A Study of Proliferative Activity of Herbs Eriocaulon Cinereum R.Br on Cervical Cancer Cells (HeLa) with MTT Assay Method

Nugraha, Arde Toga; Ramadhan, Viren; Pandapotan, Herianto; Romadhonsyah, Fitra

doi:10.18178/ijpmbs.6.2.73-76

Cited by 4 publications

(6 citation statements)

References 9 publications

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“…E. cinereum has not been intensively studied for their property as anticancer compared to other genus of Eriocaulon [9][10][11][12]. The finding of the antiproliferative activity of the ethanol extract of E. cinereum in HeLa cells from our preliminary study [13] led to our further investigation to evaluate the cytotoxic activity of E. cinereum against HeLa cell lines.…”

Section: Discussionmentioning

confidence: 87%

“…This plant has been used by local people to prevent and treat uncontrolled cells growth. The result showed the inhibition of HeLa cells growth with the half maximal inhibitory concentration (IC 50 ) value of 428 µg/mL on ethanol extract [13]. Based on this result, further investigation of E. cinereum was carried out as a source for new anticancer therapeutics.…”

Section: Introductionmentioning

confidence: 94%

“…Nugraha et al [13] conducted a preliminary study to screening the Eriocaulon cinereum R.Br. from Bangka Belitung Island, Indonesia, for anticancer.…”

Section: Introductionmentioning

confidence: 99%

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Cytotoxic Activity Evaluation of Eriocaulon Cinereum R.BR. On Hela and Vero Cell Lines

et al. 2019

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Objective: This study aimed to evaluate the cytotoxic activity of the extracts and fractions of Eriocaulon cinereum against HeLa and Vero cell lines,which represent cervical cancer and normal cells, respectively. In addition, a phytochemical screening was carried out to determine the chemicalconstituents in the extracts and the active fractions.Materials and Methods: The extracts of E. cinereum were obtained by ultrasound-assisted extraction method using n-hexane, ethyl acetate, andmethanol, successively. The active extract was fractionated using vacuum liquid chromatography with dichloromethane followed by ethyl acetate. Thecytotoxic activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay method and was measured using microplatereader at the wavelength 595 nm. The data were analyzed with PROBIT from SPSS 16 for Windows®. In addition, phytochemical screening wasperformed using standard procedures.Results: The cytotoxic evaluation of the extracts of E. cinereum showed that the ethyl acetate extract was the most active extract against HeLa cell linewith the half maximal inhibitory concentration (IC50) value of 580.07 μg/ml. The dichloromethane and ethyl acetate fractions from the active extractof E. cinereum exhibited cytotoxic activity against HeLa cell with the IC50 values of 466.61 μg/ml and 267.34 μg/ml, respectively. In addition, the ethylacetate fraction showed a low cytotoxic effect against Vero cell line. The phytochemical screening of the ethyl acetate fraction indicated the presenceof terpenoids and alkaloids.Conclusion: This finding revealed the anticancer potential of E. cinereum and warranted further investigation for the discovery of new anticanceragents from natural resources for cervical cancer.

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Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 94%

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Cytotoxic Activity Evaluation of Eriocaulon Cinereum R.BR. On Hela and Vero Cell Lines

et al. 2019

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“…Penelitian pendahuluan menunjukkan bahwa ekstrak etanol rumput gong mampu menghambat pertumbuhan sel HeLa yang merupakan sel kanker serviks dengan IC 50 sebesar 427,79 µg/ml 10 . Lebih lanjut, penelitian terhadap ekstrak etil asetat dan metanol rumput gong memperlihatkan adanya aktivitas sitotoksik terhadap sel HeLa dengan IC 50 masingmasing sebesar 580,07 μg/ml 11 dan 626,41 μg/ml 12…”

Section: Pendahuluanunclassified

Evaluasi Potensi Fraksi Rumput Gong (Eriocaulon cinereum R. Br) sebagai Antikanker Serviks terhadap sel HeLa

et al. 2020

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Rumput gong (Eriocaulon cinereum R. Br.) merupakan tumbuhan yang secara tradisional telah dipergunakan masyarakat Bangka Belitung untuk mencegah pertumbuhan kanker. Penelitian pendahuluan memperlihatkan bahwa ekstrak etil asetat dan metanol rumput gong memiliki aktivitas sitotoksik terhadap sel HeLa dengan IC50 masing-masing sebesar 580,07 μg/ml dan 626,41 μg/ml. Hal ini menunjukkan bahwa rumput gong berpotensi untuk dikembangkan sebagai bahan baku obat antikanker serviks. Dalam upaya pengembangan tersebut perlu diketahui senyawa yang bertanggung jawab terhadap aktivitas sitotoksik terhadap sel HeLa. Sebagai langkah awal untuk memperoleh senyawa aktif antikanker serviks dari rumput gong maka dilakukan fraksinasi terhadap ekstrak serta evaluasi aktivitas sitotoksiknya terhadap sel HeLa. Penelitian ini bertujuan untuk mengkaji aktivitas sitotoksik fraksi dari ekstrak etil asetat dan metanol rumput gong terhadap sel HeLa. Ekstrak etil asetat dan metanol rumput gong diperoleh melalui maserasi bertingkat dengan bantuan sonikasi. Selanjutnya ekstrak etil asetat rumput gong difraksinasi menggunakan kromatografi cair vakum dengan fase gerak diklorometana dan etil asetat, sedangkan ekstrak metanol rumput gong difraksinasi menggunakan fraksinasi cair-cair secara bertingkat dengan diklorometana, kloroform dan air. Aktivitas sitotoksik dari masing-masing fraksi dievaluasi menggunakan metode MTT assay dan diukur dengan microplate reader pada panjang gelombang 595 nm. Hasil uji aktivitas sitotoksik dianalisa secara statistik menggunakan analisis PROBIT dengan SPSS 16 for Windows®. Fraksi diklorometana dan etil asetat dari ekstrak etil asetat rumput gong menunjukkan aktivitas sitotoksik terhadap sel HeLa dengan IC50 masing-masing sebesar 466,61 μg/ml dan 267,34 μg/ml. Nilai IC50 fraksi-fraksi yang diperoleh dari ekstrak metanol rumput gong adalah 235,65 μg/ml (fraksi diklorometana) dan 2325,30 μg/ml (fraksi air), sedangkan fraksi kloroform tidak memperlihatkan adanya aktivitas sitotoksik. Fraksi etil asetat dari ekstrak etil asetat rumput gong dan fraksi diklorometana dari ekstrak metanol memiliki aktivitas sitotoksik yang paling poten terhadap sel HeLa. Dengan demikian fraksi-fraksi tersebut berpotensi untuk dilakukan pengkajian lebih lanjut sebagai antikanker serviks.

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“…Treatment of HPV16 or HPV18 positive human cervical cancer with dietary Fls, including naringenin, naringin, and hesperetin, can lead to concentration-and time-dependent inhibition of cell proliferation through the improvement of apoptosis and the induction of cell cycle arrest [50]. Nugraha et al (2017) showed that Fls derived from Eriocaulon cinereum extraction suppressed the growth of HeLa cells with good IC50 value [51]. While hesperetin exhibits apoptotic action against SiHa cervical cancer cells [52], the anticancer activities of 26 flavanone derivatives on colorectal cancer cells (HCT116) were reported to block G1 cell cycle progression and inhibit the clonogenicity of cells [53].…”

Section: Flavonoidsmentioning

confidence: 99%

Investigation of antitumor properties of semi-synthetic flavonoid derivatives on gynecological cancer cell lines

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A Study of Proliferative Activity of Herbs Eriocaulon Cinereum R.Br on Cervical Cancer Cells (HeLa) with MTT Assay Method

Cited by 4 publications

References 9 publications

Cytotoxic Activity Evaluation of Eriocaulon Cinereum R.BR. On Hela and Vero Cell Lines

Cytotoxic Activity Evaluation of Eriocaulon Cinereum R.BR. On Hela and Vero Cell Lines

Evaluasi Potensi Fraksi Rumput Gong (Eriocaulon cinereum R. Br) sebagai Antikanker Serviks terhadap sel HeLa

Investigation of antitumor properties of semi-synthetic flavonoid derivatives on gynecological cancer cell lines

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