A quantitative study of changes in old age of 5-hydroxytryptamine-like immunoreactivity in perivascular nerves of the rabbit

Gale, Jeremy D.; Alberts, Jared; Cowen, T.

doi:10.1016/0165-1838(89)90007-6

Cited by 11 publications

(2 citation statements)

References 18 publications

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“…long pass filter of approximately 560 nm was used to reduce autofluorescence. The program for measurement of immunoreactivity was derived from one used in previous studies [18][19][20]. Ten digitised images of the field to be measured were summed and then averaged to reduce background noise.…”

Section: Immunohistochemical Measurements Using Computerised Image-anmentioning

confidence: 99%

Immunohistochemical measurements of nerves and neuropeptides in diabetic skin: relationship to tests of neurological function

et al. 1992

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Image-analysis was used to measure nerves immunoreactive to the general neuronal marker protein gene product 9.5 (PGP 9.5-IR) and the neuropeptides calcitonin gene-related peptide and vasoactive intestinal polypeptide in standardised leg skin biopsies of three age-matched groups of young subjects: non-diabetic (n = 14), diabetic patients with normal small fibre function ("non-neuropathic", (n = 11) and diabetic patients with abnormal small fibre function ("neuropathic", n = 11). Depletion of nerves and neuropeptides was most marked in the epidermis, where calcitonin gene-related peptide-immunoreactivity was more frequently absent than PGP 9.5-IR in diabetic patients. Epidermal PGP 9.5-IR nerve area and counts were reduced in neuropathic compared with normal subjects (p less than 0.001), as were epidermal calcitonin gene-related peptide nerve counts (p = 0.003). Sweat gland PGP 9.5 and vasoactive intestinal polypeptide, which may be involved in sweat production, showed no diminution in diabetic patients (area: p = 0.160, p = 0.372 by ANOVA). Two diabetic patients showed elevated sweat gland PGP 9.5-IR and three had increased sweat gland vasoactive intestinal polypeptide; this may represent nerve proliferation. In local sweat tests, acetylcholine-stimulated sweat output was associated with increased immunoreactivity, while the sympathetic skin response showed inverse correlations with immunoreactivity. There were no consistent changes with other commonly-used neurophysiological tests. HbA1 correlated negatively with immunohistochemical measurements. Neuropeptide changes were seen in the absence of macro- and microvascular disease, and epidermal nerve depletion occurred in patients with normal thermal thresholds and cardiac autonomic function.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Immunohistochemical Measurements Using Computerised Image-anmentioning

confidence: 99%

Immunohistochemical measurements of nerves and neuropeptides in diabetic skin: relationship to tests of neurological function

et al. 1992

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“…nerve fibers has been demonstrated in the perivascular nerves of different vascular beds (Costa et al, 1982;Dahlstrom and Ahlman, 1983;Gale and Cowen, 1988;Gale et al, 1989) where they are probably colocalized with noradrenaline in sympathetic nerve fibers (Gale et al, 1989;Cowen et al, 1986Cowen et al, , 1987Owman et al, 1990). Moreover, the existence of a 5-HT-ergic innervation of the gastrointestinal tract has been described (Gershon et al, 1990).…”

Section: The Occurrence Of 5-ht-like Immunoreactive (Ir)mentioning

confidence: 92%

Localization of 5‐hydroxytryptamine‐like immunoreactive cells and nerve fibers in the rat female reproductive system

Amenta

Rícci

et al. 1992

Anat. Rec.

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The presence of 5-hydroxytryptamine (5-HT)-like immunoreactivity (IR) was studied in the rat female reproductive system using polyclonal antibodies directed against 5-HT. Moreover, 5-HT levels in the ovary, oviduct, uterus, and cervix were measured by high-pressure liquid chromatography with electrochemical detection. The highest 5-HT concentrations were found in the oviduct, followed in descending order by the cervix, the ovary, and the uterus. Most 5-HT-like IR was observed in the cytoplasm of mast cells. These cells were found in the connective tissue around the fimbria, in the oviduct, in the uterus, and in the ovary. Mast cells are clustered in the proximity of the parenchymal blood vessels. Moreover, a few 5-HT-like nerve fibers were found distributed mainly perivascularily in the uterine cervix and in the uterine horns as well as in the oviduct. IR nerve fibers were rarely seen within the ovary. The present data provide direct evidence that 5-HT in the female reproductive system not only is associated with mast cells but is located in nerve fibre-like structures as well. The functional significance of this probable 5-HT-ergic innervation of the female reproductive tract discovered in the present study should be clarified in future investigations.

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Image analysis quantification of peptide‐immunoreactive nerves in the skin of patients with Raynaud's phenomenon and systemic sclerosis

Terenghi

Bunker

Liu

et al. 1991

The Journal of Pathology

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Image analysis quantification was used to assess the results of immunocytochemistry for a neuronal marker and neuropeptides in digital skin biopsies from Raynaud's phenomenon (RP) and systemic sclerosis (SS) patients, to verify the possibility of a selective quantitative abnormality of immunoreactive nerves. The field area of specific immunostaining and nerve counts were evaluated on coded specimens, and the data compared by statistical analysis. Nerves immunoreactive for protein gene product 9.5 (PGP), a marker for neuronal elements, were decreased significantly in epidermal and subepidermal layers of digital skin in RP patients (P less than 0.0001). This change was paralleled by a decrease of calcitonin gene-related peptide (CGRP) immunoreactive nerves in the epidermis and around capillaries in the dermal papillae (P = 0.005). In the skin of RP patients, these changes were readily demonstrated by image analysis, although they were not always apparent on visual screening. In digital skin of SS patients, there was a generalized and very significant decrease of PGP, CGRP, and VIP immunoreactivities in all areas (P less than 0.0001). These results demonstrate that neuropeptide-containing nerves are involved in the digital pathology of RP and SS, and that image analysis quantification is an accurate and sensitive method for assessing morphological changes in pathological samples.

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A quantitative study of changes in old age of 5-hydroxytryptamine-like immunoreactivity in perivascular nerves of the rabbit

Cited by 11 publications

References 18 publications

Immunohistochemical measurements of nerves and neuropeptides in diabetic skin: relationship to tests of neurological function

Immunohistochemical measurements of nerves and neuropeptides in diabetic skin: relationship to tests of neurological function

Localization of 5‐hydroxytryptamine‐like immunoreactive cells and nerve fibers in the rat female reproductive system

Image analysis quantification of peptide‐immunoreactive nerves in the skin of patients with Raynaud's phenomenon and systemic sclerosis

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