

“…1-3 and controls (Nos. [10][11][12][13][14][15][16][17][18][19] were incubated with primary antibodies directed against glial fibrillary acidic protein (GFAP), myelin basic protein (MBP), neurofilament (NF), and major histocompatibility complex class II (MHC II) antigen employing the avidin-biotin-peroxidase complex method as previously described. 10,18,21 Sections (3 m thick) were deparaffinized in xylene and rehydrated through graded alcohols.…”

Differential expression in glial cells derived from chick embryo cerebral hemispheres at an advanced stage of development

“…Some information is beginning to accumulate as to the possible role of neuronal factors on astrocyte differentiation. Giulian et al (1985Giulian et al ( , 1991 have described the isolation of several different glia-promoting factors (GPFs) found in the brain which selectively stimulate growth of either cultured astrocytes or oligodendrocytes. Some of these protein factors are produced by neurons (Giulian and Young, 1986;Bottenstein et al, 1988).…”

Stem Cell Therapy in Pediatric Neurological Disabilities

“…Stem cells differentiate into neurons, oligodendrocytes and astrocytes which replace and repair the white matter injury in CP [17] (Figure 2). The growth factors secreted by these cells also help in remyelination, synaptogenesis, cytoprotection and angiogenesis which reverse the cellular injury in CP [18,19]. Numerous preclinical studies have In 2015, we published a nonrandomized study demonstrating the benefits of autologous bone marrow mononuclear cells (BMMNCs) in cerebral palsy [27].…”