This article deals with an original method to estimate the noise introduced by optical imaging systems, such as CCD cameras. The power of the signal-dependent photon noise is decoupled from the power of the signal-independent electronic noise. The method relies on the multivariate regression of sample mean and variance. Statistically similar image pixels, not necessarily connected, produce scatterpoints that are clustered along a straight line, whose slope and intercept measure the signal-dependent and signal-independent components of the noise power, respectively. Experimental results carried out on a simulated noisy image and on true data from a commercial CCD camera highlight the accuracy of the proposed method and its applicability to separate R-G-B components that have been corrected for the nonlinear effects of the camera response function, but not yet interpolated to the the full size of the mosaiced R-G-B image.