A possible role for membrane depolarization in epithelial wound healing

Chifflet, Silvia; Hernández, Julio A.; Grasso, Silvina

doi:10.1152/ajpcell.00259.2004

Cited by 83 publications

(118 citation statements)

References 63 publications

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“…Previous studies have shown that VSMC migration, from the media into the intima, plays a significant role following arterial injury, and blocking VSMC migration can reduce the neointimal lesion size (Rutherford et al, 1997;Slepian et al, 1998). A role for DEG/ ENaC/ASIC proteins in cellular migration has been suggested in recent reports (Chifflet et al, 2005;Grifoni et al, 2006;Vila-Carriles et al, 2006). Our previous study showed that DEG/ ENaC/ASIC pharmacological inhibition with amiloride and its analog benzamil inhibit VSMC migration.…”

Section: Discussionmentioning

confidence: 91%

“…Recently, others and we have shown members of the Degenerin/Epithelial Sodium (Na + ) Channel/Acid Sensing-Ion Channel (DEG/ENaC/ASIC) protein family, contribute to cellular migration in glial, epithelial and VSM cells (Chifflet et al, 2005;Grifoni et al, 2006;VilaCarriles et al, 2006). Members of this family share a similar structure, two membrane spanning domains separated by a large extracellular domain and intracellular N-and C-termini.…”

Section: Introductionmentioning

confidence: 99%

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ASIC proteins regulate smooth muscle cell migration

Grifoni

Jernigan

Hamilton

et al. 2008

Microvascular Research

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The purpose of the present study was to investigate Acid Sensing Ion Channel (ASIC) protein expression and importance in cellular migration. We recently demonstrated Epithelial Na + Channel (ENaC) proteins are required for vascular smooth muscle cell (VSMC) migration, however the role of the closely related ASIC proteins has not been addressed. We used RT-PCR and immunolabeling to determine expression of ASIC1, ASIC2, ASIC3 and ASIC4 in A10 cells. We used small interference RNA to silence individual ASIC expression and determine the importance of ASIC proteins in wound healing and chemotaxis (PDGF-bb) initiated migration. We found ASIC1, ASIC2, and ASIC3, but not ASIC4, expression in A10 cells. ASIC1, ASIC2, and ASIC3 siRNA molecules significantly suppressed expression of their respective proteins compared to non-targeting siRNA (RISC) transfected controls by 63%, 44%, and 55%, respectively. Wound healing was inhibited by 10, 20 and 26% compared to RISC controls following suppression of ASIC1, ASIC2, and ASIC3, respectively. Chemotactic migration was inhibited by 30% and 45%, respectively following suppression of ASIC1 and ASIC3. ASIC2 suppression produced a small, but significant, increase in chemotactic migration (4%). Our data indicate ASIC expression is required for normal migration and may suggest a novel role for ASIC proteins in cellular migration.

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Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

ASIC proteins regulate smooth muscle cell migration

Grifoni

Jernigan

Hamilton

et al. 2008

Microvascular Research

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“…However, PcTX-1 prevented a regulatory volume increase in glioma cells following a hyperosmotic challenge (19). It has previously been suggested that in addition ENaC plays an important role in wound healing (33,34) and proliferation (35). Both ASIC and ENaC channels contribute to migration of vascular smooth muscle cells (36,37), although in neither case are the underlying mechanisms well understood.…”

Section: Discussionmentioning

confidence: 99%

Glioma-specific Cation Conductance Regulates Migration and Cell Cycle Progression

Rooj

McNicholas

Bartoszewski

et al. 2012

Journal of Biological Chemistry

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Background: Cation transport contributes to migration and proliferation of tumor cells. Results: Sodium current block decreased ERK phosphorylation and increased expression of cell cycle inhibitors. Conclusion: Activity of an ENaC/ASIC cation channel is required to maintain the glioma cell phenotype. Significance: Activity of a membrane cation channel influences signaling pathways to effect changes in migration and proliferation of glioma cells.

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“…Current literature reports that different mammalian cell types possess different degrees of well-defined V mem states. Hence, the V mem state might be a cellspecific marker in many cases, such as in proliferative (Binggeli and Weinstein, 1986;Marino et al, 1994) V mem acts in olfactory nerve regeneration of the tiger salamander (Masukawa et al, 1985), in development (Hotary and Robinson, 1994) and leftright asymmetry (Adams et al, 2006) of Xenopus embryos, in Xenopus tail regeneration (Adams et al, 2007), in epithelial wound healing of bovine cornea (Chifflet et al, 2005), in autocrine activation of two-cell mouse embryos (Li et al, 2007), K þ channel expression and proliferation of oligodendrocyte progenitor cells (Knutson et al, 1997), and adherens junctions and reorganization of the actin cytoskeleton in bovine corneal endothelial cells (Nin et al, 2009). Consistent with studies in Xenopus (Adams et al, 2007), we found that V mem shows a significant degree of depolarization, especially at 6 hpa (five-fold) in the regeneration bud vs. uncut tail in axolotl embryos.…”

Section: Memmentioning

confidence: 99%

Ion imaging during axolotl tail regeneration in vivo

Özkucur

Epperlein

Funk

2010

Developmental Dynamics

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Several studies have reported that endogenous ion currents are involved in a wide range of biological processes from single cell and tissue behavior to regeneration. Various methods are used to assess intracellular and local ion dynamics in biological systems, e.g., patch clamping and vibrating probes. Here, we introduce an approach to detect ion kinetics in vivo using a noninvasive method that can electrophysiologically characterize an entire experimental tissue region or organism. Ion-specific vital dyes have been successfully used for live imaging of intracellular ion dynamics in vitro. Here, we demonstrate that cellular pH, cell membrane potential, calcium, sodium and potassium can be monitored in vivo during tail regeneration in the axolotl (Ambystoma mexicanum) using ion-specific vital dyes. Thus, we suggest that ion-specific vital dyes can be a powerful tool to obtain electrophysiological data during crucial biological events in vivo.

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A possible role for membrane depolarization in epithelial wound healing

Abstract: Chifflet, Silvia, Julio A. Herná ndez, and Silvina Grasso. A possible role for membrane depolarization in epithelial wound healing.

Cited by 83 publications

References 63 publications

ASIC proteins regulate smooth muscle cell migration

ASIC proteins regulate smooth muscle cell migration

Glioma-specific Cation Conductance Regulates Migration and Cell Cycle Progression

Ion imaging during axolotl tail regeneration in vivo

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