A simple and rapid biosensor for ochratoxin A based on a structure-switching signaling aptamer

Chen, Junhua; Fang, Zhiyuan; Liu, Jie; Zeng, Lingwen

doi:10.1016/j.foodcont.2011.11.039

Cited by 168 publications

(126 citation statements)

References 39 publications

Supporting

Mentioning

120

Contrasting

Order By: Relevance

“…Although HPLC, TLC, and more recently, ELISA are commonly used for AFM1 analysis, most of these methods involve expensive instruments and time-consuming [32]. Aptamers offer another opportunity as a simple, safe, rapid, and robust technique and this method has been testing in many other systems with success [13,33,34]. In this study, those aptamers that were attached to ferrous oxide nanoparticles were used for wiping off the toxic effect of AFM 1 .…”

Section: Discussionmentioning

confidence: 99%

“…Aptamers synthesized simply in large amount by the chemical method and are resistant enough to biological temperature and can operate actions [1,13]. The attachment of aptamer to nanomagnetic particles improves its synthetic qualities, increases the attachment of target molecules, and eases the detachment of aptamer complex from target molecule [1,14].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Aflatoxin M1 detoxification from infected milk using Fe3O4 nanoparticles attached to specific aptamer

et al. 2017

View full text Add to dashboard Cite

Aflatoxins are a kind of mycotoxins that are mostly produced by a group of molds such as Aspergillus flavus and Aspergillus parasiticus. The studies on different parts of Iran showed that AFM 1 infection of milk is higher than European Union's standard. In this study, the specific aptamer with the carboxylic group at the end of 5′ and Fe 3 O 4 nanoparticles with amine groups was synthesized. Morphological and structural qualities of Fe 3 O 4 were determined by the Fourier-transform infrared spectrograph (FTIR), dynamic light scattering, scanning electron microscope, and X-ray diffraction devices. The specificity of the aptamer to AFM 1 was investigated in adjacent of AFM 1 and aflatoxin B 1 . Then, aptamers were attached to nanoparticles to improve synthetic qualities and to ease of its detachment. The attachment was approved by FTIR method. The complex (Fe 3 O 4 -APT) was then added to infected milk and after the proper time was detached from the milk using a magnet. The remained amount of AFM 1 was attained in milk using high-performance liquid chromatography. Our result showed that this method for aflatoxin detoxification is much more effective than conventional methods based on recognition of AFM 1 and their concentration in infected milk. This method is more applicable, faster, and cheaper highlighting its application in milk factories. Graphical abstractKeywords Aflatoxin · Aptamer · Fe 3 O 4 nanoparticle · Detoxification · Mycotoxin

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Aflatoxin M1 detoxification from infected milk using Fe3O4 nanoparticles attached to specific aptamer

et al. 2017

View full text Add to dashboard Cite

show abstract

“…OTA is nephrotoxic and carcinogenic and poses a serious threat to the health of both humans and animals [74]. The International Agency for Research on Cancer (IARC) has considered OTA as a potential carcinogen.…”

Section: Biotoxinsmentioning

confidence: 99%

“…The International Agency for Research on Cancer (IARC) has considered OTA as a potential carcinogen. International and governmental agencies in many countries regulate the OTA content in a number of basic agro-products and foods [74]. Recently, OTA has been detected in various foods, agroproducts, and feed sources.…”

Section: Biotoxinsmentioning

confidence: 99%

“…Recently, OTA has been detected in various foods, agroproducts, and feed sources. Several methods have been developed for the analysis of OTA in a variety of food commodities including cereals, cocoa, coffee, wine, beer, and dried fruits [74]. The European Commission (EC) required that the maximum level of OTA in raw cereal grains, cereal-derived products, and soluble coffee are at 5, 3, and 10 μg/kg, respectively (Commission Regulation No.…”

Section: Biotoxinsmentioning

confidence: 99%

See 1 more Smart Citation

Aptamer-Based Technology for Food Analysis

Liu

Zhang

2014

Appl Biochem Biotechnol

View full text Add to dashboard Cite

Aptamers are short and functional single-stranded oligonucleotide sequences selected from systematic evolution of ligands by exponential enrichment (SELEX) process, which have the capacity to recognize various classes of target molecules with high affinity and specificity. Various analytical aptamers acquired by SELEX are widely used in many research fields, such as medicine, biology, and chemistry. However, the application of this innovative and emerging technology to food safety is just in infant stage. Food safety plays a very important role in our daily lives because varieties of poisonous and harmful substances in food affect human health. Aptamer technique is promising, which can overcome many disadvantages of existing detection methods in food safety, such as long detection time, low sensitivity, difficult, and expensive antibody preparation. This review provides an overview of various aptamer screening technologies and summarizes the recent applications of aptamers in food safety, and future prospects are also discussed.

show abstract

Review of Sample Treatments and the State‐of‐the‐art of Analytical Techniques for Mycotoxins in Food

Arroyo‐Manzanares

Huertas-Pérez

Garcı́a-Campaña

et al. 2017

Analysis of Food Toxins and Toxicants

View full text Add to dashboard Cite

A simple and rapid biosensor for ochratoxin A based on a structure-switching signaling aptamer

Cited by 168 publications

References 39 publications

Aflatoxin M1 detoxification from infected milk using Fe3O4 nanoparticles attached to specific aptamer

Aflatoxin M1 detoxification from infected milk using Fe3O4 nanoparticles attached to specific aptamer

Aptamer-Based Technology for Food Analysis

Review of Sample Treatments and the State‐of‐the‐art of Analytical Techniques for Mycotoxins in Food

Contact Info

Product

Resources

About