A new species of Tamarixia Mercet (Hymenoptera, Eulophidae), a parasitoid of Diaphorina communis Mathur (Hemiptera, Liviidae) in Bhutan

Om, Namgay; Yefremova, Z. A.; Yegorenkova, E. N.; Beattie, G. A. C.; Donovan, N. J.; Holford, Paul

doi:10.1016/j.aspen.2017.04.011

Cited by 5 publications

(5 citation statements)

References 38 publications

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“…Among the conserved regions, the ITS loci are useful diagnostic markers due to their high divergence levels between species but low levels of variation within a species [33,63]. The ITS regions have been used to study phylogenetic relationships and resolve taxonomic controversies in various insect species, including T. triozae, T. mercet, and T. drukyulensis [25,27]. In the current study, the ITS regions have been used to construct the phylogenetic tree with twenty-one (ITS1) and thirty-one (ITS2) reference sequences of Tamarixia reported from the different agro-ecological zones, including T. radiata sequence from China populations and one from Ecuador (Guayas).…”

Section: Discussionmentioning

confidence: 99%

“…In previous studies, mitochondrial genes have been used to analyze the genetic diversity and genetic differentiation of the Tamarixia species [24,25,27]. Proper assimilation and understanding of the arthropods' genetic variability found it essential to mitigate and improve its monitoring, which further facilitates the implementation of need-based management strategies.…”

Section: Discussionmentioning

confidence: 99%

“…The phylogenetic analysis of ITS1, ITS2, COI, and wsp sequences of different populations of T. radiata was conducted using MEGA-X [50]. For additional support and diversity, already reported ITS1 sequences of T. radiata populations from Bhutan, Mexico, Vietnam, and USA (Florida and Texas regions) had been used [25,27]. Meanwhile, sequences from Bhutan, Finland, India, Puerto Rico, Vietnam, and USA (Florida and Texas regions) were employed for ITS2 analysis of T. radiata populations [25,27].…”

Section: Sequence Composition and Phylogenetic Analysismentioning

confidence: 99%

“…For additional support and diversity, already reported ITS1 sequences of T. radiata populations from Bhutan, Mexico, Vietnam, and USA (Florida and Texas regions) had been used [25,27]. Meanwhile, sequences from Bhutan, Finland, India, Puerto Rico, Vietnam, and USA (Florida and Texas regions) were employed for ITS2 analysis of T. radiata populations [25,27]. For phylogenetic tree constructions, the General Time Reversible under the initial tree option of maximum parsimony method was used for the maximum likelihood phylogeny [51], while the Maximum Composite Likelihood method was considered for Neighbor-Joining topology [52].…”

Section: Sequence Composition and Phylogenetic Analysismentioning

confidence: 99%

“…Formerly, the genetic diversity of T. radiata from several countries has also been studied using DNA marker sequencing. These molecular approaches are rapid characterization tools for identifying genetic diversities or similarities among geographically isolated T. radiata populations [24][25][26][27]. Molecular markers consisting of the mitochondrial gene (COI) and internal transcribed spacer (ITS1 and ITS2) regions have been extensively used to investigate the genetic diversity and phylogenetic relationships between the insect-host and parasitoids [28][29][30].…”

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity of Tamarixia radiata Populations and Their Associated Endosymbiont Wolbachia Species from China

et al. 2021

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Tamarixia radiata is one of the established biocontrol pests against the major Asian citrus psyllid, Diaphorina citri, a vector of Candidatus Liberibacter that is a causal agent of citrus Huanglongbing (HLB) disease. Updated information and regional exploration on biocontrol pests are important elements for effective disease management strategies. In this study, the diversity and parasitism rate of T. radiata populations were evaluated. Due to the importance of the host–parasitoid relationship, the presence of Wolbachia as an endosymbiont was also investigated. The parasitism rate of various T. radiata populations from Ecuador and China ranged between 57.27% and 66.32%, respectively, with a non-significant emergence rate and a statistically similar sex ratio. Sequence analysis of ITS and COI from T. radiata populations was consistent with the morphological hypothesis that the collections represent a single species, whereas phylogeny of the wsp gene confirmed the presence of Wolbachia pipientis as an endosymbiont within T. radiata populations. Based on partial COI sequences, the maximum genetic diversity such as total haplotype diversity (Hd = 0.788), nucleotide, diversity (π = 0.2439), and average nucleotide difference (k = 171.844) was also estimated for different T. radiata populations. Furthermore, neutrality tests based on COI sequences indicated an overall contraction in T. radiata populations, whereas an expansion trend was observed in associated W. pipientis strains. This study clearly demonstrated the presence of genetically diverse T. radiata populations that were able to parasitize D. citri effectively, and these can be further explored as promising biocontrol candidates in integrated pest management strategies to solve citriculture economic loss caused by D. citri.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Sequence Composition and Phylogenetic Analysismentioning

confidence: 99%

Section: Sequence Composition and Phylogenetic Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity of Tamarixia radiata Populations and Their Associated Endosymbiont Wolbachia Species from China

et al. 2021

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Incidence of psyllids and ‘Candidatus Liberibacter asiaticus’ in mandarin orchards at different altitudes in Tsirang, Bhutan

Beattie

Donovan

et al. 2021

Trop. plant pathol.

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A One-Step Multiplex PCR Method to Rapidly Distinguish Two Strains of Diglyphus wani (Hymenoptera: Eulophidae) Against Agromyzid Leafminers

Guo

et al. 2023

Journal of Economic Entomology

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Hymenopteran parasitoids generally show a haplo-diploid sex determination system. Haploid males are produced from unfertilized eggs, whereas diploid females develop from fertilized eggs (arrhenotokous). In some cases, diploid females develop from unfertilized eggs (thelytokous). Diglyphus wani (Hymenoptera: Eulophidae) is a biological control agent for agromyzid leafminers and have arrhenotokous and thelytokous strains. However, the morphological characteristics of two strains of D. wani are so similar that it is difficult to accurately distinguish them based on morphology. Here, a rapid molecular identification method was developed based on the mitochondrial gene cytochrome c oxidase I (COI) and one-step multiplex PCR. Two primer combinations, PC1 (Ar-F1/Th-F1/WR2) and PC2 (Ar-F1/Th-F4/WR2), were designed and repeatedly screened to distinguish two strains simultaneously, of which two special forward primers Th-F1/Th-F4 were used for the thelytokous strain and one special forward primer Ar-F1 was used for the arrhenotokous strain. In addition, a common reverse primer, WR2, was used for both strains. The PC1 and PC2 PCR assays were effective in distinguishing the two strains at different developmental stages and field colonies. This method provides a reliable, highly sensitive, and cost-effective tool for the rapid identification of the two strains of D. wani.

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A new species of Tamarixia Mercet (Hymenoptera, Eulophidae), a parasitoid of Diaphorina communis Mathur (Hemiptera, Liviidae) in Bhutan

Cited by 5 publications

References 38 publications

Genetic Diversity of Tamarixia radiata Populations and Their Associated Endosymbiont Wolbachia Species from China

Genetic Diversity of Tamarixia radiata Populations and Their Associated Endosymbiont Wolbachia Species from China

Incidence of psyllids and ‘Candidatus Liberibacter asiaticus’ in mandarin orchards at different altitudes in Tsirang, Bhutan

A One-Step Multiplex PCR Method to Rapidly Distinguish Two Strains of Diglyphus wani (Hymenoptera: Eulophidae) Against Agromyzid Leafminers

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