A Survey on the Frequency of HLA-B27 in Patients Engaged With Seronegative Spondyloarthropathies in Kashan, Iran

Esalatmanesh, Kamal; Taghadosi, Mohsen; Arj, Abas

doi:10.17795/zjrms983

Cited by 3 publications

(6 citation statements)

References 9 publications

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“…21 HLA-B27 was found to be more commonly associated with AS (62.8%) followed by ReA (21.2%) and PsA (12.2%) in our study. Kamal et al, 2 observed that HLA-B27 was predominantly associated with AS (69%) similar to our study.…”

Section: Discussionsupporting

confidence: 92%

“…Spondyloarthritis (SpA) is a group of inflammatory arthritis, with common clinical manifestations, etiological factors and genetic characteristics, with a strong link to class I HLA antigens. [1][2][3] This group of diseases is characterized by its axial involvements and absence of rheumatoid factor, 4 frequently affecting sacroiliac (SI) joints of the spine that connects spine with the pelvis. SpA also has predispose to major inflammation in joints throughout the body.…”

Section: Introductionmentioning

confidence: 99%

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Association of Genetic Marker HLA-B27 with Spondyloarthritis In A Tertiary Care Centre In South India

Thulukanam¹,

Muthamilan²,

Leela³

et al. 2022

J. Pure Appl. Microbiol.

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Human leukocyte antigens (HLA) are gene products found in the major histocompatibility complex, aiding in differentiation of “self” from “non-self” antigens, and is crucial for the communication between immune cells of the human body. HLA-B27, in specific, has a strong interrelation with different types of Spondyloarthritis (SpA). The aim of this study is to study the frequency of HLA-B27 in suspected Spondyloarthritis patients presenting with axial and peripheral joint involvement, who attended our tertiary care centre from August-2017 to January-2021. Patients fulfilling Assessment of Spondyloarthritis International Society (ASAS) criteria for Spondyloarthritis were included in the study, and were further classified into Spondyloarthritis sub-groups. Blood samples were collected for CRP and ESR tests along with HLA-B27 detection by RT-PCR method. Magnetic resonance imaging was done for sacroiliac joints in patients with lower back pain. Analysis of 289 samples of SpA patients revealed 156 (54%) to be HLA-B27 positive and 133 (46%) to be HLA-B27 negative. There were 98 patients (63%) with Ankylosing spondylitis, 33 (21%) had Reactive arthritis, 19 (12%) had Psoriatic arthritis, 6 (4%) had Undifferentiated Spondyloarthritis, and inflammatory bowel disease was diagnosed in 0% in HLA-B27 positive Spondyloarthritis patients. The frequency of HLA-B27 among the Spondyloarthritis (SpA) patients in our study was found to be 54% (156), more common clinical manifestation in men belonging to the age group of 16-25 years positive patients. Ankylosing spondylitis (AS) was found to be the most common sub-groups observed among the SpA patients.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Association of Genetic Marker HLA-B27 with Spondyloarthritis In A Tertiary Care Centre In South India

Thulukanam¹,

Muthamilan²,

Leela³

et al. 2022

J. Pure Appl. Microbiol.

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“…HLA-B27 has a long history of being strongly genetically linked to AS. 34,35 In our investigation, HLA-B27 was shown as frequently linked to AS (62.8%) than to ReA (21.2%) or PsA (12.2%), similar results stated by Kamal et al 2 that HLA B27 mainly was related to AS (69%). After rheumatoid arthritis, the second most prevalent inflammatory arthritis in India is spondyloarthritis.…”

Section: Discussionsupporting

confidence: 84%

“…Spondyloarthritis (SpA) is a subset of inflammatory arthritis with a significant genetic correlation to class I HLA antigens with clinical manifestations and etiological variations. [1][2][3] This group of illnesses usually affects the sacroiliac (SI) joints, and they are distinguished by their axial involvement and lack of rheumatoid factor. 4 SpA also prefers to affect the body's key joints, and along the length of the spine, it produces pain, stiffness, and twinging (suffer a sudden, sharp localized pain).…”

Section: Introductionmentioning

confidence: 99%

Correlation of Interleukin-17 and 23 Inflammatory Markers with Genetically Transmitted Spondyloarthritis Patients at a Tertiary Care Facility, South India

Thulukanam¹,

Leela²,

Venkatesan³

et al. 2023

J. Pure Appl. Microbiol.

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Human leukocyte antigens (HLA) are genetically derived proteins in the major histocompatibility complex. They help distinguish “self” and “non-self” antigens and are essential in interacting with the immune cells inside the body. The present research work examines the prevalence of HLA-B27 among patients suspected of Spondyloarthritis (SpA), which has also been correlated with Interleukin-17/23 Inflammatory Markers and other clinical manifestations and was carried out between August 2017 to January 2021. The patient’s blood samples were collected and tested for HLA-B27 and Interleukin-17/23 inflammatory markers. Among 289 SpA patients, 60% (172) were males, and 40% (117) were females, with a ratio of 1.5:1. Ankylosing Spondylitis (65.1%) was found to be the most prevalent subgroup of SpA among the patients, closely followed by reactive arthritis (21%), psoriatic arthritis (10.7%), undifferentiated spondyloarthritis (2.1%), and inflammatory bowel disease with associated arthritis (1%). HLA-B27 was found to be positive in 54% (156) out of 289 patients. Normal IL-17 ranges were seen in 42% of HLA-B27- positive patients, while increased IL-17 was seen in 58% of the population with positive HLA-B27 cases. IL-23 was found within normal ranges in 40% of positive HLA-B27 cases, while it was found to be increased in 60% of the positive HLA-B27 positive subjects. We concluded that HLA-B27 was found to be positive among more than half of the patient population with SpA. The early detection of HLA-B27 may aid in changing lifestyle to prevent Spondyloarthritides.

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“…Meanwhile, B*2746, B*2749, B*2767, B*2702 and B*2705 subtypes were detected among Ankylosing Spondylitis patients in Turkey [32]. HLA-B27 was shown to have highest prevalence in ankylosing spondylitis and least prevalence in juvenile rheumatoid arthritis among Iranian population [33]. Studies reported an increased prevalence of HLA-B27 in rheumatoid arthritis patients in Turkey [34].…”

Section: Discussionmentioning

confidence: 95%

Association of HLA-B27 Gene and Rheumatoid Arthritis: Analysis of Potential Role as a Predictive Biomarker

Khidhir,

Ahmad,

Sabir

2023

KJAR

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Rheumatoid arthritis (RA) is a predominant inflammatory arthritis in human. The function of HLA-B27 gene in other types of arthritis has been studied, however, its function in RA is unclear. This study investigated the relative expression of HLA-B27 gene in RA patients compared to normal control and assesses its suitability as a biomarker for early detection of RA. Bioinformatics analysis was used to determine the profile of HLA-B27 gene in different human tumors to demonstrate the possible involvement of HLA-B27 in both RA and cancer; and also, to discover its functional association with other human genes. Samples of human blood from RA patients and healthy individuals were collected, and RNA extraction, cDNA synthesis and qPCR were carried out to detect relative expression of HLA-B27. ROC analysis was undertaken to investigate HLA-B27 diagnostic performance; GENT2 platform was used to compare HLA-B27 expression levels in different human tumors, and gene-gene interaction network was generated using GeneMANIA to identify correlation of HLA-B27 with other human genes. The qPCR analysis demonstrated an increase in the HLA-B27 expression by 1.65 fold in RA compared to normal control. ROC analysis indicated that HLA-B27 expression could efficiently differentiate RA from normal, supporting its potential use as diagnostic molecular biomarkers. The GENT2 revealed that HLA-B27 expression levels vary across different tumor types, most notably in heart tissue. The gene-gene interaction network revealed that KIR3DL1, KIR3DS1, LILRB1, B2M and LILRA1 were the leading genes showing the highest correlations with the HLA-B27. Our results indicate that HLA-B27 gene is involved in the RA pathogenesis and it can be used as a molecular biomarker for the diagnosis of RA. Our findings could lead to the discovery of novel diagnostic, preventive and therapeutic strategies.

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A Survey on the Frequency of HLA-B27 in Patients Engaged With Seronegative Spondyloarthropathies in Kashan, Iran

Cited by 3 publications

References 9 publications

Association of Genetic Marker HLA-B27 with Spondyloarthritis In A Tertiary Care Centre In South India

Association of Genetic Marker HLA-B27 with Spondyloarthritis In A Tertiary Care Centre In South India

Correlation of Interleukin-17 and 23 Inflammatory Markers with Genetically Transmitted Spondyloarthritis Patients at a Tertiary Care Facility, South India

Association of HLA-B27 Gene and Rheumatoid Arthritis: Analysis of Potential Role as a Predictive Biomarker

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