A Micro Method for the Ultraviolet Spectrophotometric Determination of Theophylline1

Hohnadel, David C.; Grove, Thomas H.; Alonzo, Perlita

doi:10.1093/jat/2.4.141

Cited by 4 publications

(3 citation statements)

References 20 publications

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“…Prior to detection, the accuracy of the RNA aptasensor was tested in 50 mM HEPES buffer with 150 mM NaCl and 1 mM MgCl 2 by comparing it with a classical theophylline-determination method (UV-vis spectrophotometry) (Jatlow, 1975;Hohnadel et al, 1978). The absorbance of the theophylline sample was determined at 275 nm (A 275 ) and 300 nm (A 300 ; detailed steps are provided in the Supplementary materials).…”

Section: Detection Of Theophylline In Serummentioning

confidence: 99%

A self-assembling RNA aptamer-based graphene oxide sensor for the turn-on detection of theophylline in serum

Ling¹,

Jiang

et al. 2016

Biosensors and Bioelectronics

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Section: Detection Of Theophylline In Serummentioning

confidence: 99%

A self-assembling RNA aptamer-based graphene oxide sensor for the turn-on detection of theophylline in serum

Ling¹,

Jiang

et al. 2016

Biosensors and Bioelectronics

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“…Whereas for levosalbutamol sulfate a few spectrophotometric Thulasama et al, 2009) and HPLC (Ghemud et al, 2012;Halabi et al, 2004; methods are reported. The reported methods for theophylline includes spectrophotometric (Abuirjeie et al, 1992;Bermejo et al, 1985;Carter et al, 1985;Fellenberg et al, 1979;Hohnadel et al, 1978;Jatlow, 1975;Kumar et al, 2011;Plavsic, 1978;Vasiliades et al, 1976), HPLC (Abuirjeie et al, 1992;Boberic-Borojevic et al, 1999;Greenberg et al, 1979;Lauff, 1987;Moncrieff, 1991;Papadoyannis et al, 1993;Shidhaye et al, 2009), GC (Chrzanowski et al, 1974;Schwertner, 1979;Shah et al, 1974) and LC-MS (Abdel-Hamid et al, 2003;Babu et al, 2011;Kanazawa et al, 2000;Song et al, 2004). The literature survey suggests there is lack of a stability indicating RP-HPLC method for simultaneous determination of levosalbutamol sulfate and theophylline in combined syrup dosage forms.…”

Section: Introductionmentioning

confidence: 99%

Stability-indicating RP-HPLC method for simultaneous estimation of levosalbutamol sulfate and theophylline in combined dosage form

Panda

Kumar

Mohanta

2013

Braz. J. Pharm. Sci.

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A novel, simple, accurate and precise RP-HPLC method for simultaneous determination of levosalbutamol sulfate and theophylline has been developed and validated. Separation was achieved on a Phenomenex; C 18 column (250 mm × 4.6 mm i.d., 5 μm) using methanol: 10 mM TBAHS(tetrabutyl ammonium hydrogen sulfate) (50:50, v/v) as mobile phase at flow rate of 1.0 mL.min -1 . The UV detection wavelength was 274 nm. The linearity is obeyed over a concentration range of 0.5-150 μg.mL -1 with correlation coefficient of 0.999 for both the drugs. The proposed method was validated by determining accuracy, precision, stability and system suitability parameters. The method was found to be robust. Specificity of the method was determined by subjecting the drugs to various stress conditions like acid, alkali, oxidation, thermal and photolytic degradation. The method was used successfully for the simultaneous determination of levosalbutamol sulfate and theophylline in syrup dosage form.Uniterms: Levosalbutamol/determination. Theophylline/determination. Stability-indicating. RP-High performance liquid chromatography/qualitative analysis Syrup/qualitative analysis.Desenvolveu-se e validou-se método de RP-HPLC novo, simples, exato e preciso de determinação simultânea do sulfato de levossalbutamol e teofilina.. A separação foi efetuada em uma coluna Phenomenex; C18 (250 mm x 4,6 mm d.i., 5 μm) utilizando metanol: TBAHS (hidrogenossulfato de tetrabutilamônio) 10 mM (50:50, v/v) como fase móvel, com fluxo de 1,0 mL.min -1 . O comprimento de onda de detecção no UV foi 274 nm. Observou-se linearidade na faixa de concentração de 0,5-150 μg mL -1 , com coeficiente de correlação de 0,999 para ambos os fármacos. O método proposto foi validado determinando-se exatidão, precisão, estabilidade e parâmetros de adequação do sistema. O método mostrou-se robusto. A especificidade do método foi determinada submetendo os fármacos a várias condições de estresse, como ácido, álcali, oxidação, degradação térmica e fotolítica. O método foi usado com sucesso para a determinação simultânea do sulfato de levossalbutamol e teofilina na forma de xarope.

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“…Several methods are available for the quantification of theophylline in serum. T h e original method used ultraviolet (UV) spectroscopy (6), but although improved in later years (7,8), this method still exhibits unacceptable interference by other drugs (9). Consequently, many alternative methods have been reported.…”

Section: Introductionmentioning

confidence: 99%

High-Performance Liquid Chromatographic Analysis of Theophylline in Serum and Its Use in Therapeutic Drug Monitoring

El–Sayed

Islam

1989

J Clin Pharm Ther

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A rapid, highly sensitive high-performance liquid chromatographic method has been developed for the determination of theophylline in serum using beta-hydroxyethyltheophylline as an internal standard (IS). Theophylline and IS were extracted from serum using a mixture of dichloromethane: isopropanol (90: 10, v/v) and eluted from a 5 microns, C-18 reversed-phase column at 60 degrees C with a mobile phase consisting of sodium acetate buffer-acetonitrile (90:10, v/v), at a flow rate of 1 ml/min with ultraviolet detection at 280 nm. Each analysis required no longer than 7 min to perform. Quantification was achieved by the measurement of the peak: height ratio and the relative and absolute recoveries varied from 86 to 100%. Within-day coefficients of variation ranged from 2.9 to 5.1% and between-day coefficients of variation from 1.1 to 5.9% in subtherapeutic, therapeutic, and toxic concentrations.

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A Micro Method for the Ultraviolet Spectrophotometric Determination of Theophylline1

Cited by 4 publications

References 20 publications

A self-assembling RNA aptamer-based graphene oxide sensor for the turn-on detection of theophylline in serum

A self-assembling RNA aptamer-based graphene oxide sensor for the turn-on detection of theophylline in serum

Stability-indicating RP-HPLC method for simultaneous estimation of levosalbutamol sulfate and theophylline in combined dosage form

High-Performance Liquid Chromatographic Analysis of Theophylline in Serum and Its Use in Therapeutic Drug Monitoring

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