A survey of Australian haematology reference intervals

Sinclair, Leanne; Hall, Sara; Badrick, Tony

doi:10.1097/pat.0000000000000148

Cited by 10 publications

(6 citation statements)

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“…Defining internal laboratory RIs is a demanding procedure in terms of both time and resources. Thus, many routine laboratories preferably use externally derived RIs, although such approach might be associated with errors and misinterpretation of the obtained laboratory results (4,11,12). Recommendations of CLSI and IFCC strongly support development of internal RIs and more recently, an easier and less consumable indirect method was proposed (7).…”

Section: Discussionmentioning

confidence: 99%

“…To the best of our knowledge, a comprehensive survey of procedures that are used to define RIs in laboratory haematology has not been performed in Serbia. Nonetheless, a similar analysis conducted in Australia could provide a general insight into the status of RIs for haematological parameters (4). Namely, the authors reported that up to 77% of involved laboratories use RIs adopted from other laboratories, textbooks or scientific papers, instead of determination of their own RIs.…”

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confidence: 96%

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Indirect reference intervals for haematological parameters in capillary blood of pre-school children

Balog¹,

Dukai²,

Zeljković

et al. 2021

Biochem. med. (Online)

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Introduction: Indirect estimation of reference intervals (RIs) is straightforward and inexpensive procedure for determination of intra-laboratory RIs. We applied the indirect approach to assess RIs for haematological parameters in capillary blood of pre-school children, using results stored in our laboratory database. Materials and methods: We extracted data from laboratory information system, for the results obtained by automatic haematology analyser in capillary blood of 154 boys and 146 girls during pre-school medical examination. Data distribution was tested, and logarithmic transformation was applied if needed. Reference intervals were calculated by the nonparametric percentile method. Results: Reference intervals were calculated for: RBC count (4.2-5.4 x1012/L), haemoglobin (114-146 g/L), MCH (25.0-29.4 pg), MCHC (321-368 g/L), RDW-SD (36.1-43.5 fL), WBC count (4.5-12.3 x109/L), neutrophils count (1.7-6.9 x109/L) and percentage (29.0-69.0%), lymphocytes count (1.6-4.4 x109/L) and percentage (21.9-60.7%), PLT (165-459 x109/L), MPV (8.1-11.4 fL) and PDW (9.2-14.4%). Gender specific RIs were calculated for monocytes count (male (M): 0.2-1.6 x109/L; female (F): 0.1-1.4 x109/L) and percentage (M: 2.5-18.3%; F: 1.8-16.7%), haematocrit (M: 0.34-0.42 L/L; F: 0.34-0.43 L/L), MCV (M: 73.4-84.6 fL; F: 75.5-84.2 fL) and RDW (M: 12.1-14.3%; F: 11.7-13.9%), due to observed gender differences in these parameters (P = 0.031, 0.028, 0.020, 0.012 and 0.001; respectively). Estimated RIs markedly varied from the literature based RIs that are used in the laboratory. Conclusions: Indirect method employed in this study enables straightforward assessment of RIs in pre-school children. Herein derived RIs differed from the literature-based ones, indicating the need for intra-laboratory determination of RIs for specific populations and sample types.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 96%

Indirect reference intervals for haematological parameters in capillary blood of pre-school children

Balog¹,

Dukai²,

Zeljković

et al. 2021

Biochem. med. (Online)

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“…However, despite these facts and requirements, attempts to establish specific RIs for haematology parameters are still uncommon and are applied to insufficient sample sizes. There have been a limited number of attempts ( 6 , 9 , 10 ) to conduct appropriate multicentre studies to achieve this goal, because with the exception of the concentration of haemoglobin, there are no standard reference materials; native samples must be measured fresh and cannot be measured or re-analysed after storage ( 9 ). …”

Section: Introductionmentioning

confidence: 99%

A nationwide multicentre study in Turkey for establishing reference intervals of haematological parameters with novel use of a panel of whole blood

et al. 2017

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IntroductionA nationwide multicentre study was conducted to establish well-defined reference intervals (RIs) of haematological parameters for the Turkish population in consideration of sources of variation in reference values (RVs).Materials and methodsK2-EDTA whole blood samples (total of 3363) were collected from 12 laboratories. Sera were also collected for measurements of iron, UIBC, TIBC, and ferritin for use in the latent abnormal values exclusion (LAVE) method. The blood samples were analysed within 2 hours in each laboratory using Cell Dyn and Ruby (Abbott), LH780 (Beckman Coulter), or XT-2000i (Sysmex). A panel of freshly prepared blood from 40 healthy volunteers was measured in common to assess any analyser-dependent bias in the measurements. The SD ratio (SDR) based on ANOVA was used to judge the need for partitioning RVs. RIs were computed by the parametric method with/without applying the LAVE method.ResultsAnalyser-dependent bias was found for basophils (Bas), MCHC, RDW and MPV from the panel test results and thus those RIs were derived for each manufacturer. RIs were determined from all volunteers’ results for WBC, neutrophils, lymphocytes, monocytes, eosinophils, MCV, MCH and platelets. Gender-specific RIs were required for RBC, haemoglobin, haematocrit, iron, UIBC and ferritin. Region-specific RIs were required for RBC, haemoglobin, haematocrit, UIBC, and TIBC.ConclusionsWith the novel use of a freshly prepared blood panel, manufacturer-specific RIs’ were derived for Bas, Bas%, MCHC, RDW and MPV. Regional differences in RIs were observed among the 7 regions of Turkey, which may be attributed to nutritional or environmental factors, including altitude.

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“…With a group size of less than 120 people and an abnormal distribution of laboratory parameters, a calculation is used, according to which 90% of healthy people show "normal" laboratory parameters and 10% of healthy people -"abnormal". With a group size of more than 120 people and a normal distribution of laboratory parameters, use the calculation of reference intervals in the form of Xsr ± 1.96SD, according to which 95% of healthy people are found to have "normal" laboratory parameters and 5% of healthy people are "abnormal" [7,8,9]. Reference limits are determined by factors of interindividual biological variation with respect to analytical (1) The established reference intervals for blood parameters are assumed to be multivariate, obtained by the combined processing of several laboratory parameters in the same group under the assumption of a normal distribution of indicators.…”

Section: Reference Intervals and Their Establishmentmentioning

confidence: 99%

Establishment of reference intervals blood parameters wintering operators based measurement uncertainty

Vyacheslav

Olena

Bezvershniuk

2019

2019 IEEE 8th International Conference on Advanced Optoelectronics and Lasers (CAOL)

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The research of the psychophysiological state of winterers of the Antarctic station "Academician Vernadsky" was carried out in order to establish the feasibility of their subsequent stay at the station and fulfill their service tasks. Short-term stay of polar explorers under difficult conditions causes stressful effects and nonspecific adaptive reactions. Their organisms must adapt to the exposure of various extreme factors and, in the first turn, natural ones. Considering that hematopoiesis is an important mechanism on which the ultimate adaptive result depends, a study of blood parameters was performed. It has been found that there are some changes in certain blood parameters that are not relevant with the reference norms for the studied polar groups. In this connection, there is a problem in adjusting the reference intervals for them. In order to determine the reference intervals, it is necessary to take into account the uncertainty of their establishment, having previously analyzed the sources of uncertainty.

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A survey of Australian haematology reference intervals

Cited by 10 publications

References 3 publications

Indirect reference intervals for haematological parameters in capillary blood of pre-school children

Indirect reference intervals for haematological parameters in capillary blood of pre-school children

A nationwide multicentre study in Turkey for establishing reference intervals of haematological parameters with novel use of a panel of whole blood

Establishment of reference intervals blood parameters wintering operators based measurement uncertainty

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