A New Immunosensor for Serum Myeloperoxidase Based on Self-Assembly of Multi-Walled Carbon Nanotubes/Thionine/Gold Nanoparticles-Chitosan

Diao, Qi; Li, Yuan

doi:10.4028/www.scientific.net/amr.343-344.1207

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…The various composite films have been reported for the electrochemical determination of MPO levels in biological samples. Some sensors for the detection of MPO using such composite films [33][34][35] were shown in Table 1 (see Table S1 in SI for a more comprehensive view) and compared their electroanalytical performances in terms of detection limit, and linear range of 9 concentration. The utilization of nanomaterials offers excellent biocompatibility, conductive properties, high surface-to-volume ratio, high mechanical strength, and rapid functionalization to increase the analytical feature of MPO detection.…”

Section: Figmentioning

confidence: 99%

Electrochemical approaches based on micro- and nanomaterials for diagnosing oxidative stress

et al. 2023

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This review article comprehensively discusses the various electrochemical approaches for measuring and detecting oxidative stress biomarkers and enzymes, particularly reactive oxygen/ nitrogen species, highly reactive chemical molecules, which are the byproducts of normal aerobic metabolism and can oxidize cellular components such as DNA, lipids, and proteins. First, we address the latest research on the electrochemical determination of reactive oxygen species generating enzymes, followed by detection of oxidative stress biomarkers, and final determination of total antioxidant activity (endogenous and exogenous). Most electrochemical sensing platforms exploited the unique properties of micro and nanomaterials such as carbon nanomaterials, metal or metal oxide nanoparticles (NPs), conductive polymers and metal-nano compounds, which have been mainly used for enhancing the electrocatalytic response of sensors/biosensors. The performance of the electroanalytical device commonly measured by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) in terms of detection limit, sensitivity, and linear range of detection was also discussed. This article provides a comprehensive review of electrode fabrication, characterization and evaluation of their performances, which are assisting to design and manufacture an appropriate electrochemical (bio)sensor for medical and clinical applications. The key points such as accessibility, affordability, rapidity, low cost, and high sensitivity of the electrochemical sensing devices were also highlighted for the diagnosis of oxidative stress. Overall, this review brings a timely discussion on past and current approaches for developing electrochemical sensors and biosensors mainly based on micro and nanomaterials for the diagnosis of oxidative stress.

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Section: Figmentioning

confidence: 99%

Electrochemical approaches based on micro- and nanomaterials for diagnosing oxidative stress

et al. 2023

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“…For the sensitive detection of this enzyme, an electrochemical immunosensor was developed involving self-assembly of MWCNTs, THI, AuNPs and Chit multilayers onto GCEs. Anti-MPO was adsorbed on AuNPs, and HRP was employed both as blocking agent for non-specific binding and to amplify the electrochemical responses [65]. A disposable electrochemical MPO immunosensor was also fabricated using indium tin oxide electrode (ITO) modified with a film composed of poly( o -phenylenediamine) ( o PD), MWCNT, the ionic liquid 1-ethyl-3-methylimidazolium bromine ([EMIM]Br, IL), and adsorbed AuNPs.…”

Section: Electrochemical Immunosensors Involving Carbon Nanomaterimentioning

confidence: 99%

Diagnostics Strategies with Electrochemical Affinity Biosensors Using Carbon Nanomaterials as Electrode Modifiers

2016

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Early diagnosis is often the key to successful patient treatment and survival. The identification of various disease signaling biomarkers which reliably reflect normal and disease states in humans in biological fluids explain the burgeoning research field in developing new methodologies able to determine the target biomarkers in complex biological samples with the required sensitivity and selectivity and in a simple and rapid way. The unique advantages offered by electrochemical sensors together with the availability of high affinity and specific bioreceptors and their great capabilities in terms of sensitivity and stability imparted by nanostructuring the electrode surface with different carbon nanomaterials have led to the development of new electrochemical biosensing strategies that have flourished as interesting alternatives to conventional methodologies for clinical diagnostics. This paper briefly reviews the advantages of using carbon nanostructures and their hybrid nanocomposites as electrode modifiers to construct efficient electrochemical sensing platforms for diagnosis. The review provides an updated overview of some selected examples involving attractive amplification and biosensing approaches which have been applied to the determination of relevant genetic and protein diagnostics biomarkers.

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Recent Trends in Electrochemical Sensors for Vital Biomedical Markers Using Hybrid Nanostructured Materials

et al. 2020

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This work provides a succinct insight into the recent developments in electrochemical quantification of vital biomedical markers using hybrid metallic composite nanostructures. After a brief introduction to the biomarkers, five types of crucial biomarkers, which require timely and periodical monitoring, are shortlisted, namely, cancer, cardiac, inflammatory, diabetic and renal biomarkers. This review emphasizes the usage and advantages of hybrid nanostructured materials as the recognition matrices toward the detection of vital biomarkers. Different transduction methods (fluorescence, electrophoresis, chemiluminescence, electrochemiluminescence, surface plasmon resonance, surface‐enhanced Raman spectroscopy) reported for the biomarkers are discussed comprehensively to present an overview of the current research works. Recent advancements in the electrochemical (amperometric, voltammetric, and impedimetric) sensor systems constructed with metal nanoparticle‐derived hybrid composite nanostructures toward the selective detection of chosen vital biomarkers are specifically analyzed. It describes the challenges involved and the strategies reported for the development of selective, sensitive, and disposable electrochemical biosensors with the details of fabrication, functionalization, and applications of hybrid metallic composite nanostructures.

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A New Immunosensor for Serum Myeloperoxidase Based on Self-Assembly of Multi-Walled Carbon Nanotubes/Thionine/Gold Nanoparticles-Chitosan

Cited by 3 publications

References 10 publications

Electrochemical approaches based on micro- and nanomaterials for diagnosing oxidative stress

Electrochemical approaches based on micro- and nanomaterials for diagnosing oxidative stress

Diagnostics Strategies with Electrochemical Affinity Biosensors Using Carbon Nanomaterials as Electrode Modifiers

Recent Trends in Electrochemical Sensors for Vital Biomedical Markers Using Hybrid Nanostructured Materials

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