A novel IL-10-producing innate lymphoid cells (ILC10) in a contact hypersensitivity mouse model

Abstract

The immunoregulatory cytokine Interleukin 10 (IL-10) protein is produced by various cells during the course of inflammatory disorders. Mainly, it downregulates pro-inflammatory cytokines, antigen presentation, and helper T cell activation. In this study, we show that the ratio of IL-10-producing cells was significantly increased in lineage negative (i.e., not T, B, or leukocyte cell lineages) cells than in lineage positive cells in lymphoid and peripheral tissues. We further observed that IL-10-producing innate lymphoid cells (ILCs), here called firstly ILC10, were increased in number in oxazolone-induced contact hypersensitivity (CHS) mice. In detail, IL-10-producing lineage negative cells were elevated in the axillary, inguinal lymph node, and ear tissues of CHS mice. Notably, the cells expressed classical ILC marker proteins such as CD45, CD127, and Sca-1. Altogether, our findings suggest for the first time that ILC10s are present in various physiological settings and could be involved in numerous immune responses as regulatory cells. [BMB Reports 2016; 49(5): 293-296]

Dissecting human ILC heterogeneity: more than just three subsets

“…Recently, a new ILC subset [regulatory subpopulation (ILC reg ) or ILC10] has been identified in mice. 49,50 Transcriptome analysis revealed that these cells constitute a unique subset of ILC that produces regulatory cytokine, but did not express the transcription factor forkhead box protein 3 (FoxP3). Murine ILC reg contribute to resolving intestinal inflammation by producing IL-10 and transforming growth factor (TGF)-b.…”

New insights into the function, development, and plasticity of type 2 innate lymphoid cells

“…Recently, independent studies in mice demonstrated the capability of ILCs to produce IL‐10. Kim et al showed increased numbers of IL‐10‐producing non T‐, B‐, or leukocyte cell lineage in the model of oxazolone‐induced contact hypersensitivity. These IL‐10‐producing CD45 + CD127 + Sca‐1 + cells were elevated in the axillary, inguinal lymph node, and ear tissue.…”

The expanding family of noncanonical regulatory cell subsets

“…Kim et al. first proved that IL‐10‐producing innate lymphoid cells are present in various physiological settings and could be involved in numerous immune responses as regulatory cells . It was Wang et al.…”

“…FOXP3 + Tregs play critical roles in maintaining immune tolerance, an analogous FOXP3-expressing ILC subset has not been defined so far. The lack of adequate characterization has raised the question as to whether a regulatory ILC subset exists.Kim et al first proved that IL-10-producing innate lymphoid cells are present in various physiological settings and could be involved in numerous immune responses as regulatory cells 115. It was Wang et alwho finally identified ILCreg as equivalent to conventional Tregs, as shown by the expression of CD45 and CD127 and the production of suppressive IL-10 113.…”