A hybrid system of carbon ink electrodes and chromatography paper on a CMOS chip and its application to enzymatic glucose sensor

Miki, Masashi; Iwahara, S.; Uno, Shigeyasu

doi:10.7567/jjap.53.04el06

Cited by 4 publications

(3 citation statements)

References 51 publications

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“…The use of carbon ink is prevalent and widely employed in screen printed or ink-jet printed disposable biochemical sensors (4,5). Our first use of carbon ink on CMOS metal pads showed stable and good electrochemical performance (6). Here, the WE and CE were fabricated with carbon-ink (Jelcon CH-8, Jujo Chemical) and the RE was formed by Ag/AgCl ink.…”

Section: Amperometric and Voltammetric Methods On Cmos Lsi Chipmentioning

confidence: 99%

(Invited)Electrochemical Biosensors Based on CMOS LSI Chips

Uno
¹

2018

ECS Trans.

0
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We have been working on electrochemical biosensing using CMOS chip as a platform. Three major electrochemical methods, amperometric (voltammetric), potentiometric, and impedance methods, are aimed to be done by electrodes on a CMOS chip. In amperometric measurement, carbon-ink is used to form electrochemically-stable electrodes instead of expensive gold electrode formation by photolithography. Enzymes and mediator (redox) molecules are immobilized in pieces of chromatography paper, and layers of such paper are placed directly on CMOS chip. Both amperometric and potentiometric detection of glucose concentration have been demonstrated. We have also been working on computer simulation and modeling of the three electrochemical measurements done by microscale electrodes on CMOS chip. Examples include 3D amperometric current simulation of 2D microelectrode array on a CMOS chip, potentiometric measurement of pH and DNA on an ion-sensitive field-effect transistor, as well as living cell monitoring based on impedance method. We also design CMOS integrated circuits for on-chip biosensing based on the electrochemical methods.

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Section: Amperometric and Voltammetric Methods On Cmos Lsi Chipmentioning
confidence: 99%

(Invited)Electrochemical Biosensors Based on CMOS LSI Chips
Uno
¹

2018
ECS Trans.
0
0
0
0
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“…The LOD was 0.11 mM and linear range was 1–10 mM. Miki et al [121] replaced screen-printed electrode with complementary metal–oxide–semiconductor (CMOS) chips for electrochemical paper-based glucose detection. Electrodes were fabricated on CMOS chips, the working electrode (WE) and counter electrode (CE) were dropped with carbon ink, and the reference electrode (RE) was formed using Ag/AgCl ink.…”

Section: Electrochemical Detection Of Glucosementioning
confidence: 99%

A Review on Microfluidic Paper-Based Analytical Devices for Glucose Detection
Liu
¹
,
Su
²
,
Li
³

2016
Sensors
114
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72
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Glucose, as an essential substance directly involved in metabolic processes, is closely related to the occurrence of various diseases such as glucose metabolism disorders and islet cell carcinoma. Therefore, it is crucial to develop sensitive, accurate, rapid, and cost effective methods for frequent and convenient detections of glucose. Microfluidic Paper-based Analytical Devices (μPADs) not only satisfying the above requirements but also occupying the advantages of portability and minimal sample consumption, have exhibited great potential in the field of glucose detection. This article reviews and summarizes the most recent improvements in glucose detection in two aspects of colorimetric and electrochemical μPADs. The progressive techniques for fabricating channels on μPADs are also emphasized in this article. With the growth of diabetes and other glucose indication diseases in the underdeveloped and developing countries, low-cost and reliably commercial μPADs for glucose detection will be in unprecedentedly demand.

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“…Electrochemical biosensors which utilize immobilized oxidase [1][2][3][4][5][6] or metal/metal oxide [7][8][9][10] to catalyze the oxidation of target analyte, could respond to the changes of concentration of the analyte, and output electrical signal with some disciplines. It can be used to determine biological, clinical, or environmental substances.…”

mentioning
confidence: 99%

Self-Assembling of Electrochemical Glucose Biosensor with Bacteriostatic Materials via Layer-by-Layer Method
Gao
¹
,
Luo
²
,
Zhang
³

et al. 2017
J. Electrochem. Soc.
8
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4
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Poly-diallyl-dimethyl-ammonium chloride (PDDA) solution was used to disperse carbon nanotubes (CNTs) to form a stable PDDACNTs aqueous dispersion. The negatively charged glucose oxidase (GOx) and positively charged PDDA-CNTs composite were used to prepare multilayer biosensing films on glassy carbon electrodes (GCE) via layer-by-layer (LBL) self-assembly technique. The optimum number of layers on GCE was 4. A mixture of 3-dimethyl (methacryloyloxyethyl) ammonium propane sulfonate (DMAPS) and Graphene (GR) was dropped on the multilayer films to prepare a bacteriostatic glucose biosensor. The results show that CNTs could evenly disperse in the PDDA films and the multilayer PDDA-CNTs films can significantly improve the catalytic current response toward glucose (Glu). The biosensor could detect glucose linearly from 16.5 to 214. Electrochemical biosensors which utilize immobilized oxidase [1][2][3][4][5][6] or metal/metal oxide 7-10 to catalyze the oxidation of target analyte, could respond to the changes of concentration of the analyte, and output electrical signal with some disciplines. It can be used to determine biological, clinical, or environmental substances.11-13 Many works have been carried out to achieve high biosensing performance. 14-17However, less research has been done about the bacteriostatic electrochemical glucose biosensor.Antimicrobial or bacteriostatic material has been widely used in industries such as textile, food fermentation and medical device industry. 18,19 In the process of bio-fermentation, the detection of various biochemical parameters (biomass, cell activity, substrate, nutrition, products and metabolites) forms the basis of controlling process of the fermentation. 20 The growth rate of micro-organism can be monitored by the consumption of glucose. Consequently, the abnormal phenomenon of fermentation process can be forecasted by the timely detection of glucose.21 However, bacterium can be easily adsorbed on the surface of the glucose sensors to form a bio-film. The bio-film can block the substrate close to the electrode, and the accuracy of the detection can be affected. 22 In these cases, it is especially necessary to use antimicrobial or bacteriostatic glucose biosensors. But there are very few studies reporting the antimicrobial or bacteriostatic electrochemical glucose biosensor. In this paper, a bacteriostatic film is built on GCE via layer-by-layer (LbL) self-assembly method and used to construct a glucose sensor.It is generally known that chemical compounds contain quaternized ammonium groups and that zwitterionic sulfopropylbetaine shows bacteriostatic or antimicrobial properties, 23 besides the zwitterionic materials show outstanding protein resistance performance. 24,25 A typical sulfobetaine, 3-dimethyl(methacryloyloxyethyl) ammonium propane sulfonate (DMAPS) (Scheme 1), which contains a sulfonate group and a quarternized ammonium separated by an alkyl spacer, has outstanding antimicrobial properties.Layer-by-layer (LbL) self-assembly method is a useful and versatile tech...

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A hybrid system of carbon ink electrodes and chromatography paper on a CMOS chip and its application to enzymatic glucose sensor

Cited by 4 publications

References 51 publications

(Invited)Electrochemical Biosensors Based on CMOS LSI Chips

(Invited)Electrochemical Biosensors Based on CMOS LSI Chips

A Review on Microfluidic Paper-Based Analytical Devices for Glucose Detection

Self-Assembling of Electrochemical Glucose Biosensor with Bacteriostatic Materials via Layer-by-Layer Method

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