A study on microbiology culture of acute appendicectomy specimen and its correlation with wound infection

Abstract

Background: Appendicitis is a common condition which frequently requires emergency surgery and has postoperative wound infection has been reported in few cases which can be influenced by many factors, the most important being surgical skill and technique and the criteria used to define the infection. Bacteria play an important role in appendicitis and the local application of antibiotics or antiseptics can reduce the incidence of wound infection. In this study, we analyse the microbiology culture of acute appendicectomy specimen and its correlation with wound infection.Methods: This is a randomised control test study which was carried out 56 patients with appendicitis. The swab was taken from appendix lumen after appendicectomy and wound infection was sent to laboratory to carry out histology findings and infective organisms respectively. All patients were followed postoperatively for wound infection.Results: Bacteria was isolated from 60% swabs taken from appendix lumen in which gram-negative bacilli isolated as commonest bacteria among which perforated appendicitis shows 66.7% isolation of bacteria. Wound infection among patients underwent appendicectomy was 21%. Gram-negative bacilli were isolated from almost all swabs (100%) taken from wound infection.Conclusions: From this study and results, it shows gram negative bacilli was the commonest organisms isolated from the swabs taken from both appendix lumen and wound infection.

Surgical Site Infection;

“…Age (mean ± SD) in control and cases was observed as 25.5 ± 8.5 and 24.5 ± 9.5 years respectively (P=0.76). Table-II These findings are supported by previous studies 11,12 as they have reported similar young age subjects. In present study, the male and female were noted as 43 (%) and 17 (%) & 45 (%) and 15 (%) in control and cases respectively (P >0.05).…”

“…In present study, the male and female were noted as 43 (%) and 17 (%) & 45 (%) and 15 (%) in control and cases respectively (P >0.05). Male dominancy was noted, this is supported by previous studies [11][12][13] as they have reported predominantly male subjects. Blood glucose was performed to exclude an incidental case of diabetes mellitus as this delays the wound healing and predisposes to the surgical site wound infections.…”

“…Food and Drug Administration (FDA) approves its use as bactericidal agent for acute superficial wounds infections. [9][10][11] Hence, PI is ideal for contaminated surgical procedures such as the appendectomy 1 for prevention of surgical site infection (SSI). The surgical site infections increase hospital stay, increase morbidity and economical expense, looses patients confidence that discourages the surgeons.…”

“…Post operative morbidity, hospital stay and expenses are major problems for patients. 11,12 If we see appendectomy from patient point of view, it is most commonly surgical procedure performed for the youngsters and increases morbidity by surgical site infection increases the economical burden and decreases the earning of poor population. Hence the surgical site infection of post appendectomy is of social concern also.…”

Frequency of wound infection in children undergoing appendectomy for acute versus perforated appendicitis.

“…In patients with severe dehydration and generalized peritonitis personalize electrolytes should be corrected accordingly. 6 Due to the higher incidence of appendicitis and most frequently performed appendectomy procedure surgical treatment of appendix. This study was conducted to find out the wound infection in the perforated appendicitis and acute appendicitis.…”