Due to the world’s growing population, the size of areas intended for food production in many countries of the world can only be achieved through severe environmental damage and deforestation, which has many other detrimental consequences in addition to accelerating global warming. By replacing the bio-content of fuels with other alternative fuels, land that is used for energy crops can also be used to grow food, thus mitigating the damaging effects of deforestation. Waste-based tire pyrolysis oil (TPO) can be a promising solution to replace the bio-proportion of diesel fuel. Since it is made from waste tires, it is also an optimal solution for recycling waste. This research shows the effect of different low-volume-percent tire pyrolyzed oil blended with diesel on the performance, fuel consumption, and emissions on a Mitsubishi S4S-DT industrial diesel engine. Four different premixed ratios of TPO were investigated (2.5%, 5%, 7.5% and 10%) as well as pyrolysis oil and 100% diesel oil; however, the following studies will only include the data from the pure diesel and the 10% TPO measurements. The experimental investigations were in an AVL electric dynamometer, the soot measurements were in an AVL (Anstalt für Verbrennungskraftmaschinen List) Micro soot sensor (MSS), and the emission measurements were in a AVL Furier-transform infrared spectroscopy (FTIR) taken. The scope of research was to investigate the effect of low volume percentage TPO on performance and emissions on a light-duty diesel engine.