This case study examines seed colour during ripening using an exhaustive colorimetric analysis. The aim was to associate the chemical ripening with the seed colour in order to estimate the ripening stages of Cabernet Sauvignon grapes. Cluster samples, obtained from a vertical shoot-positioned vineyard in central Chile, were monitored for their technological and phenolic maturity, as well as for the colorimetric and chemical characteristics of the seeds. The colours of the scanned seed images were determined by human as well as by computer vision. In the first case, an expert assigned a colour to each seed part. In the second case, a computer program estimated the colours of the scanned seed images. An exhaustive analysis of seed colour was proposed, instead of a general observation of seed browning. The seed colour presented a wide range of colours, from moss green to dark brown, depending on the maturity and the face observed. The ripening stages identified, along with the chemical and colorimetric information gathered, were under ripe seed (brown with green traces), ripe seed (dark brown with green traces) and overripe seed (dark brown without any green traces). A new way to quantify seed colour is shown in this paper.