A high density genetic map of tobacco (Nicotiana tabacum L.) obtained from large scale microsatellite marker development

Abstract

Tobacco (Nicotiana tabacum L.) is a species in the large family of the Solanaceae and is important as an agronomic crop and as a model system in plant biotechnology. Despite its importance, only limited molecular marker resources are available that can be used for genome analysis, genetic mapping and breeding. We report here on the development and characterization of 5,119 new and functional microsatellite markers and on the generation of a high-resolution genetic map for the tetraploid tobacco genome. The genetic map was generated using an F2 mapping population derived from the intervarietal cross of Hicks Broadleaf × Red Russian and merges the polymorphic markers from this new set with those from a smaller set previously used to produce a lower density map. The genetic map described here contains 2,317 microsatellite markers and 2,363 loci, resulting in an average distance between mapped microsatellite markers which is less than 2 million base pairs or 1.5 cM. With this new and expanded marker resource, a sufficient number of markers are now available for multiple applications ranging from tobacco breeding to comparative genome analysis. The genetic map of tobacco is now comparable in marker density and resolution with the best characterized genomes of the Solanaceae: tomato and potato.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-011-1578-8) contains supplementary material, which is available to authorized users.

Cell-to-cell movement of mitochondria in plants

“…Location of the SSR markers on the N. tabacum chromosomes is described in ref. 38. The PCR program of 94°C for 5 min; 37 cycles of 94°C for 45 s, 59°C for 45 s, 72°C for 1 min; 72°C for 10 min was used for all but chromosomes 8, 12, 14, and 16.…”

Mixed linear model association mapping for low chloride accumulation rate in oriental-type tobacco (Nicotiana tabaccum L.) germplasm

“…Twenty-six SSR primer pairs out of 278 from the tobacco SSR database (Bindler et al 2007(Bindler et al , 2011 were used for DNA fingerprinting. The choice of SSR markers was based on their known genetic locations to obtain near-uniform coverage of the tobacco genome and clarity of produced bands (Bindler et al 2007(Bindler et al , 2011. PCR was performed in a 20 µl volume using a 96-well Eppendorf Mastercycler Gradient (Type 5331, Eppendorf AG, Hamburg, Germany).…”

Characterization of hybrid seedlings from crosses of <i>Nicotiana stocktonii</i> Brandegee×<i>N. tabacum</i> L. and <i>N. stocktonii</i>×progenitors of <i>N. tabacum</i>

“…tabacum did not express lethal symptoms in this study. Recently, the first linkage map for N. tabacum was constructed by Bindler et al (2011) with SSR markers. In the N. tabacum genome, the S genome and T genome did not double simply and several types of variation occurred.…”