A study of biochemical polymorphism in carp (Cyprinus Carpio): Detect new alleles in transferrin

Abstract

This study was carried out at the college of agriculture and marine science centre, Basrah university. The present study was conducted to investigate the existence of polymorphism at transferrin (Tf) locus in the Carp (Cyprinus carpio). A polyacrylamide gel electrophoresis (PAGE) under alkaline condition method was used to distinguish Carp Tf alleles. Use the gel documentation program in this study. Analysis of 116 animals revealed that all animals were polymorphic, showing many genotypes. There was very clear biodiversity in the Tf gene. Seven Tf genotypes consisting of 4 homozygote types (CC, DD, FF and GG) and two heterozygote types (CD, DG and FG) were detected. These fractions are controlled by co-dominant autosomal genes according to the Mendelian laws of inheritance. The highest gene frequencies were calculated 0.50 for Tf D, 0.26 for Tf F and 0.12 for C and G. thus, carp (Cyprinus carpio) assemblages consistently tended to be more predominant to D allele. Differences between expected number and observed number for transferrin genotypes were no significant. This is useful in genetic improvement process through the selection. As far as we know, this is the first large-scale analysis on the genetic polymorphism in carp (Cyprinus carpio). Polyacrylamide electrophoresis, the technique employed in this study, allows rapid and efficient screening for the presence of polymorphism in Tf.

Transferrin in fishes: A review article

“…Two distinct Atlantic cod stocks at Faroe Plateau and Faroe Bank were reported based on the frequencies of five cod transferrin alleles [73]. Jaayid et al investigated the existence of polymorphisms at transferrin locus in carp (Cyprinus carpio) and concluded that there existed seven transferrin genotypes consisting of 4 homozygote types (CC, DD, FF and GG) and two heterozygote types (CD, DG and FG) [74]. A relationship between sperm motility parameters and transferrin polymorphism of carp seminal plasma, which may affect sperm competitive ability, has been reported by Wojtczak et al [75].…”

Phenotypic connection of the main body parts of rabbits and layers

“…Transferrin, one of class I genetic markers, is the most heterogeneous polymorphic blood protein in Carp (Valenta et al, 1976and Csizmadia et al, 1995), in goose (Valenta and Stratil, 1978), in chicken (Vyshinsky and Muravjev, 1970 and sheep (Jaayid et al, 2011), a total of 7 co-dominant alleles have been T.A. Jaayid et al 266 found in its locus.…”