A novel electroconductive interface based on Fe₃O₄ magnetic nanoparticle and cysteamine functionalized AuNPs: Preparation and application as signal amplification element to minoring of antigen‐antibody immunocomplex and biosensing of prostate cancer

Abstract

In this study, a novel electroconductive interface was prepared based on Fe3O4 magnetic nanoparticle and cysteamine functionalized gold nanoparticle. The engineered interface was used as signal amplification substrate in the electrochemical analysis of antibody‐antigen binding. For this purpose, biotinilated‐anti‐prostate‐specific antigen (PSA) antibody was bioconjugated with iron oxide magnetic nanoparticles (Fe3O4) and drop‐casted on the surface of glassy carbon electrode (GCE). Also, secondary antibody (HRP‐Ab2) encapsulated on gold nanoparticles caped by cysteamine was immobilized on the surface of GCE modified electrode. A transmission electron microscopy images shows that a sandwich immunoreaction was done and binding of Ab1 and Ab2 performed successfully. Various parameters of immunoassay, including the loading of magnetic nanoparticles, the amount of gold nanoparticle conjugate, and the immunoreaction time, were optimized. The detection limit of 0.001 μg. L−1 of PSA was obtained under optimum experimental conditions. It is found that such magneto‐bioassay could be readily used for simultaneous parallel detection of multiple proteins by using multiple inorganic metal nanoparticle tracers and are expected to open new opportunities for early stage diagnosis of cancer in near future.

A novel immunosensor for the monitoring of PSA using binding of biotinylated antibody to the prostate specific antigen based on nano-ink modified flexible paper substrate: efficient method for diagnosis of cancer using biosensing technology

“…Prostate cancer is the most significant reason for deaths in men, outside of lung cancer. The clinical examination of cancer proteins or biomarkers is extremely significant in early examination and monitoring of recurrence of disease after treatment [ 3 ]. In addition to during prognosis, biomarkers usually increased a lot of clinical importance because of their extensive spectra in the identification, elimination, early diagnosis and cure of cancer.…”

Gold Nanoparticles Based Optical Biosensors for Cancer Biomarker Proteins: A Review of the Current Practices

“…Secondary antibody (HRP-Ab2) encapsulated on AuNPs covered by cysteamine was fixed on the GCE modified electrode. Under ideal experimental circumstances, a detection limit of 0.001 µg.L −1 of PSA was achieved ( Farshchi et al, 2020 ). In another study, Mahani et al, reported.…”

Enzymatic recognition of hydrogen peroxide (H2O2) in human plasma samples using HRP immobilized on the surface of poly(arginine‐toluidine blue)‐ Fe3O4 nanoparticles modified polydopamine; A novel biosensor

“…MNPs were prepared by the conventional co-precipitation process. 41 on the CV technique. 43,44 According to Figures S7 and S8 (see supporting information), anodic and cathodic peak currents of P(L-Arg/Tb) and PDA-MNPs/L-Arg/Tb vary with scan rate, and by progress of scan rate, the peak separation (ΔE) increases, (ΔE p , is calculated by E pc À E pa ).…”