A microfluidic nano-biosensor for the detection of pathogenic Salmonella

Kim, Giyoung; Moon, Ji-Hea; Moh, Chang-Yeon; Lim, Jongguk

doi:10.1016/j.bios.2014.08.023

Cited by 155 publications

(66 citation statements)

References 33 publications

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“…Therefore, glucose could be reacted to form more hydrogen peroxide and gluconic acid, and more hydrogen ions were futher produced. Because of increment of the hydrogen ion, the sensitivity of the glucose biosensor was also changed [14], [23], [27]. With respect to the diffusion resistance, it consisted of friction and viscous force, which existed between the sensing membrane and the solution.…”

Section: A Characteristics Of Arrayed Flexible Glucose Biosensor Modmentioning

confidence: 99%

Dynamic and Wireless Sensing Measurements of Potentiometric Glucose Biosensor Based on Graphene and Magnetic Beads

et al. 2015

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In this paper, the arrayed flexible pH sensor and glucose biosensor modified by magnetic beads and graphene were proposed. The ruthenium dioxide (RuO 2 ) sensing films were deposited by radio frequency sputtering system and the screenprinted technique was used to construct the silver conducting wires and insulation layer of the RuO 2 electrodes. In order to enhance performance of the pH sensor and glucose biosensor, the microfluidic device had been utilized and developed. In the measurement processes, the different pH and glucose solutions were investigated in various flow rates. According to the experimental results, the average sensitivity of the pH sensor was enhanced from 52.280 to 57.981 mV/pH and the average sensitivity of the RuO 2 /graphene/magnetic bead-GO x -Nafion glucose biosensor was enhanced from 10.628 to 13.541 mV/mM. With regard to the wireless sensing measurements, the wireless sensing system which complied the ZigBee standard was employed to transmit the signals of the pH or glucose measurements in this investigation, and the system consisted of the Xbee device, Arduino Mega 2560, readout circuit, pH or glucose biosensor and computer. According to the experimental results, the average sensitivity and linearity of the pH sensor were 51.063 mV/pH and 0.988, respectively, and the average sensitivity and linearity of the RuO 2 /graphene/magnetic bead-GO x -Nafion glucose biosensor were 11.005 mV/mM and 0.995, respectively.

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Section: A Characteristics Of Arrayed Flexible Glucose Biosensor Modmentioning

confidence: 99%

Dynamic and Wireless Sensing Measurements of Potentiometric Glucose Biosensor Based on Graphene and Magnetic Beads

et al. 2015

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“…[78] More recently these biosensors are being integrated into other type of structures such microcircuits and microfluidic devices. [79] [80] Although these technologies currently have low sensitivities, they are promising detection methods since they consist of inexpensive, integrated microcircuit that is easy to use.…”

Section: Introductionmentioning

confidence: 99%

Nanotechnology to the rescue: using nano-enabled approaches in microbiological food safety and quality

Eleftheriadou

Pyrgiotakis²,

Demokritou³

2017

Current Opinion in Biotechnology

140

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Food safety and quality assurance is entering a new era. Interventions along the food supply chain must become more efficient in safeguarding public health and the environment and must address numerous challenges and new consumption trends. Current methods of microbial control to assure the safety of food and minimize microbial spoilage have each shown inefficiencies. Nanotechnology is a rapidly expanding area in the agri/feed/food sector. Nano-enabled approaches such as antimicrobial food-contact surfaces/packaging, nano-enabled sensors for rapid pathogen/contaminant detection and nano-delivered biocidal methods, currently on the market or at a developmental stage, show great potential for the food industry. Concerns on potential risks to human health and the environment posed by use of engineered nanomaterials (ENMs) in food applications must, however, be adequately evaluated at the developmental stage to ensure consumer's acceptance.

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“…Nevertheless, in‐line IMS with on‐chip cultivation and quantification by bed expansion, which can be easily followed with a low‐end camera, was recently demonstrated in this chip . Other detection techniques integrated with the microfluidic immunocapture of bacteria were reported over the past few years, such as fluorescent dyes or quantum dots‐based immunoassays , light scattering , surface‐plasmon resonance , or PCR‐based techniques.…”

Section: Resultsmentioning

confidence: 99%

“…There is still a need for the development of such portable devices for the routine and rapid monitoring of food quality in order to prevent massive foodborne pathogen outbreaks . This effort is supported by the recent increase in published papers dealing with the rapid biosensor‐based immunocapture and detection of foodborne pathogens .…”

Section: Introductionmentioning

confidence: 99%

Advanced immunocapture of milk‐borne Salmonella by microfluidic magnetically stabilized fluidized bed

et al. 2017

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The success of microfluidic immunocapture based on magnetic beads depends primarily on a sophisticated microscale separation system and on the quality of the magnetic immunosorbent. A microfluidic chip containing a magnetically stabilized fluidized bed (μMSFB), developed for the capture and on‐chip amplification of bacteria, was recently described by Pereiro et al.. The present work shows the thorough development of anti‐Salmonella magnetic immunosorbents with the optimal capture efficiency and selectivity. Based on the corresponding ISO standards, these parameters have to be high enough to capture even a few cells of bacteria in a proper aliquot of sample, e.g. milk. The selection of specific anti‐Salmonella IgG molecules and the conditions for covalent bonding were the key steps in preparing an immunosorbent of the desired quality. The protocol for immunocapturing was first thoroughly optimized and studied in a batchwise arrangement, and then the carrier was integrated into the μMSFB chip. The combination of the unique design of the chip (guaranteeing the collision of cells with magnetic beads) with the advanced immunosorbent led to a Salmonella cell capture efficiency of up to 99%. These high values were achieved repeatedly even in samples of milk differing in fat content. The rate of nonspecific capture of Escherichia coli (i.e. the negative control) was only 2%.

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A microfluidic nano-biosensor for the detection of pathogenic Salmonella

Cited by 155 publications

References 33 publications

Dynamic and Wireless Sensing Measurements of Potentiometric Glucose Biosensor Based on Graphene and Magnetic Beads

Dynamic and Wireless Sensing Measurements of Potentiometric Glucose Biosensor Based on Graphene and Magnetic Beads

Nanotechnology to the rescue: using nano-enabled approaches in microbiological food safety and quality

Advanced immunocapture of milk‐borne Salmonella by microfluidic magnetically stabilized fluidized bed

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