A Methylene Blue Assisted Electrochemical Sensor for Determination of Drug Resistance of Escherichia coli

Abstract

Due to the abuse of antibiotics in clinical, animal husbandry, and aquaculture, drug-resistant pathogens are produced, which poses a great threat to human and the public health. At present, a rapid and effective drug sensitivity test method is urgently needed to effectively control the spread of drug-resistant bacteria. Using methylene blue as a redox probe, the electrochemical signals of methylene blue in drug-resistant Escherichia coli strains were analyzed by a CV method. Graphene ink has been used for enhancing the electrochemical signal. Compared with the results of the traditional drug sensitivity test, we proposed a rapid electrochemical drug sensitivity test method which can effectively identify the drug sensitivity of Escherichia coli. The sensitivity of four E. coli isolates to ciprofloxacin, gentamicin, and ampicillin was tested by an electrochemical drug sensitivity test. The respiratory activity value %RA was used as an indicator of bacterial resistance by electrochemical method.

Revolutionizing Phenotypic Antimicrobial Susceptibility Testing: Lightning-Fast Techniques Based on Cutting-Edge Electrochemistry

“…Rongshuai Duan et. al 33 presents a study showcasing the detection of resistant Escherichia coli bacteria by employing Methylene Blue as an electron acceptor to observe bacterial respiration. Additionally, they utilized graphene ink to augment the electrochemical response for enhanced detection.…”

Electrochemical antibiotic susceptibility testing: An emerging approach for fast and accurate determination of antibiotic effect in complex samples

“…Approaches akin to microbial fuel cell systems have also been reported where measurement of the total accumulated energy and its correlation with gold standard MIC assays was demonstrated using the common pathogen Pseudomonas aeruginosa [24]. Methylene blue has been used as a redox probe to measure microbial metabolism and in the process showing that sensitivity of four E. coli isolates to ciprofloxacin, gentamicin, and ampicillin could be readily established [25]. In addition to these methods highlighted, several other studies have reported viable routes to determining AST in a phenotypic manner [26]- [33].…”

Electrochemical Biosensing Interface Based on Carbon Dots-Fe3O4 Nanomaterial for the Determination of Escherichia coli O157:H7

“…In recent years, food poisoning caused by foodborne pathogens has frequently occurred worldwide, which is the most prominent public health problem in the world (Hou et al, 2020;Duan et al, 2021). As one of the most hazardous foodborne pathogens, Escherichia coli (E. coli) O157:H7 can cause some diseases such as hemorrhagic colitis and hemolytic uremic syndrome.…”