A topical ointment formulation containing leaves' powder of Lawsonia inermis accelerate excision wound healing in Wistar rats

Abstract

<jats:p>Aim: Lawsonia inermis (LI), a naturally grown or cultivated shrub in Northeast of Africa and India, has been traditionally used as a strong remedy for several injuries. However, few studies have reported its use as a cicatrizing agent. The aim of this study was to evaluate the effect of daily application of an ointment prepared with LI leaves' powder on wound healing in Wistar rats. Materials and Methods: Twenty female Wistar rats were used in this study. Excisional wound model was realized by removing skin from the dorsal part of the neck of each animal. Wounds have been then treated by a daily application of LI ointment prepared by mixing leaves' powder to petroleum jelly in test group and by simple application of petroleum jelly in control group. Evaluation of wound healing activity was then based on calculating the percentage of wound contraction, period of epithelialization, and wound index every 3 days for a period of 24 days, then, a histological study of the healed excised wound was performed. Results: Treatment with LI has shown excellent wound healing activity, since it has increased percent of wound contraction, and reduced period of epithelialization and wound index as compared to control (p&lt;0.05). These results have been supported by the histological findings that revealed better epithelialization, dermal differentiation, collagen fiber orientation, and angiogenesis in LI treated rats compared to control (p&lt;0.05). Conclusion: We can conclude that LI leaves' can be used as a potential wound healing agent.</jats:p>

Wound healing by topical application of Momordica charantia L. formulations on mice

“…The use of traditional medicinal plants for wound healing is based on their antiseptic, astringent, anti-inflammatory, and antimicrobial properties [ 37 ]. This antimicrobial activity is related to facilitating the wound healing process, since open wounds are particularly prone to infections, especially by bacteria, and they also provide an entry point for systemic infections [ 25 ].…”

Application of Fish Collagen-Nanochitosan-Henna Extract Composites for the Control of Skin Pathogens and Accelerating Wound Healing

“…The incorporation or individual utilization of He had significant positive impacts in wound's contraction and epithelialization in rats. He has treasures from bioactive compounds, which could contribute to healing processes of wounded skins; the combined synergistic actions of multiple He constituents are assumed to correlate with its curative beneficial effects [35]. The He phytoconstituents include phenolic amalgams (e.g., coumarins, naphthalenes, lignans, alkylphenones, xanthones, naphthoquinones, tannins, and flavonoids), alkaloids, terpenes, and steroids [28,29,34].…”

“…Additionally, the He flavonoids could augment skin WH via multiple actions, e.g., their antibacterial, astringent, cell necrosis prevention, cytokine expression modulation during inflammation, angiogenesis improvement, and prostaglandin synthesis inhibition [25,29,33,70]. The He tannins' contents were also documented for contributing to WH through enhancing tissue organization and regeneration; the He tannin actions mainly depend on their astringent, antioxidant, antibacterial, anti-inflammatory, and free radical biochelation activities [28,34,35,72]; these actions frequently lead to stimulate keratinocyte/fibroblast proliferation, improve WH and angiogenesis, and develop rapid crust via proteins' precipitation in damaged tissue [29,30,35]. The He-loaded nanopolymers, e.g., PLA and gelatin nanofibers, showed additional capabilities for controlling wound infections and WH via formation of hygienic dressings [37].…”

“…As henna contains treasures of bioactive constituents (e.g., lawsone, naphthoquinone, coumarins, phenolics, flavonoids, alkaloids, tannins, quinones, terpenoids, and xanthones), these plant derivatives were experimented in numerous medicinal, pharmaceutical, and biomedical applications (including their hypoglycemic, immune stimulant, analgesic, hepatoprotective, anticancer, anti-inflammatory, antimicrobial, antidermatophytic, antiparasitic, antisickling, antitrypanosomal, antioxidant, allelopathic, and wound healing activities) [29][30][31][32]. The WH activity and reducing inflammation and infections of wounded skins were reported from henna extracts (He) [30,[33][34][35]. The fabrications of wound dressing/WH accelerator formulations based on biopolymers/nanopolymers and plants' extracts were suggested as effectual treatments for healing injured tissues and prevent their inflammation/infections, either individually or in composited forms [3,23,[36][37][38].…”

The analgesic and anti-inflammatory effect of lawsone isolated from Lawsonia inermis

“…The pharmacological studies on Lawsonia inermis revealed antibacterial activity [6,7], antifungal [8,9], antiparasitic [10,11], molluscicidal [12], antioxidant [13,14], hepatoprotective [15,16], central nervous [17], analgesic [18,19], anti-inflammatory [20,21], antipyretic [18], wound [22,23] and burn healing [24], immuno-modulatory [25,26], anti-urolithiatic [27,28], antidiabetic and hypolipidemic [29,30], antiulcer [31], antidiarrhoeal [19] , diuretic [32], anticancer [33,34] and many other pharmacological effects.…”