A metagenomic comparison of endemic viruses from broiler chickens with runting-stunting syndrome and from normal birds

Devaney, Ryan; Trudgett, James; Trudgett, Alan; Meharg, Caroline; Smyth, Victoria

doi:10.1080/03079457.2016.1193123

Cited by 54 publications

(54 citation statements)

References 110 publications

(99 reference statements)

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“…Chicken astrovirus, parvovirus, calicivirus and rotavirus have been identified as causes of gastrointestinal tract infections in poultry, such as RSS, which is also called malabsorption syndrome [27]. A few studies using viral metagenomics could not specify any particular pathogen from the viral community in the chicken flocks with RSS because the distributions of enteric viruses in diseased and healthy flocks were not significantly different [17,28,29]. Chicken astrovirus has been distributed worldwide for decades and is nearly ubiquitous constituent of the chicken gut [30].…”

Section: Discussionmentioning

confidence: 99%

Viral metagenomic analysis of chickens with runting-stunting syndrome in the Republic of Korea

Kim

Kwon

Jang

et al. 2020

Virol J

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Background and aims: Runting-stunting syndrome (RSS) in chickens, also known as malabsorption syndrome, which is characterized by mild to severe enteritis and diagnosed through typical histopathologic examination as well as clinical signs, results in considerable economic losses. Despite the many studies carried out over decades to determine the etiologic agents of RSS involved in the disease, several outbreaks remained without the elucidation of, potentially multiple, etiologies involved. Methods: We performed comparative analysis of viral metagenomes from four chicken flocks affected with RSS using next-generation sequencing. Primers for the detection of chicken enteric viruses were designed from the sequencing data obtained with metagenomics. Multiplex reverse transcription-polymerase chain reaction (PCR) and PCR were performed to detect a variety of etiological agents previously described in natural cases of RSS.Results: The most abundant viral families identified in this study were Astroviridae, Picornaviridae, Parvoviridae, Caliciviridae, Reoviridae and Picobirnaviridae. Chicken astrovirus sequences were present in all four samples, suggesting an association between chicken astrovirus and RSS and chicken astrovirus as a candidate pathogen responsible for RSS. Picobirnavirus and the newly identified chapparvovirus were found in chickens in the Republic of Korea for the first time, and the genetic diversity of enteric viruses and viral communities was showed. Conclusions: Chicken astrovirus was consistently detected in broilers affected with RSS and the result of this study may contribute to knowledge of enteric diseases and viruses in chickens.

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Section: Discussionmentioning

confidence: 99%

Viral metagenomic analysis of chickens with runting-stunting syndrome in the Republic of Korea

Kim

Kwon

Jang

et al. 2020

Virol J

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“…Epidemiological investigations demonstrated wide distribution of parvoviruses in commercial chicken and turkey flocks in the United States and European countries including Hungary, Poland and Croatia Bidin et al, 2011;Domanska-Blicharz et al, 2012). Recent surveys indicated incidence of ChPV from Asia (South Korea and China), Brazil, Canada and United Kingdom (Fountain et al, 2011;Koo et al, 2013;Nuñez et al, 2015;Devaney et al, 2016;Feng et al, 2016). The details of prevalence of aveparvovirus with their health status, reported from different countries, is given in Table 2.…”

Section: Epidemiology and Transmissionmentioning

confidence: 99%

“…The presence of a parvoviral genome has also been reported in clinically healthy chicken and turkey flocks (2-17 weeks old). Occurrence of these viruses in the healthy avian gut and its association is unclear (Domanska-Blicharz et al, 2012Devaney et al, 2016;Lima et al, 2017). Histologically, the small intestine has acute catarrhal enteritis and moderate to severe distension of crypts with associated eosinophilic intranuclear inclusions within epithelial cells.…”

Section: Clinical Features Pathology and Pathogenesismentioning

confidence: 99%

“…Sequence-independent techniques, such as next generation sequencing, have allowed the detection of avian viral genome diversity from healthy and affected birds Kapgate et al, 2015). The comparative metagenomics studies revealed ChPV genomic sequence diversity identified from RSSaffected and unaffected broilers (Devaney et al, 2016;Lima et al, 2017), and divergent turkey parvovirus sequences from turkey faeces (Reuter et al, 2014). The characterization of the virome will provide a baseline for future studies comparing viral populations in healthy and diseased flocks.…”

Section: Diagnosismentioning

confidence: 99%

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Avian parvovirus: classification, phylogeny, pathogenesis and diagnosis

et al. 2018

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Poultry parvoviruses identified during the early 1980s are found worldwide in intestines from young birds with enteric disease syndromes as well as healthy birds. The chicken parvovirus (ChPV) and turkey parvovirus (TuPV) belong to the Aveparvovirus genus within the subfamily Parvovirinae. Poultry parvoviruses are small, non-enveloped, single-stranded DNA viruses consisting of three open reading frames, the first two encoding the non-structural protein (NS) and nuclear phosphoprotein (NP) and the third encoding the viral capsid proteins 1 (VP1 and VP2). In contrast to other parvoviruses, the VP1-unique region does not contain the phospholipase A2 sequence motif. Recent experimental studies suggested the parvoviruses to be the candidate pathogens in cases of enteric disease syndrome. Current diagnostic methods for poultry parvovirus detection include PCR, real-time PCR, enzyme linked immunosorbent assay using recombinant VP2 or VP1 capsid proteins. Moreover, sequenceindependent amplification techniques combined with next-generation sequencing platforms have allowed rapid and simultaneous detection of the parvovirus from affected and healthy birds. There is no commercial vaccine; hence, the development of an effective vaccine to control the spread of infection should be of primary importance. This review presents the current knowledge on poultry parvoviruses with emphasis on taxonomy, phylogenetic relationship, genomic analysis, epidemiology, pathogenesis and diagnostic methods. ARTICLE HISTORY

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“…The primary aetiology of RSS or MAS in chickens is still unknown, although several viruses have been identified in birds with RSS, and ChPV being found in many of these disorders (Goodwin et al, 1993;Pantin-Jackwood et al, 2008;Domanska-Blicharz et al, 2012;Devaney et al, 2016). ChPV has a worldwide distribution, and it has been associated with enteric diseases in many other countries (Kisary et al, 1984;Decaesstecker et al, 1986;Goodwin et al, 1990;Zsak et al, 2008Zsak et al, ,2009Bidin et al, 2011;Domanska-Blicharz et al, 2012;Tarasiuk et al, 2012;Nuñez et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Molecular Diagnostic of Chicken Parvovirus (ChPV) Affecting Broiler Flocks in Ecuador

Torre

Núñez

Puga

et al. 2018

Braz. J. Poult. Sci.

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Enteric diseases affect poultry and cause important economic losses in many countries worldwide. Avian parvovirus has been linked to enteric conditions, such as malabsorption and runting-stunting syndrome (RSS), characterized by diarrhoea, and reduced weight gain and growth retardation. In 2013 and 2016, 79 samples were collected from different organs of chickens in Ecuador that exhibited signs of diarrhea and stunting syndrome, and analysed for the presence of chicken parvovirus (ChPV). The detection method of ChPV applied was Polymerase Chain Reaction (PCR), using primers designed from the conserved region of the viral genome that encodes the non-structural protein NS1. Out of the 79 samples, 50.6% (40/79) were positive for ChPV, and their nucleotide and amino acid sequences were analysed to determine their phylogenetic relationship with the sequences reported in the United States,

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A metagenomic comparison of endemic viruses from broiler chickens with runting-stunting syndrome and from normal birds

Cited by 54 publications

References 110 publications

Viral metagenomic analysis of chickens with runting-stunting syndrome in the Republic of Korea

Viral metagenomic analysis of chickens with runting-stunting syndrome in the Republic of Korea

Avian parvovirus: classification, phylogeny, pathogenesis and diagnosis

Molecular Diagnostic of Chicken Parvovirus (ChPV) Affecting Broiler Flocks in Ecuador

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